Effect of Broilers Chicken Diet Supplementation with Natural and Acidified Humic Substances on Quality of Produced Breast Meat.

This study was conducted to examine the effect of two humic substances (HS) supplemented in broilers' diet on the breast meat quality of broiler chickens. In this experiment, 120 pieces of one-day-old COBB500 broiler chickens were used. Broilers were divided into three groups, each containing 40 birds with three replications (13, 13, and 14 per one pen). Fattening lasted 38 days. The first experimental diet was supplemented with 0.7% of HS (HS0.7) and the second was enriched with 0.7% of acidified HS (HSA0.7). The control group of broilers (C) was fed a basal diet without the addition of any supplements. HS0.7 samples had the highest total protein content and the lowest content of fat (p < 0.01). The effects of broiler diet and storage had a significant impact on the pH of breast samples, p < 0.001 and p < 0.05, respectively. Supplementation of HS in broiler diet positively affected the lipid oxidation of breast meat samples, as indicated by the lower malondialdehyde content (p < 0.05). HSA0.7 samples had significantly better juiciness after the storage period (p < 0.001). The quality of meat produced with supplementation of HS in broilers' feed can be considered as valuable in human nutrition due to improved protein and fat content with decreased lipid oxidation.

Concentrations of selected trace elements in organs and tissues of livestock from a polluted area.

The occurrence of cadmium (Cd), nickel (Ni) and lead (Pb) from industrial emissions were determined in the muscle and liver of cattle from agricultural farms near an industrial plant in Eastern Slovakia. In this study cows (n = 25) were slaughtered and the concentrations of Cd, Ni, Pb were analyzed with a the atomic absorption spectrophotometer. Levels reaching the highest permissible hygiene limits for toxic metals Cd, Pb and Ni were determined in 50 samples. The maximum levels of Cd, Pb and Ni were recorded in the liver (0.865; 2.324; 1.140 mg/kg, respectively) and muscle (0.300; 0.854; 0.700 mg/kg, respectively). It was concluded that the exposure to an industrial plant significantly increases the levels of contaminants in the muscle and organs of cattle, as the most susceptible livestock.

The effects of long-term cadmium exposure in turkeys: accumulation and zinc prevention.

Experiments with turkeys were conducted to determine the effects of long-term ingestion of Cd (2.0 mg Cd/day/turkey) on its accumulation in the tissues and organs. The cadmium was found especially in the muscles, livers and kidneys. The highest average content of cadmium was found in the kidneys (1.09 mg/kg). The contents of Cd in the kidneys and livers were 19 times and 14 times (respectively) higher compared to the muscles. The administration of Zn (72 mg / day / turkey) along with high doses of Cd, significantly (P < 0.001) reduced the concentrations of Cd in the organs of the experimental animals. The average concentrations of cadmium in the kidneys and livers of turkeys from the CdZn group were 43 % and 48 % (respectively) lower than the average concentrations in the same organs in the turkeys from the group which received only Cd.

Content of selected metals in muscle of cyprinid fish species from the Nitra River, Slovakia.

OBJECTIVES: This study presents a rate of bioaccumulation of some metal concentrations in the muscle of five common cyprinid fish species (gudgeon - Gobio gobio, Europen chub - Leuciscus cephalus, barbel - Barbus barbus, roach - Rutilus rutilus, and nase - Chondrostoma nasus). DESIGN: Besides, correlations among selected metals as well as standard length and total weight and order of metal accumulation in the fish muscle were determined. RESULTS: Recorded metal concentrations (mg/kg wet weight basis) ranged as follows: Fe 4.23-22.95, Mn 0.10-0.72, Zn 16.26-166.90, Cu 15.39-25.97, Ni 0.00-3.72, Pb 0.00-1.81, and Cd 0.00-0.11. CONCLUSIONS: The Slovak permissible limit for Cu (10.0 mg/kg), Ni (0.5 mg/kg), Pb (0.2 mg/kg) and Cd (0.05 mg/kg) defined in the Codex Alimentarius for safe human consumption exceeded in 100%, 12%, 6% and 10% of analyzed samples for Cu, Ni, Pb and Cd respectively. On an average, the order of metal concentrations in the fish muscle was: Zn>Cu>Fe>Mn>Ni>Pb>Cd.

Effect of oral cadmium and zinc application on leukocytes biological activity in Japanese quails (Coturnix coturnix japonica).

