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1.
J Oleo Sci ; 73(4): 619-623, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38556295

RESUMO

The distribution of electrolytes near the air/water surface plays an essential role in many processes. While the general distribution is governed by classic Poisson-Boltzmann statistics, the analytical solution is only available for symmetric electrolytes. From the recent studies in the literature, it is evident that surface adsorption is dependent on specific ions as well as the H-bond structure at the surface. Experimental data can capture the macro properties of the surface, such as surface tension and surface potential. Yet, the underpinning mechanisms behind this experimental macro-observation remain unclear. To address the challenge, we developed a framework combining experimental studies and numerical calculations. The model was developed for electrolytes with unequal cationic and anionic charges. The asymmetric model was successfully applied to describe the surface charge of MgCl 2 aqueous solution. The results can be explained by the role of cationic size and charge on the surface layer.


Assuntos
Eletrólitos , Água , Água/química , Eletrólitos/química , Íons , Tensão Superficial , Adsorção
2.
Langmuir ; 40(12): 6484-6492, 2024 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-38470245

RESUMO

Interactions between the sigma1 receptor agonist PRE-084 and various lipid monolayers, including dipalmitoylphosphatidylcholine (DPPC), DPP-ethanolamine (DPPE), DPP-glycerol (DPPG), DPP-serine (DPPS), palmitoylsphingomyelin (PSM), and cholesterol (Ch), were investigated to elucidate the effects of PRE-084 on membrane fluidity and stability. Their interactions with sigma1 receptor agonists have potential implications for neuroprotection, antidepressant, analgesic, and cognitive enhancement effects. In this study, we observed that the presence of PRE-084 in the subphase led to increased fluidity in DPPC and DPPE monolayers, whereas decreasing fluidity was observed in DPPG, DPPS, and PSM monolayers. The interaction of PRE-084 with Ch monolayers was found to be distinct from its interaction with other lipids. Fluorescence microscopy images revealed changes in the size and shape of liquid-condensed domains in the presence of PRE-084, supporting the notion of altered membrane fluidity. Our findings provide new insights into the interaction of PRE-084 with lipid monolayers and its potential implications for biological and membrane science.


Assuntos
1,2-Dipalmitoilfosfatidilcolina , Fluidez de Membrana , Éteres Fenílicos , Microscopia de Fluorescência
3.
Colloids Surf B Biointerfaces ; 211: 112333, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35038654

RESUMO

The surface chemistry of the inverse electron-demand Diels-Alder (IEDDA) reaction at the air-water interface is elucidated. Tetrazine (C18-Tz) and norbornene derivatives (C16-NCA) were used as the reactants. Langmuir monolayers of C18-Tz, C16-NCA, and their binary mixtures were prepared on aqueous substrates. The surface properties were analyzed using the surface pressure (π)-molecular area (A) and surface potential (ΔV)-A isotherms, as well as fluorescence microscopy to monitor the progress of the reaction. First, to provide comparison data to evaluate the reaction on the surface, the two components were mixed in stock solutions of organic solvents for the IEDDA reaction. The Langmuir monolayer spread from the reaction solution was characterized as a function of the reaction time. In the subsequent experiments, the Langmuir monolayers were deposited onto the surface of the substrate solutions by spreading from separate stock solutions of C18-Tz and C16-NCA. The variation of the surface behavior of the monolayers with the molecular area, surface composition of the two components, compression speed of the monolayers, and the temperature was studied. We discuss the effects of the air phase in the reaction field on the reaction efficiency by comparing the results obtained from the two methods.


Assuntos
Elétrons , Compostos Heterocíclicos , Reação de Cicloadição , Norbornanos/química , Água
4.
Biophys Rev (Melville) ; 3(2): 021303, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38505415

RESUMO

Tetrazine (Tz) is an emerging bioorthogonal ligand that is expected to have applications (e.g., bioimaging) in chemistry and chemical biology. In this review, we highlight the interactions of reduced tetrazine (rTz) derivatives insoluble in aqueous media with biological membrane constituents or their related lipids, such as dipalmitoyl-phosphatidylcholine, dipalmitoyl-phosphatidylethanolamine, dipalmitoyl-phosphatidylglycerol, palmitoyl-sphingomyelin, and cholesterol in the Langmuir monolayer state at the air-water interface. The two-component interaction was thermodynamically elucidated by measuring the surface pressure (π) and molecular area (A) isotherms. The monolayer miscibility between the two components was analyzed using the excess Gibbs energy of mixing and two-dimensional phase diagram. The phase behavior of the binary monolayers was studied using the Brewster angle, fluorescence, and atomic force microscopy. This study discusses the affinities of the rTz moieties for the hydrophilic groups of the lipids used.

