[Prevention of postoperative adhesions--animal experiment study]. / Zur Prophylaxe post-operativer Adhäsionen--Eine tierexperimentelle Studie.

Standardised lesions were placed on both uterine horns and on the abdominal wall of seventy-five female rabbits. The animals were divided into five groups consisting of 15 animals each. Before closure of the peritoneum the following drugs were applied in the peritoneal cavity: Group I received NaCl (control), Group II received 10 ml poly-(1-vinyl-1-pyrrolidone)-iodine complex (Betaisodona), Group III received 100 000 I. U. streptokinase and 25 000 I. U. streptodornase (Varidase), Group IV received 100 000 KIE approtinine (AntagosanA) and Group V received triamcinolone acetonide (Volon A-Haftsalbe). Two weeks after surgery the animals were relaparotomised and the situs was inspected. Quality and extent of the adhesions were assessed and documented. The results obtained in group II did not differ from controls, while the remaining groups showed significantly fewer adhesions. The best results were obtained in animals receiving Volon A-Haftsalbe (Group V) where 86% fewer adhesions were observed.

[Investigations into methionine metabolism in normal and disturbed gestation on the basis of animal experiments]. / Untersuchungen zum Methioninmetabolismus bei normaler und gestörter Gestation--eine tierexperimentelle Studie.

One hundred and fifty Han Wistar rats, divided into three groups, non-pregnant controls, pregnant animals and pregnant animals with disturbed gestation, were each given an injection of 10 muCi 75-Se-methionine, administered into a tail vein--in the case of the two pregnant groups on the 20th day of gestation. The disturbance of gestation in the third group had been caused by ligating the uterine vessels on the 17th day of gestation. This resulted in a reduction in the number of live fetuses and a significant growth retardation in surviving fetuses which had lain close to the site of the ligature. Ten animals of each group were killed 30, 60, 180, 360 and 1440 minutes after the methionine injection and the activity in the blood, liver and fetoplacental unit was measured. From the measured activity (divided by the specimen weight and multiplied by the total weight of the animal divided by the activity of the injected dose) the relative organ activity was calculated; its duration may be regarded as a measurement of the nature and extent of methionine metabolism. It was found that the increase in amino-acid metabolism (especially in the liver) which takes place in pregnancy is less pronounced in cases of disturbed gestation. In the fetoplacental unit a high level of transmethylation activity was found. When gestation is disturbed, transmethylation is also increased, while in contrast a reduced rate of protein synthesis was observed. The division of the unit into fetus and placenta shows that the high transmethylation activity only takes place in the fetus, corresponding to the enzyme pattern in the fetus and placenta. The redistribution of fetal methionine utilization in favor of transmethylation where gestation is disturbed is systemically controlled. Simultaneously there is a localized, circulation-dependent, absolute reduction in diaplacental transport.

[Adhesion prophylaxis - a comparative experimental animal study]. / Adhäsionsprophylaxe - Eine vergleichende tierexperimentelle Studie.

A number of 45 female rabbits were anesthetized, and intra-abdominal lesions were set at either of the uterine horns and the abdominal wall in a standardized technique. Before closure of the abdomen the collective--divided into three subgroups of 15 animals each--received i.p. one of the following pharmaca: Group I: solution of physiologic saline (control group) Group II: poly-1-vinyl-2-pyrollidon iodine complex Group III: streptokinase/streptodornase. Two weeks after the first operation a second laparotomy was performed, and the sites were inspected. The adhesions were examined and their quantity and quality documented. Statistical evaluation showed that groups I and II were significantly different from group III as to the extent and intensity of the adhesions.

[Computertomograph detection of a postoperative iliac retroperitoneal hematoma (author's transl)]. / Diagnose eines postoperativen Hämatoms im Bereich des Musculus iliacus mittels Computertomographie.

Following the operation of the fixation of the vaginal vault by the Williams-Richardson technique a retroperitoneal hematoma was diagnosed and localized in the region of right iliac muscle using computer tomography. The patient has been under heparin therapy because of suspected pulmonary embolism.

[On the clinical significance of sequential scintigraphic examinations of the uteroplacental perfusion (author's transl)]. / Zur klinischen Bedeutung sequenzszintigraphischer Untersuchungen der utero-plazentaren Perfusion.

The utero-placental perfusion was examined by means of the method of Hünermann (1972) in a group of 74 patients with toxemia of pregnancy, diabetes mellitus, or intrauterine growth retardation. The perfusion measurements were categorized and evaluated according to the clinical findings. The correlation between single values deriving from the time-activity curves and their clinical predictive value was ascertained. A strong correlation between the parameters and the clinical findings resulted when several criteria were considered simultaneously. Among the single values resulting from the perfusion curves, the Tmax (placenta) and Tmax (uterus) (time of activity maximum) as well as the quotient of both revealed the highest significance. Pathological time-activity-curves were found in 16 of 19 patients with moderate and severe intrauterine growth retardation, and in all patients with moderate and severe toxemia, or diabetes mellitus. The limited clinical value of the used method is discussed.

[Studies on amino acid kinetics in rats without and with interference of the pregnancy using 75-Se-methionine (author's transl)]. / Tierexperimentelle Untersuchungen zur Aminosäurekinetik bei ungestörter und gestörter Schwangerschaft mit 75-Se-Methionin.

A collective of 60 rats was divided into 3 groups of 20 non-pregnant, 20 pregnant without interference of the pregnancies and 20 pregant with ligature of the uterine vessels to induce an intrauterine growth retardation. The organ distribution of 100 muC intravenously injected 75-Se-methionine, which is actively transported from the placenta into the fetal circulation, was computer-scintigraphically measured. After 30 minutes and 6 hours typical differences, amongst the 3 groups, between the liver and foetoplacental unit methionine uptake were demonstrated both visually and quantitatively. On the basis of this experimental animal model, it should be attempted to measure quantitatively the placental disorders which are not caused primarily by perfusion of the uteroplacental unit.

[Prevention of idiopathic respiratory distress syndrome. Influence of betamethasone on the frequency of respiratory distress syndrome in a premature infant collective at the Bonn University Gynecologic Clinic]. / Zur Prophylaxe des idiopathischen Atemnot-Syndroms. Der Einfluss von Betamethason auf die Frequenz des idiopathischen Atemnot-Syndroms (RDS) im Frühgeborenen-Kollektiv der Universitäts-Frauenklinik Bonn

Out of a group of 189 pregnant women, who were in the 28th to 37th week of gestation, 57 women received i.m. injections of betamethasone. 132 premature infants whose mothers were not treated served as controls. Betamethasone caused a significant decrease in established respiratory distress syndrome (RDS). In the betamethasone group there was an incidence of 7% of respiratory distress syndrome as compared to 23,5% in the control group. Neonatal mortality was 1,8% in the treated group versus 10,6% in the control group. The occurrence of suspected respiratory distress syndrome also was significantly lowered after betamethasone. In the control group premature rupture of membranes or administration of tocolytic drugs did not have any effect on the frequency of respiratory distress syndrome. However, the premature babies who were delivered by caesarian section had a significantly higher incidence of respiratory distress syndrome than those who were delivered vaginally. After betamethasone treatment the occurrence of respiratory distress syndrome after caesarian section was as low as in the group with vaginal delivery. The present study of a larger group of premature babies has confirmed previous positive results of betamethasone treatment.