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1.
Nat Med ; 2024 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-38816609

RESUMO

Accurately predicting functional outcomes for unresponsive patients with acute brain injury is a medical, scientific and ethical challenge. This prospective study assesses how a multimodal approach combining various numbers of behavioral, neuroimaging and electrophysiological markers affects the performance of outcome predictions. We analyzed data from 349 patients admitted to a tertiary neurointensive care unit between 2009 and 2021, categorizing prognoses as good, uncertain or poor, and compared these predictions with observed outcomes using the Glasgow Outcome Scale-Extended (GOS-E, levels ranging from 1 to 8, with higher levels indicating better outcomes). After excluding cases with life-sustaining therapy withdrawal to mitigate the self-fulfilling prophecy bias, our findings reveal that a good prognosis, compared with a poor or uncertain one, is associated with better one-year functional outcomes (common odds ratio (95% CI) for higher GOS-E: OR = 14.57 (5.70-40.32), P < 0.001; and 2.9 (1.56-5.45), P < 0.001, respectively). Moreover, increasing the number of assessment modalities decreased uncertainty (OR = 0.35 (0.21-0.59), P < 0.001) and improved prognostic accuracy (OR = 2.72 (1.18-6.47), P = 0.011). Our results underscore the value of multimodal assessment in refining neuroprognostic precision, thereby offering a robust foundation for clinical decision-making processes for acutely brain-injured patients. ClinicalTrials.gov registration: NCT04534777 .

3.
Rev Neurol (Paris) ; 178(1-2): 9-20, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34980510

RESUMO

Probing consciousness and cognitive abilities in non-communicating patients is one of the most challenging diagnostic issues. A fast growing medical and scientific literature explores the various facets of this challenge, often coined under the generic expression of 'Disorders of Consciousness' (DoC). Crucially, a set of independent converging results demonstrated both (1) the diagnostic and prognostic importance of this expertise, and (2) the need to combine behavioural measures with brain structure and activity data to improve diagnostic and prognostication accuracy as well as potential therapeutic intervention. Thus, probing consciousness in DoC patients appears as a crucial activity rich of human, medical, economic and ethical consequences, but this activity needs to be organized in order to offer this expertise to each concerned patient. More precisely, diagnosis of consciousness differs in difficulty across patients: while a minimal set of data can be sufficient to reach a confident result, some patients need a higher level of expertise that relies on additional behavioural and brain activity and brain structure measures. In order to enable this service on a systematic mode, we present two complementary proposals in the present article. First, we sketch a structuration of DoC expertise at a country-scale, namely France. More precisely, we suggest that a 2-tiers network composed of local (Tier-1) and regional (Tier-2) centers backed by distant electronic databases and algorithmic centers could optimally enable the systematic implementation of DoC expertise in France. Second, we propose to create a national common register of DoC patients in order to better monitor this activity, to improve its performance on the basis of nation-wide collected evidence, and to promote rational decision-making.


Assuntos
Transtornos da Consciência , Estado de Consciência , Encéfalo , Transtornos da Consciência/diagnóstico , Transtornos da Consciência/terapia , França , Humanos , Prognóstico
4.
Clin Neurophysiol ; 131(11): 2736-2765, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-32917521

RESUMO

The analysis of spontaneous EEG activity and evoked potentialsis a cornerstone of the instrumental evaluation of patients with disorders of consciousness (DoC). Thepast few years have witnessed an unprecedented surge in EEG-related research applied to the prediction and detection of recovery of consciousness after severe brain injury,opening up the prospect that new concepts and tools may be available at the bedside. This paper provides a comprehensive, critical overview of bothconsolidated and investigational electrophysiological techniquesfor the prognostic and diagnostic assessment of DoC.We describe conventional clinical EEG approaches, then focus on evoked and event-related potentials, and finally we analyze the potential of novel research findings. In doing so, we (i) draw a distinction between acute, prolonged and chronic phases of DoC, (ii) attempt to relate both clinical and research findings to the underlying neuronal processes and (iii) discuss technical and conceptual caveats.The primary aim of this narrative review is to bridge the gap between standard and emerging electrophysiological measures for the detection and prediction of recovery of consciousness. The ultimate scope is to provide a reference and common ground for academic researchers active in the field of neurophysiology and clinicians engaged in intensive care unit and rehabilitation.


