Paracoccus aeridis sp. nov., an indole-producing bacterium isolated from the rhizosphere of an orchid, Aerides maculosa.

A Gram-stain-negative, non-motile, coccoid-shaped, catalase- and oxidase-positive, non-denitrifying, neutrophilic bacterium designated as strain JC501T was isolated from an epiphytic rhizosphere of an orchid, Aerides maculosa, growing in the Western Ghats of India. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that strain JC501T belonged to the genus Paracoccus and had the highest levels of sequence identity with Paracoccus marinus KKL-A5T (98.9 %), Paracoccus contaminans WPAn02T (97.3 %) and other members of the genus Paracoccus (<97.3 %). Strain JC501T produced indole-3 acetic acid and other indole derivatives from tryptophan. The dominant respiratory quinone was Q-10 and the major fatty acid was C18 : 1ω7c/C18 : 1ω6c, with significant quantities of C18 : 1ω9c, C17 : 0 and C16 : 0. The polar lipids of strain JC501T comprised phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, an unidentified glycolipid, two unidentified aminolipids, two unidentified lipids and four unidentified phospholipids. The genome of strain JC501T was 3.3 Mbp with G+C content of 69.4 mol%. For the resolution of the phylogenetic congruence of the novel strain, the phylogeny was also reconstructed with the sequences of eight housekeeping genes. Based on the results of phylogenetic analyses, low (<85.9 %) average nucleotide identity, digital DNA-DNA hybridization (<29.8 %), chemotaxonomic analysis and physiological properties, strain JC501T could not be classified into any of the recognized species of the genus Paracoccus. Strain JC501T represents a novel species, for which the name Paracoccus aeridis sp. nov. is proposed. The type strain is JC501T (=LMG 30532T=NBRC 113644T).

Chryseobacterium candidae sp. nov., isolated from a yeast (Candida tropicalis).

A Gram-stain-negative, rod shaped, non-motile, aerobic bacterium (strain JC507T) was isolated from a yeast (Candida tropicalis JY101). Strain JC507T was oxidase- and catalase-positive. Complete 16S rRNA gene sequence comparison data indicated that strain JC507T was a member of the genus Chryseobacterium and was closely related to Chryseobacterium indologenes NBRC 14944T (98.7 %), followed by Chryseobacterium arthrosphaerae CC-VM-7T (98.6 %), Chryseobacterium gleum ATCC 35910T (98.5 %) and less than 98.5 % to other species of the genus Chryseobacterium.The genomic DNA G+C content of strain JC507T was 36.0 mol%. Strain JC507T had phosphatidylethanolamine, four unidentified amino lipids and four unidentified lipids. MK-6 was the only respiratory quinone. The major fatty acids (>10 %) were anteiso-C11 : 0, iso-C15 : 0 and iso-C17 : 03OH. The average nucleotide identity and in silico DNA-DNA hybridization values between strain JC507T and C. indologenes NBRC 14944T, C. arthrosphaerae CC-VM-7T and C. gleum ATCC 35910T were 80.2, 83.0 and 87.0 % and 24, 26.7 and 32.7 %, respectively. The results of phenotypic, phylogenetic and chemotaxonomic analyses support the inclusion of strain JC507T as a representative of a new species of the genus Chryseobacterium, for which the name Chryseobacteriumcandidae sp. nov. is proposed. The type strain is JC507T (=KCTC 52928T=MCC 4072T=NBRC 113872T).