[Experience with high-dose immunosuppressive therapy followed by transplantation of autologous stem hematopoietic cells in patients with multiple sclerosis]. / Opyt primeneniia vysokodoznoi immunosuppressivnoi terapii s posleduiushchei transplantatsiei autologichnykh stvolovykh krovetvornykh kletok u bol'nykh rasseiannym sklerozom.

AIM: To assess efficiency of immunosuppressive therapy and subsequent autologous transplantation of stem blood cells (SBC) in patients with multiple sclerosis. MATERIAL AND METHODS: The trial enrolled 23 patients (4 men and 19 women) with multiple sclerosis (MS) lasting for 3 to 12 years. The age of the patients ranged from 18 to 44 years. The index of the progression was above 1 in all the patients. A remitting, primary-progredient, secondary-progredient course was diagnosed in 3, 3 and 17 patients, respectively. Posttransplantation follow-up was 1 to 1.5 years. The degree of the neurological deficiency (0-6 scores) was estimated by the scale of functional systems damage. Lymphocyte subpopulations were evaluated by enzyme immunoassay according to expression of membrane antigens CD3, CD4, CD8, CD16, CD20, CD25, CD56, CD95 using monoclonal antibodies ICO (Biomedspectr), humoral immunity--by serum levels of IgA, IgM and IgG. SBC mobilization was conducted for 5 days by subcutaneous introduction of neipogen (Roche) in a dose 8.7-10 mcg/kg. Preparation of SBC was made on Haemonetics blood separator on mobilization day 4-5. Cryopreservation was carried out in programmed freezer (Cryomed) with 7% dimethylsulphoxide as a cryoprotector. Pretransplantation conditioning was conducted according to the schemes BEAM + antilymphocytic globulin (protocol N 1) and fludar + melfalan + ALG (protocol N 2). RESULTS: In posttransplantation period most of the patients achieved a fall in intensity of motor and coordination disorders. No recovery of cranial nerve function was observed. The protocols of pretransplantation preparation were compared by efficiency and organic toxicity. CONCLUSION: Indications to immunosuppressive therapy in MS patients were defined, pathogenetic validation of the immunosuppressive therapy was attempted.

[Viral hepatitis A and the possible pathogenetic mechanisms of a protracted convalescence]. / Virusnyi gepatit A i vozmozhnye patogeneticheskie mekhanizmy zatianuvsheisia rekonvalestsentsii.

Metabolites of connective tissue (free serum oxyproline, protein-bound plasma oxyproline, total urine oxyproline, serum glycosaminoglycans), proteolytic enzyme elastase, proteolysis inhibitors (alpha 1-inhibitor, alpha 2-macroglobulin) were investigated in 103 patients with viral hepatitis A. It was established that connective tissue and its metabolites produce a negative effect on hepatocytic repair. Longterm convalescence is attributed to elastase hyperactivity in the presence of relative deficiency of proteolytic inhibitors.

[Dosage loads on the bone marrow in exposure to ionizing radiation]. / Dozovye nagruzki na kostnyi mozg pri vozdeistvii ioniziruiushchego izlucheniia.

The authors made calculations of the expected dose loads on the bone marrow in the population of several regions due to the disaster release of radionuclides at the Chernobyl Atomic Station as well as in professional groups subjected to ionizing radiation, in particular, those working with radium light-compounds of permanent effect. The dose loads were largest in the population of the Byelorussian ecological region. Persons working in conditions of ionizing radiation showed a functional insufficiency of hemopoiesis in correspondence with the doses but there was no synchronicity in the responses of the bone marrow and peripheral blood.

[Peptic ulcer: clinico-endoscopic and biochemical aspects of the development and healing of the peptic defect]. / Iazvennaia bolezn': kliniko-endoskopicheskie i biokhimicheskie aspekty vozniknoveniia i zazhivleniia pepticheskogo defekta.

The metabolism of collagen and glycoproteins bound with glycosaminoglycans was studied in 19 patients with a preulcerous condition (chronic primary gastroduodenitis) and in 49 patients with peptic ulcer of the stomach (12) and duodenum (37). The control groups comprised 12 practically healthy subjects (group I) and 20 patients with chronic secondary gastroduodenitis (group II). Based on the data obtained it is concluded that the high level of collagen metabolism in patients with chronic gastroduodenitis may be regarded as an early diagnostic criterion of the preulcerous condition. In patients with peptic ulcer, epithelization of the peptic defect controlled with the aid of studying the indicators of collagen formation should not be viewed as completed after clinico-endoscopic confirmation of the disease remission. There was a close correlation between the ulcerous crater area and the level of free hydroxyproline in gastric juice. High level of glycoproteins in patients with gastroduodenitis and peptic ulcer can be used as an additional criterion of the degree of the inflammatory reaction.