[C677T AND A1298C ALLELE POLYMORPHISM GENE OF METHYLENETETRAHYDRAFOLATEREDUCTASE IN PATIENTS WITH NONALCOHOLIC FATTY LIVER DISEASE AND TYPE 2 DIABETES].

The article presents the results of the study C677T and A1298C polymorphisms of MTHFR gene and their influence on plasma homocysteine levels in patients with nonalcoholic fatty liver disease (NAFLD). The study involved 100 patients with NAFLD and 40 apparently healthy individuals (control group). Determination of allelic polymorphism was performed by polymerase chain reaction with the detection results of hybridization by fluorescence in real-time. Determination of plasma homocysteine levels was performed by ELISA. As a result, studies have not found significant differences in the distribution of genotypes investigated C677T and A1298C MTHFR gene between patients with NAFLD and control group. We have detected statistically significant relationship between the level of homocysteine plasma C677T polymorphism of the MTHFR gene in patients with NAFLD and lack of connection with the A1298C polymorphism of the gene MTHFR.

[MORPHOFUNCTIONAL ADJUSTMENT VASCULAR AND CELLULAR COMPONENTS OF THE CEREBELLAR CORTEX IN EXPOSURE TO BODY SULFATES OF COPPER, ZINC AND IRON].

In order to analyze the morphological and morphometric reconstructions of the vascular bed, and Purkinje cells of the cerebellar cortex of rats in long-term action (for 90 days) on the body of sulphates of copper, zinc and iron, an experiment was conducted on 48 adult white male rats weighing 200-250 g in age 5-7 months. We used anatomical, morphometric, statistical and common methods of microanatomical research. It was found that the combined effect on the body of sulphates of copper and zinc, and iron in the cerebellum has enough expressive toxicity, which affects the condition of the vascular bed, and Purkinje cells. The degree of morphological transformations is in direct proportion to the duration of the experiment. In the pathogenesis of violations leading role played by hypoxia, develop signs of swelling of the cerebellar cortex with signs hemorrhagic infiltration, the severity of which is maximum on the 60th day of the experiment.

[Chemistry of galtamycin. Specification of the aglycone structure. Synthesis of the amino analog]. / Khimiia galtamitsina. Utochnenie struktury aglikona. Sintez aminoanaloga.

The structure of galtamycin aglycone was elucidated. Synthesis of its amino analog with higher antitumor activity and antibacterial activity is described.

[Study of patulin by NMR and mass spectroscopy]. / Izuchenie patulina metodami IaMR i mass-spektroskopii.

Patulin was studied by NMR and mass spectrometry. On the basis of the 1H and 13C NMR spectral analysis and experiments on double homo-(1H NMR) and heteronuclear (13C NMR) resonances complete assigning of the proton and carbon signals was achieved. Patulin was studied mass spectrometrically with using high performance mass spectrometry and the DADI technique. It was shown that formation of the [M--C2H4O]+ ion was due to rearrangement of the molecular ion (M+).

[Structure of galtamycin]. / Stroenie galtamitsina.

Structure of galtamycin, a novel anthracycline antibiotic was assessed with 1H NMR, 13C NMR and mass spectroscopy. Galtamycin includes an unusual aglycone i.e. galtamycinone containing the C-glycoside bond and glycosylated with trisaccharide consisting of two fragments of L-rodinose and one fragment of D-olivose.

[Kafamycin--a new pyrrol ether antibiotic]. / Kafamitsin--novyi pirroléfirnyi antibiotik.

Cafamycin is a novel polyether antibiotic active against gram-positive bacteria. It was isolated from the culture fluid of Streptomyces sp., an organism producing the anthracycline antibiotic galtamycin. The structure of cafamycin was assessed by spectral analysis: UV, PMR, mass spectroscopy and CD. Physico-chemical and biological properties of the antibiotic are described. Cafamycin was shown to be an analog of indanomycin (X-14547A), a pyrrol ether antibiotic.

[Mass spectrophotometric study of the antibiotic granaticin]. / Mass-spektrometricheskoe izuchenie antibiotika granatitsina.

A culture of Streptomyces sp. producing a substance with antibacterial and antitumor activity was isolated from a soil sample collected in the Gorno-Altai Autonomous Province. By its UV, NMR and mass spectra the antibiotic was identified as granaticin. Mass spectral disintegration of granaticin was studied in detail: mass spectra of electron impulse (including that of high performance), field desorption, chemical ionization of positive and negative ions. Correlation relationship between directions of fragmentation of the molecular and basic moiety ions and fragments of the antibiotic structure was derived. Specific peaks of the ions providing mass spectrometric identification of the antibiotic in various natural mixtures were detected.

[The antibiotic galtamycin. The structure of galtamycinone]. / Antibiotik galtamitsin. Stroenie galtamitsinona.

The structure of galtamycinone, a chromophore moiety of galtamycin, a new antitumor antibiotic was determined by spectral analysis, i.e. UV, NMR and mass spectrometry. Galtamycinone was shown to be 1,4,6-trioxy-10 [4 (e), 5 (e)-dioxy-6 (e)-methyl-tetrahydropyran-2(e)-yl]-8-methyl tetracendion-5,12. It represents a new type of anthracycline chromophores containing a C-glycoside bond in its molecule.