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1.
Nanotoxicology ; 18(2): 181-213, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38487921

RESUMO

The review systematizes data on the wide possibilities of practical application of carbon nanostructures. Much attention is paid to the use of carbon nanomaterials in medicine for the visualization of tumors during surgical interventions, in the creation of cosmetics, as well as in agriculture in the creation of fertilizers. Additionally, we demonstrate trends in research in the field of carbon nanomaterials with a view to elaborating targeted drug delivery systems. We also show the creation of nanosized medicinal substances and diagnostic systems, and the production of new biomaterials. A separate section is devoted to the difficulties in studying carbon nanomaterials. The review is intended for a wide range of readers, as well as for experts in the field of nanotechnology and nanomedicine.


Assuntos
Carbono , Nanoestruturas , Carbono/química , Nanoestruturas/química , Humanos , Animais , Nanomedicina/métodos , Sistemas de Liberação de Medicamentos , Materiais Biocompatíveis/química
2.
Nanomedicine ; 53: 102698, 2023 09.
Artigo em Inglês | MEDLINE | ID: mdl-37507062

RESUMO

The work aimed to investigate the biocompatibility and biological activity of the water-soluble fullerene adduct C60-Arg. It was found that the material is haemocompatible, is not cyto- and genotoxic, possesses pronounced antioxidant activity. Additionally, this paper outlines the direction of application of water-soluble fullerene adducts in the creation of neuroprotectors. It has been suggested that a putative mechanism of the protective action of the C60-Arg adduct is associated with its antioxidant properties, the ability to penetrate the blood-brain barrier, and release nitrogen monoxide as a result of the catabolism of L-arginine residues, which promote vascular relaxation. The action of the C60-Arg adduct was compared with the action of such an antioxidant as Edaravone, which is approved in Japan for the treatment of ischemic and haemorrhagic strokes.


Assuntos
Fulerenos , AVC Isquêmico , Nanoestruturas , Acidente Vascular Cerebral , Humanos , Antioxidantes/farmacologia , Antioxidantes/uso terapêutico , Fulerenos/farmacologia , Fulerenos/uso terapêutico , Fulerenos/química , Água , Acidente Vascular Cerebral/tratamento farmacológico , Isquemia , Arginina/uso terapêutico
3.
Biochim Biophys Acta Gen Subj ; 1867(9): 130384, 2023 09.
Artigo em Inglês | MEDLINE | ID: mdl-37209777

RESUMO

We report the synthesis of covalent conjugates of nanodiamonds with doxorubicin and a cytostatic drug from the class of 1,3,5-triazines. The obtained conjugates were identified using a number of physicochemical methods (IR-spectroscopy, NMR-spectroscopy, XRD, XPS, TEM). As a result of our study, it was found that ND-СONH-Dox and ND-COO-Diox showed good hemocompatibility, since they did not affect plasma coagulation hemostasis, platelet functional activity, and erythrocyte membrane. The ND-COO-Diox conjugates are also capable of binding to human serum albumin due to the presence of ND in their composition. In the study of the cytotoxic properties of ND-СONH-Dox and ND-COO-Diox in the T98G glioblastoma cell line, indicating that ND-СONH-Dox and ND-COO-Diox demonstrate greater cytotoxicity at lower concentrations of Dox and Diox in the composition of the conjugates compared to individual drugs; the cytotoxic effect of ND-COO-Diox was statistically significantly higher than that of ND-СONH-Dox at all concentrations studied. Greater cytotoxicity at lower concentrations of Dox and Diox in the composition of conjugates compared to individual cytostatics makes it promising to further study the specific antitumor activity and acute toxicity of these conjugates in models of glioblastoma in vivo. Our results demonstrated that ND-СONH-Dox and ND-COO-Diox enter HeLa cells predominantly via a nonspecific actin-dependent mechanism, while for ND-СONH-Dox a clathrin-dependent endocytosis pathway. All data obtained provide that the synthesized nanomaterials show a potential application as the agents for intertumoral administration.