5.
Langmuir ; 36(36): 10750-10755, 2020 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-32830502

RESUMO

Bio-orthogonal inverse electron demand Diels-Alder (IEDDA) reactions between liposomes containing a tetrazine-based (Tz) compound and 2-norbornene (2-NB) could be a novel trigger for accelerating drug release from the liposomes via temporary membrane destabilization, as shown in our previous report. Herein, we evaluated the in vitro drug release using NB derivatives with carboxyl groups [5-norbornene-2-carboxylic acid (NBCOOH) and 5-norbornene-2,3-dicarboxylic acid (NB(COOH)2)] to investigate the effects of substituents at the NB backbone on the drug release rate. First, POTz-liposome composed of a Tz compound (2-hexadecyl-N-(6-(6-(pyridin-2-yl)-1,2,4,5-tetrazin-3-yl)pyridin-3-yl)octadecanamide) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) were prepared. The mass spectrometry analysis revealed the binding of NB derivatives to the Tz compound via the IEDDA reaction after the POTz-liposome reacted with the NB derivatives. Indium-111-labeled diethylenetriaminepentaacetic acid (111In-DTPA) was encapsulated inside the liposomes, and the drug release rate was quantified by measuring radioactivity. At 24 h after incubation with 2-NB, NBCOOH, and NB(COOH)2, the release rates of 111In-DTPA from POTz-liposome were 21.0, 80.8, and 23.3%, respectively, which were significantly higher than those of POTz-liposome that was not treated with NB derivatives (4.2%), indicating the involvement of the IEDDA reaction for prompting drug release. Additionally, a thermodynamic evaluation using Langmuir monolayers was conducted to explore the mechanism of the accelerated drug release. An increase in membrane fluidity and a reduction in intermolecular repulsion between POPC and the Tz compound were observed after the reaction with NB derivatives, especially for NBCOOH. Thus, the IEDDA reaction in the liposomal membrane could be a potent trigger for accelerating the release of encapsulated drugs by regulating membrane fluidity and intermolecular repulsion in the liposomal membrane.


Assuntos
Elétrons , Lipossomos , Reação de Cicloadição , Fluidez de Membrana , Membranas
6.
Chemphyschem ; 21(17): 1966-1970, 2020 09 02.
Artigo em Inglês | MEDLINE | ID: mdl-32710449

RESUMO

We found that monolayers of dipalmitoylphosphatidylcholine (DPPC) and semi-fluorinated tetrablock di(F10H16) self-assemble to form a new type of large, complex flower-like patterns on the surface of water and on solid substrates. The hierarchical organization of these unusual self-assemblies was investigated using compression and surface potential isotherms, in situ fluorescence and Brewster angle microscopies, and atomic force microscopy after transfer.

8.
J Oleo Sci ; 69(6): 557-562, 2020 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-32404550

RESUMO

The solubilization of poorly water-soluble progesterone derivatives into micelles of a gemini surfactant was investigated in an aqueous medium. The aqueous solubility at different temperatures was determined spectroscopically using an ultraviolet visible light spectrophotometer. Thermodynamic parameters for the solubilization were calculated under the basis of the solubility change against temperature. The solubility of progesterone was quite low and remained constant below the critical micelle concentration (cmc) of the surfactant. On the other hand, the solubility increased considerably with increasing surfactant concentration above the cmc. It was suggested that the solubilizates were located in the vicinity of polar regions of micelles.