Objective of this study was to investigate changes in biological activity of blood leukocytes in Japanese quails, caused by zinc and cadmium administration. Four groups of Japanese quails were used. Three experimental groups of quails were exposed either to Cd (0.12 mg Cd/quail), Zn (4 mg Zn/quail) or a combination of Cd and Zn (0.12 mg Cd, 4 mg Zn/quail), which were added daily to the drinking water. The fourth group was the control group. The metabolic activity of phagocytes and mitogenic activation of lymphocytes to phytohemaglutinine (PHA) were determined on day 37, 58 and 118 of exposure. The numbers of peripheral blood leukocytes of Japanese quail after cadmium and zinc addition in all groups of birds were without significant differences, however, the functional activities of phagocytes and lymphocytes in the Cd-group of quails were significantly decreased. The metabolic activity of phagocytes decreased significantly at all time points analysed (P < 0.05-0.001) in comparison to the control group and the Cd-Zn group. Similarly, the response of lymphocytes to PHA activation in the Cd exposed group of quails decreased significantly on day 58 and 118 of exposure when compared to the control group (P < 0.05) and Zn-group of quails (P < 0.01). Zn in combination with Cd eliminated the immunotoxic effect of Cd on metabolic activity of phagocytes and improved lymphocyte answer to PHA, when compared to the Cd-group on day 58 and 118 of metal administration. The results of the present study indicate that cadmium caused a significant decrease of metabolic activity of phagocytes and mitogenic activation of lymphocytes in peripheral blood of Japanese quails. Simultaneous administration of Zn and Cd eliminated the immunotoxic effect of Cd on functional activity of phagocytes and lymphocytes, and zinc improved both investigated functional parameters of immune cells.

The effect of the probiotic strain Enterococcus faecium DSM 7134 in combination with natural humic substances on performance and health of broiler chickens.

The present study investigated the effect of the probiotic strain Enterococcus faecium DSM 7134 (2 x 10(7) cfu per g of feed) (probiotic group), natural humic substances (7g x kg(-1) feed) (HS group), and the combination of both (probiotic + HS group) on the performance, serum biochemistry, and biological activity of leucocytes of broiler chickens. Average body weight of chickens in different phases as well as throughout the observation period under different treatments did not differ significantly. Final body weight and feed conversion ratio (FCR) reached in 41 days of trial were 2542.5; 2568.9; 2610.5, 2516.6 g and 1.93; 1.81; 1.78; 1.88 kg for HS, probiotic, probiotic + HS and control group respectively. The best performance variables including the European efficiency index (EEI) were observed in the probiotic + HS group, where the effect of the probiotic supplementation was improved by HS. There were no significant differences among groups in the serum levels of total protein, glucose, cholesterol, aminotransferase and phosphorus. A significant decrease of total lipids in the probiotic group (P < 0.01) and probiotic + HS group (P < 0.001) groups, triglycerides levels in the HS (P < 0.01), probiotic and probiotic + HS (P < 0.001) groups on day 35, calcium level of the probiotic (P < 0.05) and probiotic + HS (P < 0.01) groups on day 41 and alkaline phosphatase levels in the probiotic group (P < 0.01) on day 14 were observed in comparison to the control group. Significantly higher values of indices of metabolic activity of phagocytes (IMA) after 14, 35 and 41 days of the experiment were observed in the probiotic + HS group (P < 0.01, P < 0.05) when compared with the control group. No adverse health effects were observed in experimental groups The results of the present study indicate that HS and E. faecium DSM 7134 had no significant effect on performance. However an improvement in FCR and EEI was observed which is believed to be due to the significant increase of indices of metabolic activity of phagocytes, caused by HS when combined with. E. faecium DSM 7134.

The effect of cadmium in combination with zinc and selenium on ovarian structure in Japanese quails.

In this study the effect of cadmium, cadmium+selenium and cadmium+zinc administration on the ovarian structure in Japanese quails was studied. The morphometric analysis of the relative volume of primary follicles detected the highest value in control group with a similar value in the group with administration of cadmium with selenium. Lower relative volume is reported in group with cadmium and zinc administration and the group with simple cadmium administration (P < 0.05). The relative volume of growing follicles was very similar in all studied groups (11.33-15.35%), and the relative volume of stroma was very stable (82.59-86.45%). In the evaluation of the number of follicles undergoing atresia detected significantly higher number of atretic primary follicles as well as atretic growing follicles in the group with cadmium administration and cadmium with selenium administration in comparison with control group. In comparison of normal and atretic follicles we report the most negative effect of single cadmium administration on ovarian structure. Selenium co-administration shows protective effects but only the co-administration with zinc prevent significant cadmium ovarian alterations.

Testing two Lactobacillus plantarum and Lactobacillus acidophilus strains for their suitability as a lipoid probiotic.

Two strains of lactobacilli (Lactobacillus acidophilus T-135 and Lactobacillus plantarum 4/97) were selected in order to study their inhibitory properties against frequent udder pathogens (Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus uberis, Salmonella enteritidis and Bacillus pumilus), their production of organic acids as well as their ability to survive on the teat skin, the teat duct mucosa and in a lipoid emulsion. Both strains inhibited the tested pathogenic microbes and survived on the investigated surfaces and in an emulsion for more than 6 hours and 11 days, respectively.