[Features of the metabolic composition of spermal plasma in different morphofunctional pathologies of the ejaculate.]

As part of the work, an assessment was made of the state of the metabolic characteristics of sperm plasma in various morphofunctional ejaculate pathologies. Physico-chemical, metabolic and morphological parameters of the ejaculate were evaluated. The diagnostic protocol included a standard sperm analysis with classification of ejaculate parameters and determination of biochemical parameters of sperm plasma on an automatic biochemical analyzer. The study of the qualitative and quantitative characteristics of metabolic parameters. The total number of surveyed was XX people. After studying the morphological features of the cell composition, the obtained samples were divided into four groups: samples with normospermia, samples with oligoasthenoteratozoospermia, samples with azoospermia, and samples with cryptozoospermia. In each group, the parameters of protein, carbohydrate, lipid and mineral metabolism were studied. As a result, it was found that with the progression of pathology, the catabolic mechanism of energy consumption begins to prevail, due to a decrease in the concentration in the sperm plasma of protein components, transaminases, as well as the growth of non-protein nitrogenous inclusions. It is noted that the growth of glucose, despite the inhibition of transmembrane routes of intake by reducing the concentration of alkaline phosphatase and lipid-transport systems, is associated with the forced inclusion of insulating mechanisms for the penetration of glucose into the sperm plasma from the blood through the hemato-sperm barrier. At the same time, an increase in the content of lipids in the sperm plasma, as well as the growth of creatinine in the initial stages of the development of pathology, can be characterized by a compensatory response aimed at maintaining the viability of minimal amounts of spermatozoa. Well, the disorder of mineral metabolism in general characterizes the violation of metabolic processes in all forms of ejaculate pathology. Identified features of the metabolism in the sperm plasma may further allow to identify more informative laboratory markers of male infertility.

[Coagulation test features depending on the AB0-blood groups system antigenic composition.]

The maintenance of normal blood flow through the vessels is the result of the coordinated work of the coagulation and anticoagulation systems of our body. The balance of this system depends on many factors, including endothelial, humoral, platelet ones, however, we still lack knowledge about the effect of antigenic determinants on the state of the hemostatic system. This study is devoted to assessing the effect of the presence and absence of antigens on the AB0 system, presented on erythrocyte and platelet membranes, on hemostatic parameters. The study was conducted in the Clinics of Samara State Medical University and consisted of127 clinically healthy individuals who underwent a general analysis and biochemical blood analysis, 52 people with the most stable indicators of cell composition and metabolic profile were selected for a coagulation test, including determination of the activity of coagulation factors and routine tests. A significant decrease in the activity of the VIII and VII coagulation factors was revealed, as well as an increase in the prothrombin time in patients with 0 (I) blood group compared to the "antigenic" blood groups. The presence of biological variation for indicators of external and internal coagulation paths was noted, depending on the group of blood belonging to the AB0 system. The findings suggest that there is an increased susceptibility to bleeding in patients with 0 (I) blood groups due to the absence of antigenic determinants on the cell membrane, while for "antigenic" blood groups, on the contrary, there is a susceptibility to thrombosis due to increased activity of the components of the coagulation system.