Assuntos
Micelas , Progesterona/química , Tensoativos , Solubilidade , Soluções , Espectrofotometria Ultravioleta , Temperatura , Termodinâmica , Água
9.
J Oleo Sci ; 69(6): 519-528, 2020 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-32404551

RESUMO

The surface charge/surface potential of the air/water interface plays a key role in many natural and industrial processes. Since the first decade of the 20th century, there are many theoretical proposals to describe the surface charge in the presence of different moieties. However, a complete and consistent description of the interfacial layer remains elusive. More recently, the theoretical frameworks and experimental data get complementary support from the simulation at a molecular level. This paper reviews the recent developments from the theoretical, experimental and simulation aspects. The combined results indicated that the interaction between hydration shells of adsorbed ions and the H-bonds network of surface water plays a critical role in the ionic adsorption. The factor should be incorporated into the conventional theories to correctly predict the ion distribution near the air/water surface.


Assuntos
Adsorção , Íons , Propriedades de Superfície , Água/química , Ar , Ligação de Hidrogênio
10.
Colloids Surf B Biointerfaces ; 181: 1035-1040, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31382331

RESUMO

Lipid rafts consisting mainly of sphingomyelin and cholesterol (Ch) on biomembrane surfaces are deeply related to cellular processes such as protein trafficking and signal transduction. During the processes, the raft microdomains affect the fluidty of biological membranes, which is controlled to large extents by Ch. In this paper, we have investigated the interaction between Ch and a semiflurinated alcohol (F6H9OH) from the aspect of a fluidty control using surface chemistry. The two-component Langmuir monolayer at the air-water interface was characterized by the surface pressure (π)-molecular area (A) and surface potential (ΔV)-A isotherms. The compressibility modulus and excess Gibbs free energy of mixing were calculated from the π-A isotherms. And also the two-dimensional phase diagram was constructed on the basis of phase transition pressures and monolayer collapse pressures. Furthermore, the phase behavior of binary monolayers was visualized with fluorescence microscopy (in situ) and atomic force microscopy (ex situ). The result here indicates a possibility of fluidity control of Ch-related membranes by arranging the fluorination degree of the constituent lipids.


Assuntos
Ar , Colesterol/química , Halogenação , Tensoativos/química , Água/química , Tamanho da Partícula , Propriedades de Superfície
11.
Colloids Surf B Biointerfaces ; 164: 1-10, 2018 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-29353162

RESUMO

The property of a newly synthesized tetrazine derivative comprised of double C18-saturated hydrocarbon chain (C18-rTz-C18) has been studied in situ at the air-water interface. C18-rTz-C18 or a gemini amphiphile contributes to restriction of its tetrazine moiety on the interface, which is expected to be used for bioimaging and analytical reagents. Herein, to understand lateral interactions between Tz and biomembrane constituents, we investigated the interfacial behavior of Langmuir monolayers composed of C18-rTz-C18 and biomembrane lipids such as DPPC, DPPG, DPPE, PSM, and Cholesterol (Ch). The lateral interaction of the binary monolayers was analyzed with the surface pressure (π)-molecular area (A) and surface potential (ΔV)-A isotherms. These thermodynamic data indicate that all of the two-components are miscible with each other. In particular, as opposed to the others, the monolayer stability of DPPE, which is a major constituent of the inner surface of cell membranes, is attenuated by the small-amount addition of C18-rTz-C18. This specific interaction implies the membrane destruction from the inside. The phase behavior during monolayer compression was visualized with Brewster angle microscopy (BAM), fluorescence microscopy (FM), and atomic force microscopy (AFM). The obtained morphologies exhibit a coexistence state of two different liquid-condensed domains derived from extra phospholipids and phospholipids-C18-rTz-C18 monolayers.


Assuntos
Compostos Heterocíclicos com 1 Anel/química , Lipídeos de Membrana/química , Tensoativos/química , 1,2-Dipalmitoilfosfatidilcolina/química , Microscopia de Força Atômica , Temperatura , Termodinâmica
12.
J Oleo Sci ; 66(5): 479-489, 2017 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-28381774

