RESUMO
Rat liver mitochondria were stored at 0-4 degrees C for several days using an appropriate medium and energy source. The elimination of the majority of microsomes and lysosomes, that normally contaminate isolated mitochondria, had a positive effect in preservation of respiratory control, P:O ratio, and monoamine oxidase activity during long term storage.
Assuntos
Mitocôndrias Hepáticas/fisiologia , Fosfatase Ácida/metabolismo , Animais , Fracionamento Celular/métodos , Temperatura Baixa , Ácidos Graxos não Esterificados/metabolismo , Glucose-6-Fosfatase/metabolismo , Microscopia Eletrônica , Mitocôndrias Hepáticas/ultraestrutura , Monoaminoxidase/metabolismo , Fosforilação Oxidativa , Fosfolipídeos/metabolismo , Preservação Biológica , RatosRESUMO
Isolated rat liver mitochondria, freed from microsomes and lysosomes contaminants, were maintained at 0-4 degrees C for several days using an appropriate medium and energy source. It was observed that the phospholipase A2 activity of mitochondria deficient in vitamin E is higher than in normal mitochondria, and that the presence of vitamin E in the preservation medium diminishes the phospholipase A2 activity in deficient mitochondria. In vitamin E deficient mitochondria up to 45% of phospholipids was digested by the endogenous phospholipase with little loss in the energy linked function or without considerable activation of the latent enzymes monoamine oxidase and ATPase. These results are consistent with the occurrence of phospholipids in the mitochondrial membrane which would render it more accessible to the action of phospholipase A2.
