Assuntos
Esterases/metabolismo , Glicosídeo Hidrolases/metabolismo , Fungos Mitospóricos/enzimologia , Doenças das Plantas , Poaceae/enzimologia , Sulfato de Amônio , Cloreto de Cálcio , Celulose/metabolismo , Precipitação Química , Cromatografia em Camada Fina , Filtração , Concentração de Íons de Hidrogênio , Pectinas/metabolismo , Extratos Vegetais , Espectrofotometria , Ácidos Urônicos , Verduras , ViscosidadeRESUMO
'Wando' pea is susceptible to Ditylenchus dipsaci from Raleigh, N. C. (RNC) but resistant to the same species from Waynesville, N. C. (WNC). Homogenates of RNC and WNC were analyzed for pectolytic and cellulolytic enzyme activity; both had high C(x) activity with WNC two to three times more active than RNC. Polymethylglacturonase activity was three to five times higher in RNC, but polygalacturonase was up to 100 times higher in WNC. Polygalacturonate-trans-eliminase was not detected although a Ca(++)-stimulated pectin methyl-trans-eliminase was present. Enzyme analyses of healthy and infected pea tissue showed only slight enzyme activity unrelated to that in nematode homogenates. No correlation between enzyme activity and the differing pathogenicities could be detected.
