RESUMO
BACKGROUND: We investigated the effect of bioactive glass and zinc oxide nanoparticles on enamel remineralization, as well as their antimicrobial effect on cariogenic microbes. This is the first study that investigated the properties of bioactive glass and zinc oxide nanoparticles with mixed materials. METHODS: Fluoride gel (F), bioactive glass microparticles (µB), bioactive glass nanoparticles (nB), zinc oxide nanoparticles (Z), and a mixed suspension of nB and Z (nBZ) were prepared and characterized by scanning and transmission electron microscopy, zeta potential measurement, X-ray diffraction, and acid buffering capacity testing. Further, we performed a remineralization cycle test of 28 days, and nanoindentation testing was carried out during the immersion period, and then the enamel surfaces were examined using scanning electron microscopy. Additionally, the antimicrobial effects of the sample suspensions were evaluated by measuring their minimum microbicidal concentrations against various cariogenic microbes. RESULTS: Our results revealed that nB had a near-circular shape with an amorphous structure and a considerably large specific surface area due to nanoparticulation. Additionally, nB possessed a rapid acid buffering capacity that was comparable to that of µB. In the remineralization test, faster recovery of mechanical properties was observed on the enamel surface immersed in samples containing bioactive glass nanoparticles (nB and nBZ). After remineralization, demineralized enamel immersed in any of the samples showed a rough and porous surface structure covered with mineralized structures. Furthermore, nBZ exhibited a broad antimicrobial spectrum. CONCLUSIONS: These results demonstrated that bioactive glass and zinc oxide nanoparticles have superior demineralization-suppressing and remineralization-promoting effects.
RESUMO
Surface pre-reacted glass-ionomer (S-PRG) technology allows for the release of multiple ions. This study was performed to investigate the remineralization of etched enamel after removal of the bracket using a novel paste containing S-PRG filler. Surfaces of polished enamel were etched with phosphoric acid and then subdivided into two regions. Bracket bonding resin was thinly applied to one region. After 24-hr immersion in artificial saliva, the bonding resin on the enamel surfaces was removed by grinding with tungsten carbide bur at low-speed, followed by polishing with one of four different polishing systems: (a) nonfluoridated paste (NF); (b) fluoridated paste (F); (c) S-PRG filler-containing paste (S-PRG); or (d) nonfluoridated plus S-PRG filler-containing paste (NF + S-PRG) (n = 15). The polished specimens were then immersed in an artificial saliva solution for 7 days. Nanoindentation testing of enamel surfaces was performed and their mechanical properties were compared. Representative specimens were examined with scanning electron microscope. In all specimens, the mechanical properties of the enamel surfaces were markedly degraded by acid etching. However, the mechanical properties of both regions (etched and resin-infiltrated enamels) showed recovery after polishing and 7-day immersion. Polishing with NF + S-PRG paste led to significant recovery of mechanical properties compared to polishing with NF or S-PRG paste alone, and remineralization was equivalent to that seen with F paste. Porous etched enamel surfaces were filled with a remineralization layer after each polishing procedure and 7-day immersion in all polishing groups. Polishing using NF + S-PRG paste can facilitate enamel remineralization after bracket removal.