Assuntos
Citostáticos , Glioblastoma , Nanodiamantes , Humanos , Nanodiamantes/química , Células HeLa , Doxorrubicina/química
4.
Biochem Biophys Res Commun ; 636(Pt 1): 50-56, 2022 12 25.
Artigo em Inglês | MEDLINE | ID: mdl-36332482

RESUMO

The aim of this work is to synthesise and study the biocompatibility and biological activity of the C70 fullerene adduct with l-threonine (C70-Thr). The obtained adduct was identified using a complex of physicochemical methods, namely, 13C NMR spectroscopy, IR spectroscopy, thermogravimetric analysis, electron spectroscopy, elemental analysis, and high-performance liquid chromatography. The study of biocompatibility and biological activity of the C70-Thr adduct included the study of haemocompatibility (haemolysis, platelet aggregation, plasma coagulation haemostasis, binding to human serum albumin, esterase activity), antiradical activity, cytotoxicity, cell proliferation, and interaction with DNA (determination of the DNA binding constant and genotoxicity).


Assuntos
Fulerenos , Humanos , Fulerenos/farmacologia , Treonina , Espectroscopia de Ressonância Magnética , Cromatografia Líquida de Alta Pressão
5.
Sci Rep ; 11(1): 8362, 2021 04 16.
Artigo em Inglês | MEDLINE | ID: mdl-33863918

RESUMO

The article is devoted to the study of the pharmacokinetics of fullerene C60 in oil and micellar forms, analysis of its content in blood, liver, lungs, kidneys, heart, brain, adrenal glands, thymus, testicles, and spleen. The highest accumulation of C60 was found in the liver and adrenal glands. As a result of the studies carried out, it was shown that the bioavailability of C60 in the micellar form is higher than that in an oil solution.


Assuntos
Antioxidantes , Fulerenos/metabolismo , Micelas , Óleos , Oxigênio/metabolismo , Animais , Fulerenos/administração & dosagem , Fulerenos/química , Fulerenos/farmacologia , Interações Hidrofóbicas e Hidrofílicas , Camundongos , Estrutura Molecular , Ratos , Ratos Wistar , Soluções , Distribuição Tecidual
6.
J Biotechnol ; 331: 83-98, 2021 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-33727085

RESUMO

Silica is silicon dioxide, which, depending on the production method, can exist in various amorphous forms with varying specific surface area, particle size, pore volume and size, and, as a result, with different physicochemical and sorption characteristics. The presence of silanol groups on the surface of silicas provides the possibility of its further functionalisation. In addition, the developed specific surface of Aerosil allows to obtain composites with a high content of biologically active substances. In this work, we studied the biocompatibility of a composite based on Aerosil 380 and carboxylated fullerene C60[C(COOH)2]3, namely: haemolysis (spontaneous and photoinduced), platelet aggregation, binding to HSA, cyto- and genotoxicity, antiradical activity. Interest in the creation of this nanomaterial is due to the fact that carboxylated fullerenes have potential applications in various fields of biomedicine, including the ability to bind reactive oxygen species, inhibition of tumour development, inactivation of viruses and bacteria. The obtained composite can be used for the immobilisation of various drugs and the further development of drugs for theranostics.


Assuntos
Fulerenos , Nanocompostos , Ácidos Carboxílicos , Espécies Reativas de Oxigênio , Dióxido de Silício
7.
Colloids Surf B Biointerfaces ; 196: 111338, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32882599

RESUMO

Amino acid adducts of light fullerenes have a potential of application in a variety of fields of biomedicine, that is reactive oxygen species scavenging activity, anticancer activity, viruses and bacteria inactivation etc. In this work, the water-soluble C60 fullerene derivative with l-hydroxyproline (C60(C5H9NO3)2, C60-Hyp) was studied. Extensive biomedical investigation of this compound, namely, antiradical activity in the reaction with stable diphenylpicrylhydrazyl radical, the binding to human serum albumin, photodynamic properties, cytotoxicity in glioblastoma A172 and lung carcinoma A549 cell lines, erythrocytes haemolysis, platelet aggregation, genotoxicity on human peripheral blood mononuclear cells was conducted. Moreover, the dynamic and structural characteristics of C60-Hyp-H2O binary system were obtained using molecular dynamic (MD) method, and size distribution along with ζ-potentials of C60-Hyp associates was measured.