RESUMO

The interfacial behavior of binary monolayers of 1,1'-(1,ω-alkanediyl)-bispyridinium perfluorotetradecanecarboxylate (CnBP(FC14)2, n = 2, 6, or 10) and dipalmitoylphosphatidylcholine (DPPC) was studied using surface pressure (π)-area (A) and surface potential (ΔV)-A isotherm measurements and morphological observations. In a previous study, we showed that the spacer moieties of C2BP(FC14)2 and C6BP(FC14)2 are completely dissociated after spreading on 0.15 M NaCl, whereas the C10BP(FC14)2 spacer moieties do not dissociate in the monolayer state. However, in the present study, the ΔV-A isotherm indicated that the C10BP moiety partially dissociates in the presence of DPPC monolayers. The excess Gibbs free energy of mixing and two-dimensional phase diagrams suggest that CnBP(FC14)2 is miscible with DPPC monolayers and also has a fluidizing effect on DPPC monolayers. The phase behavior of the binary monolayers was observed with Brewster angle microscopy (BAM), fluorescence microscopy (FM), and atomic force microscopy (AFM). The dispersion mode of DPPC-rich domains by C10BP(FC14)2 is significantly different from those of the other CnBP(FC14)2 monolayers. These results suggest that the aliphatic chains in phospholipids control the dissociation of divalent spacers bound to fluorinated surfactants or amphiphiles.


Assuntos
1,2-Dipalmitoilfosfatidilcolina , Fluorocarbonos , Eletricidade Estática , Halogenação , Hidrocarbonetos , Microscopia de Força Atômica , Microscopia de Fluorescência , Transição de Fase , Fosfolipídeos , Cloreto de Sódio , Propriedades de Superfície , Tensoativos
13.
J Phys Chem B ; 120(48): 12279-12286, 2016 12 08.
Artigo em Inglês | MEDLINE | ID: mdl-27934226

RESUMO

This article investigates the surface chemistry properties of the ß-galactosidase monolayer at the air-subphase interface at the vicinity of its substrate, X-gal. We have demonstrated that the ß-galactosidase in the monolayer form remained active and performed hydrolysis of the X-gal in the subphase. We investigated the ß-galactosidase Langmuir monolayer in absence and presence of X-gal in the subphase of varying concentration of X-gal with the sodium chloride solution. It was found that the limiting molecular area as well as the collapse surface pressure kept on decreasing with the increasing concentration of X-gal. In accordance to the data obtained from the isotherm it was also found that ß-galactosidase forms a stable monolayer that does not aggregate at the air-subphase interface. The stability of the monolayer at the air-subphase interface was studied by using compression-decompression cycles with and without X-gal at varying concentration and different surface pressures. The infrared reflection-absorption spectroscopy (IRRAS) and Brewster angle microscopy (BAM) of ß-galactosidase Langmuir monolayer was also investigated for pure and mixed ß-galactosidase at the air-subphase.


Assuntos
Galactosídeos/química , Indóis/química , beta-Galactosidase/química , Ar , Galactosídeos/metabolismo , Indóis/metabolismo , Cloreto de Sódio/química , beta-Galactosidase/metabolismo
14.
Langmuir ; 32(48): 12842-12847, 2016 12 06.
Artigo em Inglês | MEDLINE | ID: mdl-27934519

RESUMO

The ionic state of an adsorbed gemini surfactant at the air/water interface was investigated using a combination of surface potential and surface tension data. The combined model was developed and successfully described the experimental data. The results verified the existence of three ionic states of the gemini surfactant in the interfacial zone. Furthermore, the model can quantify the adsorbed concentrations of these species. At low concentrations, the fully dissociated state dominates the adsorption. At high concentrations, the fully associated state dominates, accounting for up to 80% of the total adsorption. In the middle range, the adsorption is dominated by the partially associated state, which has a maximum percentage of 80% at a critical micelle concentration of 0.5. The variation in the ionic state is a unique characteristic of gemini surfactants, which can be the underlying mechanism for their advantages over conventional surfactants.