Assuntos
Transtornos da Consciência/diagnóstico , Eletroencefalografia/métodos , Potenciais Evocados/fisiologia , Estado de Consciência/fisiologia , Transtornos da Consciência/fisiopatologia , Humanos , Prognóstico
5.
Eur J Neurol ; 27(5): 741-756, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32090418

RESUMO

BACKGROUND AND PURPOSE: Patients with acquired brain injury and acute or prolonged disorders of consciousness (DoC) are challenging. Evidence to support diagnostic decisions on coma and other DoC is limited but accumulating. This guideline provides the state-of-the-art evidence regarding the diagnosis of DoC, summarizing data from bedside examination techniques, functional neuroimaging and electroencephalography (EEG). METHODS: Sixteen members of the European Academy of Neurology (EAN) Scientific Panel on Coma and Chronic Disorders of Consciousness, representing 10 European countries, reviewed the scientific evidence for the evaluation of coma and other DoC using standard bibliographic measures. Recommendations followed the Grading of Recommendations Assessment, Development and Evaluation (GRADE) system. The guideline was endorsed by the EAN. RESULTS: Besides a comprehensive neurological examination, the following suggestions are made: probe for voluntary eye movements using a mirror; repeat clinical assessments in the subacute and chronic setting, using the Coma Recovery Scale - Revised; use the Full Outline of Unresponsiveness score instead of the Glasgow Coma Scale in the acute setting; obtain clinical standard EEG; search for sleep patterns on EEG, particularly rapid eye movement sleep and slow-wave sleep; and, whenever feasible, consider positron emission tomography, resting state functional magnetic resonance imaging (fMRI), active fMRI or EEG paradigms and quantitative analysis of high-density EEG to complement behavioral assessment in patients without command following at the bedside. CONCLUSIONS: Standardized clinical evaluation, EEG-based techniques and functional neuroimaging should be integrated for multimodal evaluation of patients with DoC. The state of consciousness should be classified according to the highest level revealed by any of these three approaches.


Assuntos
Coma/diagnóstico , Transtornos da Consciência/diagnóstico , Neurologia , Estado de Consciência , Eletroencefalografia , Europa (Continente) , Humanos , Sociedades Médicas
6.
Sci Adv ; 5(2): eaat7603, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30775433

RESUMO

Adopting the framework of brain dynamics as a cornerstone of human consciousness, we determined whether dynamic signal coordination provides specific and generalizable patterns pertaining to conscious and unconscious states after brain damage. A dynamic pattern of coordinated and anticoordinated functional magnetic resonance imaging signals characterized healthy individuals and minimally conscious patients. The brains of unresponsive patients showed primarily a pattern of low interareal phase coherence mainly mediated by structural connectivity, and had smaller chances to transition between patterns. The complex pattern was further corroborated in patients with covert cognition, who could perform neuroimaging mental imagery tasks, validating this pattern's implication in consciousness. Anesthesia increased the probability of the less complex pattern to equal levels, validating its implication in unconsciousness. Our results establish that consciousness rests on the brain's ability to sustain rich brain dynamics and pave the way for determining specific and generalizable fingerprints of conscious and unconscious states.


