Algal macromolecular mediated synthesis of nanoparticles for their application against citrus canker for food security.

Citrus canker is a disease of economic importance and there are limited biocontrol agents available to mitigate it in an integrated manner. This study was conducted to combat citrus canker disease using biologically active nanoparticles (Ag, Cu and ZnO and 300, 900, 1200, and 1500 ppm) synthesized from macromolecules extracted from alga, Oedogonium sp. The synthesis of the nanoparticles was confirmed by UV-Vis Spectroscopy, FTIR, SEM, XRD, and DLS Zeta sizer while their efficacy was tested against Xanthomonas citri by measuring zone of inhibition. Results indicated that Ag and Cu nanoparticles at 1200 ppm exhibit the highest activity against Xanthomonas citri, followed by ZnO at 1500 ppm. The minimum inhibitory concentrations (MIC) of Ag, Cu and ZnO NPs were 1, 2 and 10 mg mL-1, respectively while minimum bactericidal concentrations (MBC) were for Ag and Cu 2, 4 mg mL-1 and for ZnO NPs more then 10 mg mL-1, were required to kill the X. citri. Bacterial growth respectively. Macromolecules extracted from algal sources can produce nanoparticles with bactericidal potential, in the order of Ag > Cu > ZnO to mitigate citrus canker disease and ensuring sustainable food production amid the growing human population.

The potential of zeolite nanocomposites in removing microplastics, ammonia, and trace metals from wastewater and their role in phytoremediation.

Nanocomposites are emerging as a new generation of materials that can be used to combat water pollution. Zeolite-based nanocomposites consisting of combinations of metals, metal oxides, carbon materials, and polymers are particularly effective for separating and adsorbing multiple contaminants from water. This review presents the potential of zeolite-based nanocomposites for eliminating a range of toxic organic and inorganic substances, dyes, heavy metals, microplastics, and ammonia from water. The review emphasizes that nanocomposites offer enhanced mechanical, catalytic, adsorptive, and porosity properties necessary for sustainable water purification techniques compared to individual composite materials. The adsorption potential of several zeolite-metal/metal oxide/polymer-based composites for heavy metals, anionic/cationic dyes, microplastics, ammonia, and other organic contaminants ranges between approximately 81 and over 99%. However, zeolite substrates or zeolite-amended soil have limited benefits for hyperaccumulators, which have been utilized for phytoremediation. Further research is needed to evaluate the potential of zeolite-based composites for phytoremediation. Additionally, the development of nanocomposites with enhanced adsorption capacity would be necessary for more effective removal of pollutants.

Enhancing tomato plant growth in a saline environment through the eco-friendly synthesis and optimization of nanoparticles derived from halophytic sources.

Using green synthesis methods to produce halophytic nanoparticles presents a promising and cost-effective approach for enhancing plant growth in saline environments, offering agricultural resilience as an alternative to traditional chemical methods. This study focuses on synthesizing zinc oxide (ZnO) nanoparticles derived from the halophyte Withania somnifera, showcasing their potential in ameliorating tomato growth under salinity stress. The biosynthesis of ZnO nanoparticles was initially optimized (i.e., salt concentration, the amount of plant extract, pH, and temperature) using a central composite design (CCD) of response surface methodology (RSM) together with UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), and dynamic light scattering (DLS) to comprehensively characterize the biosynthesized ZnO NPs. The central composite design (CCD) based response surface methodology (RSM) was used to optimize the biosynthesis of ZnO nanoparticles (NPs) by adjusting salt concentration, plant extract, pH, and temperature. The ZnO NPs were characterized using UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), and dynamic light scattering (DLS). FT-IR showed an absorption peak of ZnO between 400 and 600 cm-1, while SEM showed irregular shapes ranging between 1.3 and 6 nm. The data of EDX showed the presence of Zn (77.52%) and O (22.48%) levels, which exhibited the high purity synthesized ZnO under saline conditions. Introducing ZnO nanoparticles to tomato plants resulted in a remarkable 2.3-fold increase in shoot length in T23 (100 mg/L ZnO nanoparticles + 50 mM NaCl). There was an observable increase in foliage at T2 (20 mg L-1 ZnO) and T23 (100 mg L-1 ZnO-NPs + 50 mM NaCl). Tomato plants treated with T2 (20 mg L-1 ZnO) and T23 (100 mg L-1 ZnO-NPs + 50 mM NaCl) improved root elongation compared to the control plant group. Both fresh and dry leaf masses were significantly improved in T1 (10 mg L-1 ZnO) by 7.1-fold and T12 (10 mg L-1 ZnO-NPs + 100 mM NaCl) by 0.8-fold. The concentration of Zn was higher in T12 (10 mg L-1 ZnO NPs + 100 mM NaCl) among all treatments. Our findings prove that utilizing ZnO nanoparticles under saline conditions effectively promotes tomato plants' growth, thereby mitigating the negative impacts of salt stress.

