Predictability of the progress of secondary osteoarthritis after developmental dislocation of the hip, utilizing inferior edge (of the teardrop)--center (of the femoral head) distance.

We followed-up a group of patients in their youth and then in middle age after they had been treated for developmental dislocation of the hip, and studied whether we could predict the progress of osteoarthritis of the hip when the patients were in the "youth" stage. We studied 21 hips of 21 patients with unilateral dislocation that could be examined twice, in 1975 and 1995, in patients who were treated at our hospital between 1953 and 1963. We measured the acetabular-head index (AHI), center-edge angle (CE angle), and the Sharp angle, and our created index (inferior edge of the teardrop - center of the femoral head distance), and we divided this index by the distance of the inferior edges of the teardrops on both sides. Using these measured values, we studied whether we could predict changes in clinical and radiographic evaluation after a follow-up of 20 years. There was no significant correlation of clinical and radiographic results and AHI, CE angle, and the Sharp angle; however, there was a significant correlation with our created index. The index we created is relatively easily measured and enables us to make a more precise prognosis, in comparison with previously developed indices.

Odor discrimination of 'cAMP-' and 'IP3-increasing' odorants at high temperature and at high NaCl concentration in turtle olfactory system.

The ability of the turtle olfactory system to discriminate between various cAMP- and IP3-increasing odorants at high temperature and at high NaCl concentration in the olfactory bulb was examined by the cross-adaptation technique. The degrees of discrimination in high [Na+] solution were similar to those in normal Ringer's solution, suggesting that selectivities of receptors coupled with cAMP- and IP3-dependent pathways are similar to those coupled with both cAMP- and IP3-independent pathways. The mean values of the degree of discrimination among the IP3-increasing odorants were higher than those among the cAMP-increasing odorants at high temperature and at high [Na+] concentration. The degrees of discrimination among the IP3-increasing odorants at 40 degrees C were greater than those at 25 degrees C, while those among the cAMP-increasing odorants at 40 degrees C were similar to those at 25 degrees C, suggesting that the features of the receptors of cAMP-increasing odorants are different from those which respond to IP3-increasing odorants.

Ca2+ and Cl(-)-dependence of the turtle olfactory response to odorants and forskolin.

In the turtle olfactory system, large responses to odorants appeared after application of cAMP of forskolin at high concentrations to the isolated olfactory receptor neurons or the olfactory epithelium, suggesting that a cAMP-independent pathway greatly contributes to the generation of odor responses. We measured the effects of the mucosal Ca2+ and Cl- concentrations and a Ca(2+)-activated Cl(-)-channel blocker, 4-acetamide-4'-(isothiocyano) stilbene-2,2'-disulphonic acid (SITS), upon olfactory bulbar responses to explore the contribution of the Ca(2+)-activated Cl(-)-channels to cAMP-dependent and independent pathways. Elimination of mucosal Ca2+ by addition of 2 mM EGTA to the stimulating solution partially inhibited the cAMP-independent responses to 0.1 mM citralva but did not affect those to 0.1 mM lilial or 0.1 mM l-carvone. Substitution of mucosal Cl- with gluconate slightly enhanced the total response to 0.1 mM citralva but practically did not affect the responses to other odorants tested. SITS (2 mM) partially inhibited the responses to 50 microM forskolin and 0.1 mM citralva but did not affect the cAMP-independent response to 0.1 mM citralva in Ca(2+)-free Ringer's solution. These results suggested that the Ca(2+)-activated Cl- channels do not contribute to the generation of the cAMP-independent responses, but that they partially contribute to the generation of the cAMP-dependent responses in the turtle.

Odor discrimination of "IP3-" and "cAMP-increasing" odorants in the turtle olfactory bulb.

The ability of the turtle olfactory system to discriminate between various odorants that increase levels of adenosine 3',5'-cyclic monophosphate (cAMP) and inositol trisphosphate (IP3) in the olfactory bulb was examined by the cross-adaptation technique and analyzed by multidimensional scaling. The mean values of the degree of discrimination among the IP3-increasing odorants were higher than those among the cAMP-increasing odorants, and were similar to those between cAMP- and IP3-increasing odorants, suggesting that the features of the receptors of cAMP-increasing odorants are different from those which respond to IP3-increasing odorants. Analysis by multidimensional scaling suggested that differences in second messenger pathways are not related to detecting odor quality in the turtle olfactory system.

Analysis of odor discrimination by multidimensional scaling at different temperatures.

Discrimination of odorants by the turtle olfactory bulb at 25 degrees and 37 degrees C was examined by the cross-adaptation technique and analysed by multidimensional scaling. Analysis by multidimensional scaling suggests that at 25 degrees C odorants are grouped according to their odor qualities in the turtle olfactory system. At 37 degrees C, the cluster formation of odorants, which have a similar odor quality,such as minty and floral alcohol odorants and molecular structure, in the plot of multidimensional scaling was poor, indicating that the ability of odor grouping according to their odor qualities was low at 37 degrees C.

Reconfirmation of indirect induction of radiogenic lymphomas using thymectomized, irradiated B10 mice grafted with neonatal thymuses from Thy 1 congenic donors.

An experiment was conducted to reexamine earlier observations that lymphomas could develop from lymphocytes present in nonirradiated thymuses grafted into thymectomized, fractionally (170 R, 4 doses) irradiated mice by using B10. Thy 1 congenic donor-host combinations. The results indicated that: (a) 37 of 91 thymectomized, fractionally irradiated B10. Thy 1.2 mice which were grafted s.c. with 7-day-old thymuses from B10. Thy 1.1 donor mice had developed frank lymphomas between 90 and 270 days after thymus grafting; (b) 28 of 37 lymphomas developed in this group were typed individually with respect to Thy 1 alloantigens by the cytotoxicity assay using monoclonal anti-Thy 1.1 (T-11-D7) and anti-Thy 1.2 (F7D5) antibodies plus complement. It was shown that 21 thymic lymphomas (75%) had originated from lymphocytes of the nonirradiated thymus grafts and 5 tumors (17.9%) from cells of the irradiated hosts; 2 thymic lymphomas (7.1%) manifested no Thy 1 antigens; (c) lymphoma cells originated from both nonirradiated thymus grafts and irradiated hosts possessed chromosome abnormalities, which were mainly numerical changes of some chromosomes or polyploidizations.