Assuntos
Encéfalo/fisiologia , Conectoma , Estado de Consciência , Vias Neurais , Adulto , Mapeamento Encefálico , Feminino , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Neuroimagem
7.
Br J Anaesth ; 121(6): 1290-1297, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30442256

RESUMO

BACKGROUND: Mismatch negativity (MMN) is the neurophysiological correlate of cognitive integration of novel stimuli. Although MMN is a well-established predictor of awakening in non-sedated comatose patients, its prognostic value in deeply sedated critically ill patients remains unknown. The aim of this prospective, observational pilot study was to investigate the prognostic value of MMN for subsequent awakening in deeply sedated critically ill patients. METHODS: MMN was recorded in 43 deeply sedated critically ill patients on Day 3 of ICU admission using a classical 'odd-ball' paradigm that delivers rare deviant sounds in a train of frequent standard sounds. Individual visual analyses and a group level analysis of recordings were performed. MMN amplitudes were then analysed according to the neurological status (awake vs not awake) at Day 28. RESULTS: Median (inter-quartile range) Richmond Assessment Sedation Scale (RASS) at the time of recording was -5 (range, from -5 to -4.5). Visual detection of MMN revealed a poor inter-rater agreement [kappa=0.17, 95% confidence interval (0.07-0.26)]. On Day 28, 30 (70%) patients had regained consciousness while 13 (30%) had not. Quantitative group level analysis revealed a significantly greater MMN amplitude for patients who awakened compared with those who had not [mean (standard deviation) = -0.65 (1.4) vs 0.08 (0.17) µV, respectively; P=0.003). CONCLUSIONS: MMN can be observed in deeply sedated critically ill patients and could help predict subsequent awakening. However, visual analysis alone is unreliable and should be systematically completed with individual level statistics.


Assuntos
Estado Terminal , Sedação Profunda , Vigília , Adulto , Idoso , Idoso de 80 Anos ou mais , Cognição , Estado de Consciência , Potenciais Evocados , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Projetos Piloto , Estudos Prospectivos
8.
Rev Neurol (Paris) ; 173(7-8): 521-528, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28843414

RESUMO

By looking for properties of consciousness, cognitive neuroscience studies have dramatically enlarged the scope of unconscious cognitive processing. This emerging knowledge inspired the development of new approaches allowing clinicians to probe and disentangle conscious from unconscious cognitive processes in non-communicating brain-injured patients both in terms of behaviour and brain activity. This information is extremely valuable in order to improve diagnosis and prognosis in such patients both at acute and chronic settings. Reciprocally, the growing observations coming from such patients suffering from disorders of consciousness provide valuable constraints to theoretical models of consciousness. In this review we chose to illustrate these recent developments by focusing on brain signals recorded with EEG at bedside in response to auditory stimuli. More precisely, we present the respective EEG markers of unconscious and conscious processing of two classes of auditory stimuli (sounds and words). We show that in both cases, conscious access to the corresponding representation (e.g.: auditory regularity and verbal semantic content) share a similar neural signature (P3b and P600/LPC) that can be distinguished from unconscious processing occurring during an earlier stage (MMN and N400). We propose a two-stage serial model of processing and discuss how unconscious and conscious signatures can be measured at bedside providing relevant informations for both diagnosis and prognosis of consciousness recovery. These two examples emphasize how fruitful can be the bidirectional approach exploring cognition in healthy subjects and in brain-damaged patients.


Assuntos
Cognição/fisiologia , Estado de Consciência/fisiologia , Potenciais Evocados/fisiologia , Inconsciência/fisiopatologia , Inconsciência/psicologia , Estimulação Acústica/psicologia , Eletroencefalografia , Humanos , Processos Mentais/fisiologia
9.
Neuroimage ; 83: 726-38, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23859924