15.
Langmuir ; 32(26): 6591-9, 2016 07 05.
Artigo em Inglês | MEDLINE | ID: mdl-27280946

RESUMO

Tetrazine (Tz) is expected to be used for bioimaging and as an analytical reagent. It is known to react very fast with trans-cyclooctene under water in organic chemistry. Here, to understand the interaction between Tz and biomembrane constituents, we first investigated the interfacial behavior of a newly synthesized Tz derivative comprising a C18-saturated hydrocarbon chain (rTz-C18) using a Langmuir monolayer spread at the air-water interface. Surface pressure (π)-molecular area (A) and surface potential (ΔV)-A isotherms were measured for monolayers of rTz-C18 and biomembrane constituents such as dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylglycerol (DPPG), dipalmitoyl phosphatidylethanolamine (DPPE), palmitoyl sphingomyelin (PSM), and cholesterol (Ch). The lateral interaction between rTz-C18 and the lipids was thermodynamically elucidated from the excess Gibbs free energy of mixing and two-dimensional phase diagram. The binary monolayers except for the Ch system indicated high miscibility or affinity. In particular, rTz-C18 was found to interact more strongly with DPPE, which is a major constituent of the inner surface of cell membranes. The phase behavior and morphology upon monolayer compression were investigated by using Brewster angle microscopy (BAM), fluorescence microscopy (FM), and atomic force microscopy (AFM). The BAM and FM images of the DPPC/rTz-C18, DPPG/rTz-C18, and PSM/rTz-C18 systems exhibited a coexistence state of two different liquid-condensed domains derived mainly from monolayers of phospholipids and phospholipids-rTz-C18. From these morphological observations, it is worthy to note that rTz-C18 is possible to interact with a limited amount of the lipids except for DPPE.

16.
J Oleo Sci ; 65(2): 99-109, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26833282

RESUMO

Pulmonary surfactant (PS) preparations based mainly on bovine or porcine extracts are commonly administered to patients with neonatal respiratory distress syndrome (NRDS) for therapy. The preparations are sufficiently effective to treat NRDS; however, they are associated with a risk of infection and involve costly purification procedures to achieve batch-to-batch reproducibility. Therefore, we investigated the mechanism and interfacial behavior of synthetic PS preparations containing a mimicking peptide (KLLKLLLKLWLKLLKLLL, Hel 13-5). In particular, a hybrid PS formulation with fluorinated amphiphiles is reported from the perspective of surface chemistry. Fluorinated amphiphiles are characterized by exceptional chemical and biological inertness, high oxygen-dissolving capacity, low surface tension, excellent spreading ability, and high fluidity. These properties are superior to those for the corresponding hydrocarbon analogs. Indeed, a small amount of fluorinated long-chain alcohols enhances the effectiveness of the model PS preparation for in vitro pulmonary functions. Moreover, the mode of the improved efficacy differs depending on the hydrophobic chain length in the alcohols. For alcohols with a short fluorocarbon (FC) chain, the monolayer phase of the model PS preparation remains disordered (fluidization). However, the addition of alcohols containing a long FC chain reduces the disordered/ordered phase transition pressure and the growth of ordered domains of the monolayer (condensation). Furthermore, repeated compression-expansion isotherms of the monolayers, which can simulate respiration in the lung, suggest irreversible elimination of the short-FC alcohol into the subphase and enhancement of the squeeze-out phenomenon of certain PS components by solid-like monolayer formation induced by the long-FC alcohol. We demonstrated that fluorinated amphiphiles may be used as additives for synthetic or commercial PS preparations for RDS treatment.


Assuntos
Álcoois Graxos/química , Fluorocarbonos , Surfactantes Pulmonares/química , Surfactantes Pulmonares/síntese química , Síndrome do Desconforto Respiratório do Recém-Nascido/tratamento farmacológico , Fenômenos Químicos , Interações Hidrofóbicas e Hidrofílicas , Transição de Fase , Surfactantes Pulmonares/uso terapêutico
17.
J Oleo Sci ; 64(1): 1-8, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25742921

RESUMO

According to the conventional Gibbs adsorption model, which is a common assumption about the molecular concentration at surfaces, the adsorbed film of soluble amphiphiles is located at the air/solution interface just like Langmuir monolayer which is illustrated in many physical chemistry text books on "Colloid and Interface Science". According to many proofs of the experimental results here, the newer idea for the surface adsorption is confirmed and explained, which is quite different from the conventional Gibbs surface excess model at the air/solution interface.


