[The interrelation of the expression of HLA class I and II proteins and of the costimulatory proteins CD11a/CD18 and CD16 with the lytic activity of resting and activated natural killer lymphocytes]. / Vzaimosviaz' ékspressii HLA-belkov I i II klassov i kostimuliatornykh belkov CD11a/CD18 i CD16 s liticheskoi aktivnost'iu pokoiashchikhsia i aktivirovannykh estestvennykh killernykh limfotsitov.

It has been presumed that: 1) rheumatoid arthritis (RA) is a genuine model of activation of the peripheral natural killers (NK); 2) methods of NK separation can cause their activation, and therefore only peripheral mononuclear cells were used. The aim of this research was to study the role of cytolysis of HLA-I, HLA-DR, and costimulatory proteins CD11a and CD16 expressed on resting and activated peripheral NK cells. A total of 74 healthy volunteers (HV) and 74 RA patients were compared before corticosteroid or gold treatment. Two-colour immunofluorescence and cytofluorimetric analysis, cytotoxic test against the target K 562 cells (class I negative) before and after incubation of PMC with monoclonal antibodies (MoAB) to HLA-I (E3D8), HLA-DR (ICO-1), CD11 (ICO-11), PXx63 Ag8.653 as control, and also incubation with recombinant alpha 2-IFN were used. VEP-13 MoAB was used for CD16 determination. Compared with HV PMC, the RA PMC showed a statistically significant increase of activated NK lymphocytes (CD11a+DR+ and CD16+DR+ by 2.03 and 1.96 times, resp.), and increased level of expression of HLA-I, HLA-DR, and CD16 on CD11a+ lymphocytes, resulting in significantly diminished cytolytic activity (CA) by 1.9 times. A short-term incubation with alpha 2-IFN (100 U/ml, 1 h) increased the level of expression of HLA- and costimulatory proteins and the CA of the HV PMC, while the RA PMC did not react. HV peripheral NK are concluded to be mainly resting cells, and the RA peripheral NK are mainly the activated ones. Only using the level of expression of HLA-DR on CD11a lymphocytes, as a ranking criterion, allowed to reveal two types of correlation between the CA and immunocytological parameters of PMC. The first type: in HV, the CA correlated positively (P < 0.025) with the percentage of CD11a+CD16+, and with levels of expression of HLA-I (P < 0.025) and CD16 (P < 0.025) expressed on CD11a lymphocytes (5-9.5 MFI DR--the mean fluorescence intensity of HLA-DR). The second type: the CA positively correlated (P < 0.05) with the percentage of CD11a+CD16-, and with levels of expression of HLA-DR (P < 0.05) and CD11a (P < 0.05) expressed on CD11a lymphocytes (12.4-16 MFI DR). In RA patients with a "normal" density expression of HLA-DR on CD11a lymphocytes (up to 14 MFI) there exists a mixed type: the CA correlated positively (P < 0.05) with the percentage of CD11a+CD16+, and with levels of expression of HLA-DR (P < 0.05) and CD11a (P < 0.05) expressed on CD11a lymphocytes. In RA patients with a very high density expression of HLA-DR on CD11a lymphocytes (VH-DR group, 18-26 MFI) the CA correlated negatively with the percentage of CD11a+CD1 6+ (P < 0.05), and with levels of expression of HLA-DR (P < 0.025) and CD11a (P < 0.025) expressed on CD11a lymphocytes. The anti-HLA-I,-DR, -CD11a MoABs strongly inhibit the Ca in HV- and RA-PMC. However, in the VH-DR group the anti-DR MoAB stimulates the CA from 27.06 +/- 10.25 up to 41.69 +/- 14.23%. Thus, activated peripheral CD11a+CD16+ lymphocytes suppress the CA of other NK.