Efficient detoxification of textile wastewater by applying Chenopodium album nanoparticles and its application in simulated metal-bearing effluents removal.

Large-scale pollution of water and soils bodies is associated with the discharge of the untreated textile industry effluents. Halophytes grows on saline lands and accumulate secondary metabolites and other stress protective compounds. Utilization of Chenopodium album (halophytes) to synthesize zinc oxide (ZnO) and their efficiency to treat different concentrations of textile industry waste water is proposed in this study. Potential of nanoparticles textile industry waste water effluents was also analyzed by exposing different concentrations of nanoparticles (0 (control), 0.2, 0.5, 1 mg) and time intervals of 5, 10, and 15 days. The absorption peaks by UV region, FTIR and SEM analysis were used characterized on ZnO NPs for the first time. FTIR analysis showed the preens of various functional groups and vital phytochemicals that can play its role in the formation of nanoparticles that can be used for trace elements removal and bioremediation. SEM analysis indicated that the pure ZnO NPs synthesis ranged from 30 to 57 nm. Results shows that the green synthesis of halophytic nanoparticles presents maximum removal capacity after 15 days exposure to 1 mg of ZnO NPs. Hence, the prepared ZnO Nps from halophytes can be a viable solution for treating the textile industry effluents before they are discharged into water bodies for sustainable environmental growth and environmental safety.

Biochar-plant interaction and detoxification strategies under abiotic stresses for achieving agricultural resilience: A critical review.

The unpredictable climatic perturbations, the expanding industrial and mining sectors, excessive agrochemicals, greater reliance on wastewater usage in cultivation, and landfill leachates, are collectively causing land degradation and affecting cultivation, thereby reducing food production globally. Biochar can generally mitigate the unfavourable effects brought about by climatic perturbations (drought, waterlogging) and degraded soils to sustain crop production. It can also reduce the bioavailability and phytotoxicity of pollutants in contaminated soils via the immobilization of inorganic and/or organic contaminants, commonly through surface complexation, electrostatic attraction, ion exchange, adsorption, and co-precipitation. When biochar is applied to soil, it typically neutralizes soil acidity, enhances cation exchange capacity, water holding capacity, soil aeration, and microbial activity. Thus, biochar has been was widely used as an amendment to ameliorate crop abiotic/biotic stress. This review discusses the effects of biochar addition under certain unfavourable conditions (salinity, drought, flooding and heavy metal stress) to improve plant resilience undergoing these perturbations. Biochar applied with other stimulants like compost, humic acid, phytohormones, microbes and nanoparticles could be synergistic in some situation to enhance plant resilience and survivorship in especially saline, waterlogged and arid conditions. Overall, biochar can provide an effective and low-cost solution, especially in nutrient-poor and highly degraded soils to sustain plant cultivation.

Antimicrobial activity of comfort related properties of silk treated with herbal extracts in making of reusable masks

The study was aimed at divulging an eco-friendly antimicrobial finish on 100 % silk woven fabric. The leaves' extract of Azadirachata indica, Butea monosperma and Litche chinensis were used as the development of eco-friendly antimicrobial finish. The antimicrobial property and comfort related property were checked before and after applying antimicrobial finish. In comfort related property absorbency & air permeability were checked. The ASTEM E2149 Shake Flask method was used to check antimicrobial finish and AATCC method was used for checking fabric property. One way ANOVA statistical test was applied for analysis of results. The FTIR and SEM results showed the presences of finish on fabrics. In comfort related property, absorbency and air permeability was increased. The results showed that antimicrobial finish made 100% reduction against microorganism up to 25 washes which can be used in making reusable masks fight against COVID- 19.

