RESUMO
Specific features of hantavirus infection in bank vole (Clethrionomys glareolus) were studied in the endemic area of hemorrhagic fever with renal syndrome (HFRS) in the foothills of the Ural mountains, using long-term observations on living animals by the capture-mark-recapture (CMR) method. The results demonstrated that the infection naturally circulating in the voles is chronic (lasting for up to 15 months) and asymptomatic, with a peak of Puumala virus accumulation and release from the organism during the first month after infection. It was shown that the bank vole population includes young animals with maternal immunity, which remain resistant to the Puumala virus infection for 3-3.5 months. The infection rate in voles depended on the age and sexual maturity of animals. The greatest proportion of seropositive animals was observed among overwintered males. Seroconversion in voles was more frequent during the period of high reproductive activity.
Assuntos
Arvicolinae/virologia , Infecções por Hantavirus/veterinária , Orthohantavírus/fisiologia , Doenças dos Roedores/virologia , Envelhecimento , Animais , Anticorpos Antivirais/sangue , Antígenos Virais/análise , Arvicolinae/fisiologia , Suscetibilidade a Doenças , Feminino , Orthohantavírus/imunologia , Orthohantavírus/isolamento & purificação , Infecções por Hantavirus/imunologia , Infecções por Hantavirus/transmissão , Infecções por Hantavirus/virologia , Imunidade Materno-Adquirida , Masculino , Reprodução , Doenças dos Roedores/imunologia , Doenças dos Roedores/transmissão , Estações do Ano , Caracteres SexuaisAssuntos
Surtos de Doenças , Febre Hemorrágica com Síndrome Renal/epidemiologia , Febre Hemorrágica com Síndrome Renal/virologia , Orthohantavírus/classificação , Adulto , Animais , Feminino , Orthohantavírus/isolamento & purificação , Humanos , Masculino , Pessoa de Meia-Idade , Muridae/virologia , Federação Russa/epidemiologiaRESUMO
A novel hantavirus has been discovered in European common voles, Microtus arvalis and Microtus rossiaemeridionalis. According to sequencing data for the genomic RNA S segment and nucleocapsid protein and data obtained by immunoblotting with a panel of monoclonal antibodies, the virus, designated Tula virus, is a distinct novel member of the genus Hantavirus. Phylogenetic analyses of Tula virus indicate that it is most closely related to Prospect Hill, Puumala, and Muerto Canyon viruses. The results support the view that the evolution of hantaviruses follows that of their primary carriers. Comparison of strains circulating within a local rodent population revealed a genetic drift via accumulation of base substitutions and deletions or insertions. The Tula virus population from individual animals is represented by quasispecies, indicating the potential for rapid evolution of the agent.
Assuntos
Arvicolinae/virologia , Orthohantavírus/genética , Orthohantavírus/isolamento & purificação , Filogenia , Sequência de Aminoácidos , Animais , Sequência de Bases , Capsídeo/biossíntese , Capsídeo/isolamento & purificação , Sequência Conservada , Primers do DNA , Variação Genética , Genoma Viral , Orthohantavírus/classificação , Immunoblotting , Dados de Sequência Molecular , Mutação , Fases de Leitura Aberta , Reação em Cadeia da Polimerase , RNA Viral/biossíntese , RNA Viral/isolamento & purificação , Homologia de Sequência de Aminoácidos , Proteínas do Core Viral/biossíntese , Proteínas do Core Viral/isolamento & purificaçãoRESUMO
An immunoglobulin G avidity assay was used to determine recent and past hantavirus infection in bank voles (Clethrionomys glareolus). Sera of experimentally infected bank voles were studied at different time intervals. The avidity of specific IgG increased over time after infection. This experimental data were used to estimate the time after onset of hantavirus infection in naturally infected bank voles caught in an endemic area. The possibility to discriminate between recently infected animals and animals infected some time ago is important since the proportion of recently infected bank voles represents the intensity of the epizootic which in turn correlates to the risk of humans to contract the disease.