RESUMO

Detecting residual consciousness in unresponsive patients is a major clinical concern and a challenge for theoretical neuroscience. To tackle this issue, we recently designed a paradigm that dissociates two electro-encephalographic (EEG) responses to auditory novelty. Whereas a local change in pitch automatically elicits a mismatch negativity (MMN), a change in global sound sequence leads to a late P300b response. The latter component is thought to be present only when subjects consciously perceive the global novelty. Unfortunately, it can be difficult to detect because individual variability is high, especially in clinical recordings. Here, we show that multivariate pattern classifiers can extract subject-specific EEG patterns and predict single-trial local or global novelty responses. We first validate our method with 38 high-density EEG, MEG and intracranial EEG recordings. We empirically demonstrate that our approach circumvents the issues associated with multiple comparisons and individual variability while improving the statistics. Moreover, we confirm in control subjects that local responses are robust to distraction whereas global responses depend on attention. We then investigate 104 vegetative state (VS), minimally conscious state (MCS) and conscious state (CS) patients recorded with high-density EEG. For the local response, the proportion of significant decoding scores (M=60%) does not vary with the state of consciousness. By contrast, for the global response, only 14% of the VS patients' EEG recordings presented a significant effect, compared to 31% in MCS patients' and 52% in CS patients'. In conclusion, single-trial multivariate decoding of novelty responses provides valuable information in non-communicating patients and paves the way towards real-time monitoring of the state of consciousness.


Assuntos
Transtornos da Consciência/fisiopatologia , Estado de Consciência/fisiologia , Processamento de Sinais Assistido por Computador , Estimulação Acústica , Adulto , Encéfalo/fisiologia , Eletroencefalografia , Feminino , Humanos , Magnetoencefalografia , Masculino , Pessoa de Meia-Idade , Adulto Jovem
10.
Arch Ital Biol ; 150(2-3): 91-106, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23165871

RESUMO

'What' do we call consciousness? 'When' and 'Where' in the brain do conscious states occur, and 'How' conscious processing and conscious access to a given content work? In the present paper, we present a non-exhaustive overview of each of these 4 major issues, we provide the reader with a brief description of the major difficulties related to these issues, we highlight the current theoretical points of debate, and we advocate for the explanatory power of the "global workspace" model of consciousness (Baars 1989; Dehaene and Naccache 2001; Dehaene, Changeux et al. 2006) which can accommodate for a fairly large proportion of current experimental findings, and which can be used to reinterpret apparent contradictory findings within a single theoretical framework. Most notably, we emphasize the crucial importance to distinguish genuine neural signatures of conscious access from neural events correlated with consciousness but occurring either before ('upstream') or after ('downstream').


Assuntos
Mapeamento Encefálico , Ondas Encefálicas/fisiologia , Encéfalo/fisiologia , Estado de Consciência/fisiologia , Humanos , Modelos Biológicos , Vias Neurais/fisiologia
11.
Neurology ; 77(3): 264-8, 2011 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-21593438

RESUMO

OBJECTIVE: Probing consciousness in noncommunicating patients is a major medical and neuroscientific challenge. While standardized and expert behavioral assessment of patients constitutes a mandatory step, this clinical evaluation stage is often difficult and doubtful, and calls for complementary measures which may overcome its inherent limitations. Several functional brain imaging methods are currently being developed within this perspective, including fMRI and cognitive event-related potentials (ERPs). We recently designed an original rule extraction ERP test that is positive only in subjects who are conscious of the long-term regularity of auditory stimuli. METHODS: In the present work, we report the results of this test in a population of 22 patients who met clinical criteria for vegetative state. RESULTS: We identified 2 patients showing this neural signature of consciousness. Interestingly, these 2 patients showed unequivocal clinical signs of consciousness within the 3 to 4 days following ERP recording. CONCLUSIONS: Taken together, these results strengthen the relevance of bedside neurophysiological tools to improve diagnosis of consciousness in noncommunicating patients.