Impact of Nanomaterials on the Regulation of Gene Expression and Metabolomics of Plants under Salt Stress.

Plant salinity resistance results from a combination of responses at the physiological, molecular, cellular, and metabolic levels. This article focuses on plant stress tolerance mechanisms for controlling ion homeostasis, stress signaling, hormone metabolism, anti-oxidative enzymes, and osmotic balance after nanoparticle applications. Nanoparticles are used as an emerging tool to stimulate specific biochemical reactions related to plant ecophysiological output because of their small size, increased surface area and absorption rate, efficient catalysis of reactions, and adequate reactive sites. Regulated ecophysiological control in saline environments could play a crucial role in plant growth promotion and survival of plants under suboptimal conditions. Plant biologists are seeking to develop a broad profile of genes and proteins that contribute to plant salt resistance. These plant metabolic profiles can be developed due to advancements in genomic, proteomic, metabolomic, and transcriptomic techniques. In order to quantify plant stress responses, transmembrane ion transport, sensors and receptors in signaling transduction, and metabolites involved in the energy supply require thorough study. In addition, more research is needed on the plant salinity stress response based on molecular interactions in response to nanoparticle treatment. The application of nanoparticles as an aspect of genetic engineering for the generation of salt-tolerant plants is a promising area of research. This review article addresses the use of nanoparticles in plant breeding and genetic engineering techniques to develop salt-tolerant crops.

Cloning and over Expression Studies of Ovine Somatotropin cDNA of Kajli (sheep breed) in a Prokaryotic System.

Genetic studies including the quest, cloning and expression of genes encoding proteins responsible for various vital physiological processes and beneficial characteristics of economic perspective have made the biotechnology research progressively auspicious. Due to its great zootechnical and industrial importance somatotropin gene have been cloned from various animal species. Current study was designed to clone mature ovine growth hormone complementary DNA (oGH cDNA) of a sheep breed, Kajli and carry out over expression studies of cloned GH cDNA in a suitable prokaryotic expression system. Sheep GH cDNA was cloned in T/A (thymine / adenine) vector with signal peptide and confirmed by nested polymerase chain reaction (PCR) and restriction digestion. The gene was then ligated in pLEX expression vector and restricted plasmids showed a fragment insert of ~ 600 bps. Restriction analysis confirmed positive clones, were induced for protein expression analysis. The pET vectors (plasmid for expression by T7 RNA polymerase) have an isopropylthio-ß-galactoside (IPTG) inducible strong T7 promoter and Escherichia coli expression strain of BL21 (DE3) pLysS contains DNA fragment from T7 phage which harbors RNA polymerase. Therefore, for expressing recombinant proteins, cells were induced with various IPTG concentrations to optimize expression levels. Cells were induced with different IPTG concentrations (0.1 to 0.8 mM) followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Results indicated maximum expression level of oGH at 5 hrs after induction of cells with 0.3 mM IPTG concentration with a molecular weight of 22 kDa. As for as cellular localization of protein is concerned accumulation of expressed oGH is observed in inclusion bodies. The successful expression of the cloned GH cDNA of sheep confirmed the functional viability of the clone. The above mentioned technique of genetic engineering has provided to boost the dairy industry by the production of large quantities of recombinant bovine somatotropin (rbST).

The role of halophytic nanoparticles towards the remediation of degraded and saline agricultural lands.