[The effect of plasmin and its complexes with alpha 2-antiplasmin and alpha 2-macroglobulin on secretion of proteinase and plasminogen inhibitors from peripheral mononuclear cells]. / Vliianie plazmina i ego kompleksov s alpha 2-antiplazminom i alpha 2-makroglobulinom na sekretsiiu ingibitorov proteinaz i plazminogena perifericheskimi mononuklearnymi kletkami.

Secretion of proteinase inhibitors alpha2-macroglobulin, alpha1-proteinase inhibitor, inter-alpha-trypsin inhibitor and plasminogen was distinctly altered in mononuclear cell from peripheric blood of volunteers if these cells were preincubated simultaneously with alpha2-macroglobulin and alpha2-antiplasmin modified by plasmin. The complex plasmin-alpha2-macroglobulin was shown to stimulate most effectively the alpha2-macroglobulin and alpha1-proteinase inhibitor secretion. Besides, plasmin-alpha2-macroglobulin complex inhibited the inter-alpha-trypsin inhibitor secretion, whereas the complex plasmin-alpha2-antiplasmin--stimulated. Both these complexes augmented the rate of plasminogen synthesis in the mononuclear cells.

[The effect of plasmin-modified proteinase inhibitors on macroglobulin production by human blood mononuclear cells in culture]. / Vliianie modifitsirovannykh plazminom ingibitorov proteinaz na produktsiiu makroglobulinov mononuklearnymi kletkami krovi cheloveka v kul'ture.

A study was made of the influence of plasmin (PL) complexes with alpha 2-macroglobulin (MG) and alpha 2-antiplasmin on secretion of MG, pregnancy-associated alpha 2-glycoprotein (PAG), and pregnancy-associated plasma protein A (PAPP-A) by mononuclear cells (MC), obtained from human peripheral blood. It has been shown that incubation of the MG-PL complexes with MC resulted in the increase in concentrations of all these macroglobulins (MG, PAG, and PAPP-A) in supernatants of cultured MC. We revealed the same effects of AP-PL with regard to the secretion of PZP and PAPP-A. However, this complex was found to suppress MG production by cultured MC. The effects found were dose dependent and some of them differed greatly in men and women, resp.

[The use of colloidal gold for the immunocytochemical analysis of subpopulations of immunocompetent cells]. / Ispol'zovanie kolloidnogo zolota dlia immunotsitokhimicheskogo analiza subpopuliatsii immunokompetentnykh kletok.

Immune colloid gold is suggested to be used for assessment of human peripheral blood lymphocyte subpopulations. The method is simple, highly sensitive, and compatible with immunofluorescent technique. Double label permitted detection of beta 2-macroglobulin-positive cells within every lymphocyte subpopulations.

[Time dependence of the shedding of class I and II HLA antigens. Interrelation of the fluctuations of HLA-antigen shedding and 51Cr-labeled macromolecules]. / Zavisimost' shedinga HLA-antigenov I i II klassov ot vremeni. Sviaz' kolebanii shedinga HLA-antigenov i mechennykh 51Cr makromolekul.

Soluble HLA-A, -B and -DR antigens were determined every 10 min with a rate nephelometry method in supernatants of human mononuclear cells cultured for 3 hrs. The time-dependent oscillations in the quantity of soluble HLA antigens with ultradian (circadian) rhythm characteristics were found. Quantity oscillations of HLA-A antigens were synphase to HLA-B ones and antiphase to HLA-DR antigens. Amplitude characteristics were found to depend on the temperature and cytoskeleton activity.

[Effect of thymosin on human lymphocyte subpopulations in vitro]. / Vliianie timozina na subpopuliatsii limfotsitov cheloveka in vitro.

The incubation of healthy donors peripheral blood lymphocytes with thymosin was shown to increase the content of high avide "active" lymphocytes bearing receptors for SRBC, lymphocytes bearing receptors for C(2) component of complement and for Fc-portion of immunoglobulins. The proportion of cap-forming Ig-positive lymphocytes was shown to increase too. The role of T and B lymphocyte subpopulations and their precursors as thymosin action target cells is discussed.