Assuntos
Córtex Auditivo/fisiopatologia , Estado de Consciência/fisiologia , Estado Vegetativo Persistente/diagnóstico , Estado Vegetativo Persistente/fisiopatologia , Estimulação Acústica/métodos , Córtex Auditivo/irrigação sanguínea , Eletroencefalografia , Potenciais Evocados Auditivos/fisiologia , Feminino , Humanos , Processamento de Imagem Assistida por Computador , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Oxigênio
12.
Rev Neurol (Paris) ; 166(12): 953-8, 2010 Dec.
Artigo em Francês | MEDLINE | ID: mdl-21055783

RESUMO

Our conscious perception is not exhaustive of all the processes at work when we face a visual scene. In the light of a recent theoretical model, - the conscious global workspace model -, which states the necessary and sufficient conditions for a perceptual representation to reach conscious content, we propose here a taxonomy, which distinguishes between four types of unconscious visual processes. For each of them, we will draw close links between several neurological syndromes and experimental visual paradigms, which can be used in the laboratory with normal subjects.


Assuntos
Classificação , Doenças do Sistema Nervoso/classificação , Doenças do Sistema Nervoso/psicologia , Terminologia como Assunto , Inconsciente Psicológico , Percepção Visual/fisiologia , Humanos , Modelos Neurológicos
13.
Rev Neurol (Paris) ; 165(8-9): 702-8, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19616815

RESUMO

The relevance of multiple sclerosis for cognitive neuroscience has evolved significantly during the last decades. After a relative and enduring disinterest, the 1980's has been marked by a first wave of studies aiming at characterizing the cognitive dysfunctions associated with this disease. Once identified, and grouped under the relatively vague and nonspecific concept of "subcorticofrontal syndrome", these cognitive symptoms had to wait until the end of the 1990's to give rise to a new and vigorous resurgence of attention. Interestingly, this genuine contemporary revival of interest originates in the promotion of the very same arguments that served until there to explain the weak investment of multiple sclerosis by neuropsychology and cognitive neuroscience. The early disseminated nature of brain lesions, their dynamic and unstable nature, the prevalence of white-matter lesions, and the alteration of non-modular aspects of cognition: all these arguments have discouraged neuropsychologists for a long time. Today, these very same specific properties of multiple sclerosis offer an extremely relevant model to explore cognitive dimensions of brain plasticity, to revivify the concept of disconnection in neuropsychology, and to evaluate some neuroscientific models of consciousness.


Assuntos
Ciência Cognitiva/tendências , Esclerose Múltipla/psicologia , Neurociências/tendências , Humanos , Modelos Neurológicos , Esclerose Múltipla/fisiopatologia , Plasticidade Neuronal/fisiologia
14.
J Nutr Health Aging ; 13(6): 503-19, 2009 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19536419

RESUMO

Under the auspices of the Societe Francaise de Geriatrie et Gerontologie, a multi-disciplinary group of specialists in geriatrics, neurology, epidemiology, psychiatry, neuroradiology and nuclear medicine met with the aim of drawing up references on the methods for diagnosing and treating mild Alzheimer's disease. The critical analysis of international literature, conducted by Professor Bruno Vellas for the scientific committee, has served to support study of the latest knowledge in 2008. The multi-disciplinary group met on 14 and 15 May 2008 in order to set out the questions that this study must answer and to allocate draft studies. Thus, it has been possible to conduct a study focused on mild Alzheimer's disease, giving particular attention to diagnostic procedure, specific methods of treatment and the benefits of making a diagnosis.


Assuntos
Doença de Alzheimer/diagnóstico , Doença de Alzheimer/terapia , Programas de Rastreamento , Idoso , Doença de Alzheimer/psicologia , Biomarcadores/sangue , Biomarcadores/líquido cefalorraquidiano , Cognição , Progressão da Doença , Avaliação Geriátrica/métodos , Humanos , Programas de Rastreamento/métodos , Transtornos Mentais/diagnóstico , Índice de Gravidade de Doença
16.
Ann Fr Anesth Reanim ; 26(5): 445-51, 2007 May.
Artigo em Francês | MEDLINE | ID: mdl-17400424