Assuntos
Modelos Teóricos , Tensoativos , Adsorção , Ar , Etilenoglicol , Pirenos , Solubilidade , Soluções , Espectrometria de Fluorescência , Propriedades de Superfície , Termogravimetria , Lipossomas Unilamelares , Volatilização , Água
18.
Langmuir ; 31(1): 50-6, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25494904

RESUMO

Alcohols are a significant group of surfactants which have been employed extensively in industry to improve the interfacial effects. Recently, the change in surface potential (ΔV) of two isomeric hexanols, methyl isobutyl carbinol (MIBC) and 1-hexanol, was investigated by using an ionizing (241)Am electrode. It clearly showed the opposite effects between MIBC and 1-hexanol in the interfacial zone: one enhanced the presence of cations, whereas the other enhanced the presence of anions. This study employs molecular dynamics simulation to provide new insights into the interactions between alcohol molecules and ions as well as water at the molecular level. The results qualitatively agreed with the experimental data and verified the significance of MIBC branching structure on the molecular arrangement within the interfacial zone. The results also highlighted the role of the second water layer on the interfacial properties.


Assuntos
Ar , Álcoois/química , Simulação de Dinâmica Molecular , Sais/química , Adsorção , Conformação Molecular , Tensão Superficial , Água/química
19.
J Oleo Sci ; 63(12): 1333-49, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25409691

RESUMO

Lung surfactant is a complex mixture of lipid and protein, responsible for alveolar stability, becomes dysfunctional due to alteration of its structure and function by leaked serum materials in disease. Serum proteins, cholesterol and low density lipoprotein (LDL) were studied with bovine lipid extract surfactant (BLES) using Langmuir films, and bilayer dispersions using Raman spectroscopy. While small amount of cholesterol (10 wt%) and LDL did not significantly affect the adsorption and surface tension lowering properties of BLES. However serum lipids, whole serum as well as higher amounts of cholesterol, and LDL dramatically altered the surface properties of BLES films, as well as gel-fluid structures formed in such films observed using atomic force microscopy (AFM). Raman-spectroscopic studies revealed that serum proteins, LDL and excess cholesterol had fluidizing effects on BLES bilayers dispersion, monitored from the changes in hydrocarbon vibrational modes during gel-fluid thermal phase transitions. This study clearly suggests that patho-physiological amounts of serum lipids (and not proteins) significantly alter the molecular arrangement of surfactant in films and bilayers, and can be used to model lung disease.


Assuntos
Surfactantes Pulmonares/química , Adsorção , Proteínas Sanguíneas/análise , Colesterol/análise , Bicamadas Lipídicas/análise , Lipoproteínas LDL/análise , Fluidez de Membrana , Microscopia de Força Atômica , Estrutura Molecular , Transição de Fase , Análise Espectral Raman , Propriedades de Superfície
20.
J Oleo Sci ; 63(11): 1159-68, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25296574

RESUMO

Mammalian lungs are covered with lipid-protein complexes or pulmonary surfactants. In this work, which aimed towards the less expensive production of artificial pulmonary surfactants, we produced surfactants composed of egg yolk lecithin (eggPC), palmitic acid, and hexadecanol (= 0.30/0.35/0.35, mol/mol/mol ) containing different amounts of Hel 13-5 (NH2-KLLKLLLKLWLKLLKLLL-COOH) as a substitute for the proteins in native pulmonary surfactants. Surface pressure (π)-molecular area (A) and surface potential (DV)-A isotherms of the mixtures were measured via the Wilhelmy and ionizing (241)Am electrode methods, respectively. The interactions between the lipid components and Hel 13-5 led to variations in the surface pressure caused by the expulsion of fluid components from the surface. Furthermore, the π-A and DV-A isotherms featured large hysteresis loops for the surfactant that contained a small amount of Hel 13-5 during compression and successive expansion cycling. To elucidate the morphology, the phase behavior was visualized in situ at the air-water interface by means of fluorescence microscopy; the images suggested less effective interactions between Hel 13-5 and the unsaturated PC in eggPC despite the similarity of their monolayer properties.


Assuntos
Gema de Ovo , Lecitinas , Surfactantes Pulmonares/síntese química , Ar , Álcoois Graxos , Ácido Palmítico , Surfactantes Pulmonares/química , Propriedades de Superfície , Água
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