Nanobodies as powerful pulmonary targeted biotherapeutics against SARS-CoV-2, pharmaceutical point of view.

Background Since December 2019, the newly emerged SARS-CoV-2 virus continues to infect humans and many people died from severe Covid-19 during the last 2 years worldwide. Different approaches are being used for treatment of this infection and its consequences, but limited results have been achieved and new therapeutics are still needed. One of the most interesting biotherapeutics in this era are Nanobodies which have shown very promising results in recent researches. Scope of review Here, we have reviewed the potentials of Nanobodies in Covid-19 treatment. We have also discussed the properties of these biotherapeutics that make them very suitable for pulmonary drug delivery, which seems to be very important route of administration in this disease. Major conclusion Nanobodies with their special biological and biophysical characteristics and their resistance against harsh manufacturing condition, can be considered as promising, targeted biotherapeutics which can be administered by pulmonary delivery pharmaceutical systems against Covid-19. General significance Covid-19 has become a global problem during the last two years and with emerging mutant strains, prophylactic and therapeutic approaches are still highly needed. Nanobodies with their specific properties can be considered as valuable and promising candidates in Covid-19 therapy.

Health risk assessment and source apportionment of polycyclic aromatic hydrocarbons associated with PM10 and road deposited dust in Ahvaz metropolis of Iran.

The objective of this study was to compare the characteristics of polycyclic aromatic hydrocarbons (PAHs) in PM10 and road dust samples, as well as to identify and quantify the contributions of each source profile using the positive matrix factorization (PMF) receptor model. Health risk assessment was carried out using toxic equivalency factors and incremental lifetime cancer risk (ILCR), which quantitatively estimate the exposure risk for age-specific groups. PM10 samples were collected on PTFE filters in the metropolitan area of Ahvaz. Road dust samples were also collected from all over the urban areas with different land uses. Total PAH concentrations in PM10 and road dust samples were 0.5-25.5 ng/m3 and 49.3-16,645 µg/kg, respectively. Pyrene was the highest PAH in the PM10 profile, whereas fluoranthene became the highest PAH in the road dust. Abundance of benzo[ghi]perylene at PM10 and road dust samples suggested a source indicator for traffic emissions. The results demonstrate that in 36.5% of samples, PM10 concentrations exceed the maximum concentration level recommended by EPA. A multiple linear regression model was used to estimate the influence of meteorological parameters (temperature, wind speed, and relative humidity) on buildup of PAHs. All of PAH species show higher concentrations during the cold and typical days rather than the dust event days and warm periods. PMF analysis showed that vehicular emissions (50.6%) and industrial activities (especially steel industries) (30.4%) were first two sources of PAHs bounded with PM10, followed by diesel emissions (11.6%) and air-soil exchange (7.4%). For road dust samples, three common sources were also identified: vehicular traffic (48%), industrial activities (42.3%), and petrogenic sources (9.7%), in line with that of diagnostic molecular ratios results. According to the results of health risk assessment model, the ILCR of exposure to PAHs associated with PM10 and road-deposited dust was higher than the guidelines of USEPA, indicating high carcinogenic risk.

Risk-based assessment of soil pollution by potentially toxic elements in the industrialized urban and peri-urban areas of Ahvaz metropolis, southwest of Iran.

The main purpose of this research was to assess the level of contamination, source identification, geochemical fractionation, and health risk of potentially toxic elements (PTEs) in industrial soils from urban and peri-urban areas of Ahvaz city. A total of 92 surface soil samples were gathered and concentrations of sixteen PTEs were measured using aqua regia digestion by an inductively coupled plasma mass spectrometry (ICP-MS). Possible sources of PTEs were quantitatively determined by positive matrix factorization (PMF) receptor model combined with geostatistical analyses and geochemical methods. The results showed that long-term industrial activities have enhanced the levels of some PTEs particularly Pb, Hg, Zn, Mo, Sb, Fe, Cu, and Cd to different extents. Contamination indices including geoaccumulation index, pollution index, and Nemerov integrated pollution index along with multivariate statistical analyses confirmed that steel and iron industries are the most contaminating industries in the study area. The outcomes attained from Kruskal-Wallis test affirmed that there was a significant difference among the concentrations of As, Hg, Mn, Cu, Fe, Pb, Mo, Cd, V, Zn, and Sb in soils around different industrial clusters. Among the studied elements, the highest mobility factors belonged to Zn (81.49%), Pb (76.71%), Cu (71.65%), Hg (66.23%), Mn (62.48%), and Mo (59.27%), respectively. Also, the PMF model showed that steel and iron industries (51.2%) and natural sources (23.4%) are the main sources of PTEs, followed by industrial towns (16.7%) and power plants (8.7%). This is in line with the results of principal component analysis (PCA). Majority of the measured PTEs showed the highest bioavailability in surface soils collected from around the steel and iron industries. Also, based on the outcomes of the health risk assessment model, particular attention should be paid to Hg, Pb, Zn, and Cu in industrial soils of Ahvaz.

Contamination Level, Source Identification and Risk Assessment of Potentially Toxic Elements (PTEs) and Polycyclic Aromatic Hydrocarbons (PAHs) in Street Dust of an Important Commercial Center in Iran.