RESUMO

Traumatic brain injury occurs abruptly, involves multiple specialized teams, solicits the health care system in its emergency dimension and engages the well being of the patient and his relatives for a life time period. Clinicians are faced with issues of uppermost importance: medical issues such as predicting long term neurological outcome of the comatose patient, ethical issues because of the influence of intensive care on the long term survival of patients in vegetative and minimally conscious state, legal issues as the consequence of the current law which has set a new concept of proportionality of care, social issues as the result of the very high cost of these pathologies. This review will focus on the brain explorations that are required such as CT scan, evoked potentials, electroencephalography, magnetic resonance imaging and magnetic resonance spectroscopy to provide to the clinician a multimodal assessment of the brain state to predict outcome of coma. Such assessment is mandatory to answer the crucial question of proportionality of care in these patients. However, these techniques need further validation on large series of patients before being useful on clinical practice.


Assuntos
Lesões Encefálicas/diagnóstico por imagem , Lesões Encefálicas/patologia , Assistência Terminal , Eletroencefalografia , Humanos , Imageamento por Ressonância Magnética , Espectroscopia de Ressonância Magnética , Prognóstico , Tomografia Computadorizada por Raios X , Suspensão de Tratamento
17.
Acta Neurochir (Wien) ; 147(2): 125-9; discussion 129, 2005 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-15570441

RESUMO

Lundberg (or B) waves, defined as repetitive changes in intracranial pressure (ICP) occurring at frequencies of 0.5 to 2 waves/min, have been attributed to cerebral blood flow fluctuations induced by central nervous system pace-makers or cerebral pressure autoregulation. We prospectively recorded and digitalized at a frequency rate of 10 Hz (AcqKnowledge software) the following parameters in 6 brain injured patients: mean arterial pressure, heart rate, ICP, mean flow velocity of the middle cerebral artery (MFVMCA) (transcranial Doppler WAKI) and left and right spectral edge frequency (SEFl, SEFr) of continuous electroencephalogram (EEG) recordings (Philips technologies). All patients were sedated using a combination of sufentanil and midazolam and mechanically ventilated. Cerebral electrical activity (oscillations of SEF at a mean frequency of 26+/-9 mHz) and MFVMCA fluctuations were found strongly correlated with the intracranial Lundberg B waves (mean frequency 23+/-7 mHz). These result support the existence of a neuropacemaker at the origin of the Lundberg B waves. The change in cerebral electrical activity, resulting from cerebral pacemakers, could increase cerebral metabolic rate of oxygen (CMRO2) and thus lead to an increase in cerebral blood flow and secondarily of ICP through a change in cerebral blood volume.


Assuntos
Relógios Biológicos/fisiologia , Córtex Cerebral/fisiopatologia , Circulação Cerebrovascular/fisiologia , Traumatismos Craniocerebrais/fisiopatologia , Pressão Intracraniana/fisiologia , Potenciais de Ação/fisiologia , Adolescente , Adulto , Pressão Sanguínea/fisiologia , Lesões Encefálicas/diagnóstico por imagem , Lesões Encefálicas/fisiopatologia , Córtex Cerebral/diagnóstico por imagem , Córtex Cerebral/metabolismo , Traumatismos Craniocerebrais/diagnóstico por imagem , Eletroencefalografia , Metabolismo Energético/fisiologia , Feminino , Frequência Cardíaca/fisiologia , Humanos , Masculino , Pessoa de Meia-Idade , Artéria Cerebral Média/diagnóstico por imagem , Artéria Cerebral Média/fisiologia , Monitorização Fisiológica/métodos , Monitorização Fisiológica/estatística & dados numéricos , Consumo de Oxigênio/fisiologia , Ultrassonografia Doppler Transcraniana
18.
Rev Neurol (Paris) ; 160(4 Pt 1): 395-400, 2004 Apr.
Artigo em Francês | MEDLINE | ID: mdl-15103263

