RESUMO
This study was designed to compare the effects on goat spermatozoa cryosurvival of nano-lecithin-based (NL), lecithin-based (L) and egg yolk-based (EY) extenders. Ejaculates were collected from four fertile goats using artificial vagina and diluted with nine extenders. NL and L were tested at concentrations 1%, 2%, 3% and 4% (w/v), versus 15% (v/v) egg yolk-based extender. Overall, sperm quality (higher motility, viability and HOST, and lower apoptosis) was higher for NL than for L treatments (Pâ¯<â¯0.05 for most cases, except for 1%). NL at 1% and especially at 4% showed lower motility and viability than 2% or 3% NL. NL at 2% achieved a better performance (Pâ¯<â¯0.05) than EY for VCL (131.5⯱â¯1.3 vs. 120.3⯱â¯1.9⯵m/s), VSL (43.9⯱â¯1.5 vs. 35.8⯱â¯1.4⯵m/s), LIN (35.7⯱â¯0.6 vs. 29.3⯱â¯0.8%), WOB (47.0⯱â¯0.5 vs. 43.9⯱â¯0.9%) and viability (66.4⯱â¯1.7 vs. 52.7⯱â¯1.9%). Late apoptotic spermatozoa were also lower in 2% NL compared to EY (16.0⯱â¯0.5 vs. 26.3⯱â¯1.1%, Pâ¯<â¯0.001). EY and 2% NL were compared in an IVF trial, with no significant differences in cleavage (68.8 vs. 70.8%) or blastocyst ratios (21.3 vs. 20.8%). In conclusion, using 2% nanolecithin in semen dilution could improve sperm cryosurvival of goat.