The geochemical nature and health hazards of potentially toxic elements (PTEs) and polycyclic aromatic hydrocarbons (PAHs) in the street dust of Bandar Abbas, Iran, are investigated in this study based on 27 street dust samples. Mean concentrations of Cu, Pb, Zn, As, Sb, and Hg revealed elevated concentrations as compared to the world soil average. Calculated enrichment factors (EFs) indicated that there is very high contamination in dust particles owing to anthropogenic emissions. Two main sources of PTEs are traffic emissions (Cu, Pb, Zn, Co, Mn, Fe, As, Cd, Sb, and Hg) and resuspended soil particles (Al, Ti, Ni, and Cr). Statistical analysis shows that Al, Mn, Ni, Ti, Cr, Fe, and Co are geogenic, whereas PAHs are mainly derived from traffic emissions. Values of incremental lifetime cancer risk (ILCR), as derived from a modified model of the United State Environmental Protection Agency (USEPA), indicate that Bandar Abbas residents are potentially exposed to high cancer risk, especially via dust ingestion and dermal contact, whereas the level of hazard index (HI), hazard quotients (HQ), and cancer risk associated with exposure to the elements in street dust fall lower than threshold values representative of health risks.

Source apportionment and health risk assessment of potentially toxic elements in road dust from urban industrial areas of Ahvaz megacity, Iran.

This study investigates the occurrence and spatial distribution of potentially toxic elements (PTEs) (Hg, Cd, Cu, Mo, Pb, Zn, Ni, Co, Cr, Al, Fe, Mn, V and Sb) in 67 road dust samples collected from urban industrial areas in Ahvaz megacity, southwest of Iran. Geochemical methods, multivariate statistics, geostatistics and health risk assessment model were adopted to study the spatial pollution pattern and to identify the priority pollutants, regions of concern and sources of the studied PTEs. Also, receptor positive matrix factorization model was employed to assess pollution sources. Compared to the local background, the median enrichment factor values revealed the following order: Sb > Pb > Hg > Zn > Cu > V > Fe > Mo > Cd > Mn > Cr ≈ Co ≈ Al ≈ Ni. Statistical results show that a significant difference exists between concentrations of Mo, Cu, Pb, Zn, Fe, Sb, V and Hg in different regions (univariate analysis, Kruskal-Wallis test p < 0.05), indicating the existence of highly contaminated spots. Integrated source identification coupled with positive matrix factorization model revealed that traffic-related emissions (43.5%) and steel industries (26.4%) were first two sources of PTEs in road dust, followed by natural sources (22.6%) and pipe and oil processing companies (7.5%). The arithmetic mean of pollution load index (PLI) values for high traffic sector (1.92) is greater than industrial (1.80) and residential areas (1.25). Also, the results show that ecological risk values for Hg and Pb in 41.8 and 9% of total dust samples are higher than 80, indicating their considerable or higher potential ecological risk. The health risk assessment model showed that ingestion of dust particles contributed more than 83% of the overall non-carcinogenic risk. For both residential and industrial scenarios, Hg and Pb had the highest risk values, whereas Mo has the lowest value.

The role of selenium and selected trace elements in the etiology of esophageal cancer in high risk Golestan province of Iran.

Selenium (Se) deficiency is reported by some authors to be an important factor in the etiology of esophageal cancer (EC) in the Golestan province of Iran. In order to further investigate the role of Se and selected trace elements in the occurrence of EC disease, 663 samples including 206 cultivated soils, 247 sediments, 45 loess deposits and 165 grain samples from 45 villages in the Golestan province of Iran were collected and analyzed. Villages in the study area were classified into 2 groups according to the EC incidence in the local population. The results of this study demonstrate that, contrary to the expected trend, total Se concentrations in soil, grain, sediment, and loess samples increase from the low to the high EC areas suggesting that Se deficiency does not play a major role in the etiology of EC. On the other hand, antimony (Sb), and strontium (Sr) content in soil, grain, loess, and sediment samples is much higher in the high esophageal cancer area, which may be a significant factor. Total zinc (Zn) concentrations in soil, grain, loess, and sediment samples decrease from the low to the high cancer areas. Therefore, Zn deficiency may be a significant factor in EC incidence rate in the Golestan province.

Quality of drinking water and high incidence rate of esophageal cancer in Golestan province of Iran: a probable link.

Golestan province in north Iran is known to be a high-risk area for esophageal cancer (EC). Of a long list of multiple risk factors, this study focuses on a possible link between the epidemiologic patterns of EC and the anomalous concentration of some ions and elements in the drinking water sources. A total of 183 samples from 45 villages covering a wide range of EC mortality rates are collected and analyzed. The results demonstrate that NO(3)(-), SO(4)(2-), Sb, and Sr exceed the recommended maximum concentration level (MCL) in drinking water. This is more prominent in the villages with high esophageal cancer mortality rate, suggesting a possible link between EC incidence and water quality. Se concentration in drinking water increases from low to the high EC areas, a finding contrary to the expected trend. It is concluded that Se deficiency does not play a major role in the etiology of EC in the Golestan province. The statistical results obtained from Mann-Whitney and Kruskal-Wallis tests along with cluster analysis are consistent with the observed trend of EC mortality rate in Golestan province.