Assuntos
Fulerenos , Água , Fulerenos/farmacologia , Humanos , Leucócitos Mononucleares , Simulação de Dinâmica Molecular , Espécies Reativas de Oxigênio
8.
Nanomedicine ; 29: 102284, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32781136

RESUMO

This article presents data on the synthesis, identification, computer simulation and biocompatibility of graphene oxide (GO) functionalized with L-cysteine (GFC). It was determined that GO reacts with L-cysteine in two different ways: in an alkaline medium, L-cysteine reduces functional groups on the surface and at the boundaries of GO; with heating and the use of thionyl chloride, L-cysteine covalently attaches to GO through carboxylic groups only at the boundaries. The identification of GO, reduced graphene oxide and GFC was performed using various physicochemical methods, including infrared spectroscopy, Raman spectroscopy, X-ray diffraction, X-ray photoelectron spectroscopy, thermogravimetric analysis, scanning electron microscopy and high-resolution transmission electron microscopy. Biocompatibility experiments included erythrocyte hemolysis, platelet aggregation, photodynamic and antiradical activity, binding to human serum albumin, and geno- and cytotoxicity studies. Applying density functional theory and molecular dynamics allowed us to obtain the structural and dynamic characteristics of a GFC-water binary system.


Assuntos
Materiais Biocompatíveis/química , Cisteína/química , Eritrócitos/efeitos dos fármacos , Grafite/química , Materiais Biocompatíveis/síntese química , Materiais Biocompatíveis/farmacologia , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Cisteína/síntese química , Cisteína/farmacologia , Grafite/síntese química , Grafite/farmacologia , Humanos , Microscopia Eletrônica de Varredura , Análise Espectral Raman , Óxidos de Enxofre/química , Óxidos de Enxofre/farmacologia
9.
Toxicol In Vitro ; 62: 104683, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31639450

RESUMO

One of the most studied fullerene members, C60, has a potential of application in various fields of biomedicine including reactive oxygen species (ROS) scavenging activity, inhibiting of tumours development, inactivating of viruses and bacteria, as well as elaboration of diagnostic and targeted drug delivery tools. However, the hydrophobicity of this molecule impedes its practical use, therefore the actuality of the research devoted to functionalisation of fullerenes leading to amphiphilic derivatives remains important. In this work, the water-soluble carboxylated fullerene derivative C60[C(COOH)2]3 was studied. Extensive biomedical investigation of this compound, namely, the binding with human serum albumin (HSA), radical scavenging activity in the reaction with diphenylpicrylhydrazyl (DPPH) radical, photodynamic properties, cytotoxicity in human embryonic kidney (HEK293) cell line, erythrocytes' haemolysis, platelet aggregation, and genotoxicity in human peripheral mononuclear cells (PBMC) was conducted. Moreover, the dynamic and structural characteristics of C60[C(COOH)2]3-H2O binary system were obtained using molecular dynamic (MD) method, and size distribution of C60[C(COOH)2]3 associates was measured.


Assuntos
Fulerenos/química , Fulerenos/toxicidade , Adulto , Compostos de Bifenilo/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Simulação por Computador , Feminino , Sequestradores de Radicais Livres/farmacologia , Células HEK293 , Humanos , Masculino , Simulação de Dinâmica Molecular , Mutagênicos/toxicidade , Picratos/toxicidade , Agregação Plaquetária/efeitos dos fármacos , Ligação Proteica , Solubilidade , Água
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