Salinity is one of the major causes of abiotic stress that leads to a reduction in crop yield. One strategy to alleviate and improve crop yield is to use halophytes. These types of plants naturally produce bioactive secondary metabolites, proteins, carbohydrates, and biopolymers that are involved in specialized physiological adaptation mechanisms to alleviate soil salinity. These traits could be leveraged and, in turn, be the focus of future breeding programs aimed to improve salinity resistance in traditional crops. Recently, the field of nanotechnology has gained the attention of researchers involved in agricultural science and associated disciplines. However, information on salinity tolerance mechanisms of halophytes, based on nanoparticles in agricultural crop plants, is limited. Recently, the use of selected halophytic-based nanoparticles has shown to improve crop performance by enhancing the plants' ion flux, improving water efficiency, root hydraulic movement in the favor of plant photosynthesis, the production of proteins involved in oxidation-reduction reactions, reactive oxygen species (ROS) detoxification, and hormonal signaling pathways under stress. Therefore, the aim of this review is to highlight the application of halophytic nanoparticles in alleviating salt stress in plants by understanding the mechanisms of plant growth, water relation, ion flux, photosynthesis, and the antioxidant defense system. This review also addresses uncertainties, ecotoxicological concerns, and associated drawbacks of nanoparticles on the environment. Future research perspectives with respect to the sustainable usage of nanoparticles in saline agriculture have also been presented.

Molecular characterisation of Bacillus chitinase for bioconversion of chitin waste.

In this work chitin was extracted chemically from shrimp shells. Seventeen Bacillus isolates were screened for chitinolytic activity. The chitinolytic strains of Bt. were screened at different temperatures and pHs for their hydrolytic potentials. By using a pair of specific primers, endochitinase gene was amplified from SBS Bt-5 strain through PCR, and then cloned into pTZ57 TA cloning vector and transferred in Escherichia coli DH5α strain. The sequenced gene (GenBank Accession No: HE995800) consists of 2031 nucleotides capable of encoding 676 residues. The protein consisted of three functional domains with a calculated molecular mass of 74.53 kDa and a pI value of 5.83. The amino acid sequence of chi gene showed 99% similarity to the genes of Bt MR11 endochitinase, Bt serovar kurstaki chitinase (kchi), Bt strain MR21 endochitinase and Bacillus cereus B4264.

Mass cultivation of various algal species and their evaluation as a potential candidate for lipid production.

Microalgae have been proposed as a promising source for biodiesel production. Focusing on algal strains for biodiesel production, efforts should be made to search new strains. Experiments were carried out to investigate the effects of growth parameters (nutrients, pH, light, aeration and temperature) and the oil percentage of eight algal strains (Chlorella sp., Cladophora sp., Hydrodictylium sp., Oedogonium sp., Oscillatoria sp., Spirogyra sp., Stigeocolonium sp., Ulothrix sp.). Results show that 6.5-7.5 is the optimum pH for the growth of all algal species. Temperature showed a greater variation (25°40°C). Ulothrix sp. gave more biomass productivity and is the most suitable strain for biodiesel production due to higher oil percentage (62%). Least biomass production was observed for Stigeocolonium sp. and least oil content was obtained from Hydrodictylium sp. It was observed that among these eight algal strains for biodiesel production, Ulothrix and Chlorella are the most promising algae species.

Evaluation of antioxidant and antimicrobial potential of two endangered plant species Atropa belladonna and Matricaria chamomilla.

BACKGROUND: Plants are the natural source of antioxidants as well as antimicrobial compounds that has great potentials in pharmaceutical industry. In the present study, two medicinal plants Atropa belladonna and Matricaria chamomilla were collected from Northern areas of Pakistan. MATERIALS AND METHODS: The extracts of the collected plants were obtained by microwave assisted extraction (MAE) with changing parameters, power level and time; methanol and ethanol were solvents used during extraction. The extracts of plants were tested against different bacterial strains. RESULTS: It was observed that ethanolic extracts of Atropa belladonna has more significant antimicrobial activity against S.aureus than E.coli. In parallel, methanolic extract of Matricaria chamomilla showed greater significant antibacterial activity against S.aureus when compared with E.coli. In comparison, ethanolic extracts of Matricaria chamomilla has shown more significant results against S. aureus than E.coli (p ≤ 0.05). Both plants had no antibacterial activity against S.typhi. The free radical scavenging activity observed by DPPH assay, indicate that both plants have antioxidant activity at all levels of concentrations in solvent tested during the present work. However, methanolic extracts had greater antioxidant activity when compared with ethanolic extracts. CONCLUSION: Present study is thus helpful in highlighting present potentials for antioxidant and antimicrobial properties in the selected plants.