RESUMO
A lambda L 47.1 clone library was constructed from BamHI digests of cellular cat DNA and was screened with cDNA probes specific for the 3'-terminus of the replication competent RD-114 virus. The first analysis of one clone (lambda-ECE 1) shows that it consists at least of a 3'-LTR and a 5' adjacent env gene. The LTR nucleotide sequences of the RD-114 cDNA clones and ECE 1 are identical within the R region and unrelated to FeLV. Otherwise, the region upstream from the 3'-LTR of ECE 1 shows homology to the transmembrane protein encoding gene of FeLV, but not to RD-114. These results imply that ECE 1 is a recombinant between an RD-114-like 3'-LTR and a FeLV-related env portion.
Assuntos
Gatos/microbiologia , Retroviridae/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , DNA/genética , Vírus da Leucemia Felina/genética , Dados de Sequência Molecular , Recombinação Genética , Retroviridae/isolamento & purificação , Homologia de Sequência do Ácido NucleicoRESUMO
Occurrence of thymus cell tumours was followed in cattle with enzootic leukosis using a thymus-specific antiserum. Among 32 tumorous lymph nodes investigated, one could be identified as a thymus cell tumour. In the DNA extract from this tumorous lymph node BLV-specific sequences have been demonstrated. This finding disproved the hitherto assumption that BLV-induced lymph node tumours of cattle were derived exclusively from B-lymphocytes.
Assuntos
Antígenos de Neoplasias/análise , Leucemia Experimental/patologia , Linfonodos/imunologia , Glicoproteínas de Membrana , Linfócitos T , Animais , Bovinos , Membrana Celular/imunologia , DNA Viral/análise , Ensaio de Imunoadsorção Enzimática , Imunoglobulina G/análise , Vírus da Leucemia Bovina/genética , Vírus da Leucemia Bovina/imunologia , Leucemia Experimental/imunologia , Leucemia Experimental/microbiologia , Linfonodos/ultraestrutura , Hibridização de Ácido Nucleico , Linfócitos T/imunologiaRESUMO
Over 90% of the total viral information together with the adjacent 5' cellular sequences were cloned in the lambdoid spi selection vector lambda-2558 by taking advantage of the unique EcoRI restriction site very close to the 3' long terminal repeat. Of the fifteen isolated recombinant phages with viral inserts, one has been propagated and its DNA isolated and subjected to preliminary restriction endonuclease analysis. The proviral insert has been found to be almost identical to the unintegrated proviral DNA reported by Kashmiri et al. (1984).
Assuntos
DNA Viral/genética , Vírus da Leucemia Bovina/genética , Retroviridae/genética , Animais , Bacteriófago lambda , Linhagem Celular , Clonagem Molecular , Enzimas de Restrição do DNA , Escherichia coli/genética , Vetores Genéticos , Rim , Ovinos , Cultura de VírusRESUMO
Bovine leukaemia virus (BVL) in circulating lymphatic cells of the blood is repressed and has to be activated for its expression. After in situ hybridization of BLV-3HcDNA with a specific activity of 3.9 X 10 dpm/micrograms to noncultivated blood cells from 5 leukotic cattle, in 4 of these animals from 0.96% to 1.5% lymphocytes were found to contain BLV-specific RNA sequences. Non-hybridized lymphocytes as well as calf thymus cells, used as controls, gave only 0.47% and 0.39% labelled cells, respectively. After short-term cultivation of white blood cells from these leukotic animals the percentage of labelled cells increased. During the short-term cultivation the number of hybridizing cells from one and the same animal and from all animal, when compared with each other, varied to a high extent. The highest values of BLV-RNA sequences-containing cells from all 5 animals at different cultivation times were within the range of from 2.4 to 16.2%.
