Influence of an educational intervention on pre-allied health students' attitudes toward older adults.

The purpose of this study was to determine the influence of an educational intervention on the knowledge and attitudes of pre-allied health students toward older adults. The subjects were 41 pre-allied health students assigned to an intervention (n = 19) or comparison group (n = 22). The intervention group participated in a six-hour multi-modal workshop consisting of didactic lecture, group activities, and simulation activities. A posttest only quasi-experimental design over time was used. Palmore's FACTS on Aging Quiz and Kogan's Old People Scale were used to measure knowledge and attitude towards older adults one week and 10 weeks following the workshop. The intervention significantly improved knowledge in the short term (p = 0.031). No significant difference was noted between the two groups on the attitudinal surveys.

Characterization of the targeting signal of the Arabidopsis 22-kD integral peroxisomal membrane protein.

Using a combination of in vivo and in vitro assays, we characterized the sorting pathway and molecular targeting signal for the Arabidopsis 22-kD peroxisome membrane protein (PMP22), an integral component of the membrane of all peroxisomes in the mature plant. We show that nascent PMP22 is sorted directly from the cytosol to peroxisomes and that it is inserted into the peroxisomal boundary membrane with its N- and C-termini facing the cytosol. This direct sorting of PMP22 to peroxisomes contrasts with the indirect sorting reported previously for cottonseed (Gossypium hirsutum) ascorbate peroxidase, an integral PMP that sorts to peroxisomes via a subdomain of the endoplasmic reticulum. Thus, at least two different sorting pathways for PMPs exist in plant cells. At least four distinct regions within the N-terminal one-half of PMP22, including a positively charged domain present in most peroxisomal integral membrane-destined proteins, functions in a cooperative manner in efficient peroxisomal targeting and insertion. In addition, targeting with high fidelity to peroxisomes requires all four membrane-spanning domains in PMP22. Together, these results illustrate that the PMP22 membrane peroxisomal targeting signal is complex and that different elements within the signal may be responsible for mediating unique aspects of PMP22 biogenesis, including maintaining the solubility before membrane insertion, targeting to peroxisomes, and ensuring proper assembly in the peroxisomal boundary membrane.