RESUMO
Most of pharmaceutical agents display several or even many biological activities. It is obvious that testing even one compound for thousands of biological activities is a practically not reasonable task. Therefore, computer-aided prediction is the method of choice for the selection of the most promising bioassays for particular compounds. Using PASS Online software, we determined the probable antimicrobial activity of the 31 steroid derivatives. Experimental testing of the antimicrobial activity of the tested compounds by microdilution method confirmed the computational predictions. Furthermore, P. aeruginosa and C. albicans biofilm formation was investigated. Compound 11 showed a biofilm reduction by 42.26% at the MIC of the tested compound. The percentages are lower than ketoconazole, but very close to its activity.
Assuntos
Anti-Infecciosos , Antifúngicos , Simulação de Acoplamento Molecular , Antifúngicos/farmacologia , Relação Estrutura-Atividade , Antibacterianos/farmacologia , Hidrazonas/farmacologia , Relação Quantitativa Estrutura-Atividade , Anti-Infecciosos/farmacologia , Candida albicans , Esteroides/farmacologia , Testes de Sensibilidade Microbiana , Estrutura MolecularRESUMO
Several new N-containing epiandrosterone derivatives modified by phenylacetic acid chloride were synthesized for biological activity studies. Compounds with antiviral activity were discovered among them and 3ß-hydroxy-1'-aryl-3'-methyl-5'-androstano[17,16-d]pyrazolines prepared by us earlier.
RESUMO
A study of the antiviral activity of several new hydrazones and amines and amides of 5α-steroids that were synthesized by us earlier found highly and moderately active compounds. The structures of the synthesized compounds were proven using IR, PMR, 13C NMR, and mass spectral data.