RESUMO

Since the last thirty Years, scientific study of the cerebral substrate of consciousness has been marked by significant achievements, resulting in part from a rich interaction between the exploration of cognition in both brain-damaged patients and healthy subjects. Several neuropsychological syndromes contain spectacular dissociations which permit to identify principles related to the neurophysiology of consciousness. The generality of those principles can then be evaluated in the healthy subject using the combination of experimental psychology paradigms, and functional brain-imaging tools. In this paper, we review some of the recent results relevant to visual phenomenal consciousness, which is the most scientifically investigated aspect of consciousness. We isolate several of those general principles through the exploration of neuropsychological syndromes such as "blindsight", visual agnosias and neglect, and expose how their generality has been demonstrated in the healthy subject using conditions such as visual illusions or subliminal perception. Finally, we describe the bases of a scientific model of consciousness, based on the concept of a "global workspace", which takes into account the data reviewed.


Assuntos
Encéfalo/fisiologia , Estado de Consciência , Visão Ocular/fisiologia , Atenção , Humanos
19.
Psychol Sci ; 15(5): 307-13, 2004 May.
Artigo em Inglês | MEDLINE | ID: mdl-15102139

RESUMO

Fluent readers recognize visual words across changes in case and retinal location, while maintaining a high sensitivity to the arrangement of letters. To evaluate the automaticity and functional anatomy of invariant word recognition, we measured brain activity during subliminal masked priming. By preceding target words with an unrelated prime, a repeated prime, or an anagram made of the same letters, we separated letter-level and whole-word codes. By changing the case and the retinal location of primes and targets, we evaluated the invariance of those codes. Our results indicate that an invariant binding of letters into words is achieved unconsciously through a series of increasingly invariant stages in the left occipito-temporal pathway.


Assuntos
Mascaramento Perceptivo , Reconhecimento Psicológico , Percepção Visual , Vocabulário , Adulto , Feminino , Lateralidade Funcional/fisiologia , Humanos , Linguística , Imageamento por Ressonância Magnética , Masculino , Lobo Occipital/anatomia & histologia , Lobo Occipital/metabolismo , Lobo Temporal/anatomia & histologia , Lobo Temporal/metabolismo
20.
Neuroimmunomodulation ; 10(1): 1-4, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12207157

RESUMO

Anti-myelin basic protein (MBP) autoreactive T cells play a key role in the pathogenesis of multiple sclerosis. Thus, we applied the Immunoscope strategy to cerebrospinal fluid (CSF) and peripheral blood lymphocytes (PBLs) of an HLA-DR2 patient. Both compartments showed major expansion for the V(beta)13S5 chain, which was associated in peripheral blood with significant proliferation of PBLs in response to MBP and the 84-102 HLA-DR2-restricted peptide. Sequencing revealed a unique nucleotide sequence in the CSF that gives rise to the amino acid sequence V(beta)13S5-RPGQGDQETQ-J(beta)2.5 if translated. This CDR3 sequence had already been reported to be reactive against the 84-102 peptide. This specific sequence was not detected in PBLs on day 0, whereas it was readily detectable on day 6 culture samples. Thus, cell culture may lead to enrichment in a T cell clone identified as autoreactive.


Assuntos
Células Clonais/imunologia , Antígeno HLA-DR2/imunologia , Esclerose Múltipla/imunologia , Proteína Básica da Mielina/imunologia , Especificidade do Receptor de Antígeno de Linfócitos T/imunologia , Linfócitos T/imunologia , Adulto , Sequência de Aminoácidos , Autoanticorpos/sangue , Autoanticorpos/líquido cefalorraquidiano , Autoanticorpos/imunologia , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/imunologia , Células Cultivadas , Humanos , Masculino , Esclerose Múltipla/sangue , Esclerose Múltipla/líquido cefalorraquidiano , Proteína Básica da Mielina/farmacologia , Fragmentos de Peptídeos/imunologia , Receptores de Antígenos de Linfócitos T alfa-beta/efeitos dos fármacos , Receptores de Antígenos de Linfócitos T alfa-beta/imunologia , Linfócitos T/efeitos dos fármacos , Linfócitos T/metabolismo
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