Red biases sex categorization of human bodies.

Color is associated with gender information (e.g., red-female). However, little has been known on the effect of color on sex recognition of human bodies. This study aimed to investigate whether the color red could influence the categorization of human bodies by sex, and the effect of contextual information. Visual stimuli were created using body silhouettes varying along the waist-to-hip ratio from female to male shapes. These stimuli were presented in conjunction with red, green, and gray colors, which were used either as body color (Experiment 1) or background color (Experiment 2). Participants were instructed to categorize the sex of the body stimuli as either male or female by pressing labeled keys. The results showed that when red was used as a body color, it induced a bias toward feminine body perception, while when used as a background color, it induced a bias toward masculine body perception. Thus, the color red influenced the sex categorization of human bodies, which being modulated by contextual information. These findings provided novel insights into the effect of contextual color cues in sex recognition of human bodies.

An automatic red-female association tested by the Stroop task.

Colors have been reported to be associated with genders (e.g., reddish color-females). Here, we investigated color-gender associations (red-female/green-male) among Japanese participants using two Stroop-word categorization tasks. Ten Japanese gendered words were chosen as visual stimuli. In Experiment 1 (N = 23), participants were instructed to indicate whether a target word presented in either red, green, or gray font color was a masculine or feminine word. Results showed a congruency effect of red-female association that red font color facilitated the categorization of feminine words and inhibited the categorization of masculine words compared to other colors. No significant effect of green-male association was observed. Experiment 2 (N = 23 newly recruited participants) examined whether the congruency effect of color-gender associations could bias perceptual font color categorization. Participants were asked to discriminate whether the font color, with low saturation, was red or green while ignoring the word's meaning. Results showed that participants responded faster and made fewer errors when categorizing red font colors for feminine words than masculine words. Additionally, a congruent effect of green-male association on performance accuracy was observed, although it did not affect response times. These results indicate the existence of an automatically activated red-female association, which can influence both conceptual gender categorization and perceptual color processing.

The evasive truth: do mere exposures at the subliminal and supraliminal levels drive the illusory truth effect?

The subjective truth of a statement is boosted by mere exposure to itself or a part of itself. This phenomenon is referred to as the illusory truth effect. We examined whether subliminal pre-exposure to the statement topic would increase its subjective truth. In the exposure phase, participants observed the topic, which was presented supraliminally or subliminally. After the exposure phase, they rated the subjective truth of the statement. If unconscious processing contributed to the illusory truth effect, subliminal exposure to the topic would increase the subjective truth of the statement. On the other hand, if the illusory truth effect required conscious and controlled processing, increases in the subjective truth of a statement would be induced only by supraliminal exposure to the topic. The results showed that the illusory truth effect was not found in either supraliminal or subliminal groups. Our findings provide no reliable evidence that pre-exposure to the statement topic saliently promotes its subjective truth.

Red background color biases gender categorization of human faces.

Color carries gender information (e.g., red-female). This study explored whether background color could influence the gender categorization of human faces. Visual stimuli were generated from faces whose sexually dimorphic content was morphed monotonically from female to male perception. The face stimulus was presented upright (Experiment 1) and inverted (Experiment 2) with three background colors (i.e., red, green, and gray). Participants were instructed to categorize the gender of the face stimulus as male or female by pressing one of two labelled keys. Results showed that a red background could bias the gender of an ambiguous upright face toward a female compared with green and gray background colors (Experiment 1). However, this red effect was diminished when the face stimulus was inverted (Experiment 2). These results suggest that red background color interacting with facial configuration features biases gender perception toward a female face, possibly through top-down processing of learned associations between the color red and femininity.

Revisiting the baby schema by a geometric morphometric analysis of infant facial characteristics across great apes.

Infants across species are thought to exhibit specific facial features (termed the "baby schema", such as a relatively bigger forehead and eyes, and protruding cheeks), with an adaptive function to induce caretaking behaviour from adults. There is abundant empirical evidence for this in humans, but, surprisingly, the existence of a baby schema in non-human animals has not been scientifically demonstrated. We investigated which facial characteristics are shared across infants in five species of great apes: humans, chimpanzees, bonobos, mountain gorillas, and Bornean orangutans. We analysed eight adult and infant faces for each species (80 images in total) using geometric morphometric analysis and machine learning. We found two principal components characterizing infant faces consistently observed across species. These included (1) relatively bigger eyes located lower in the face, (2) a rounder and vertically shorter face shape, and (3) an inverted triangular face shape. While these features are shared, human infant faces are unique in that the second characteristic (round face shape) is more pronounced, whereas the third (inverted triangular face shape) is less pronounced than other species. We also found some infantile features only found in some species. We discuss future directions to investigate the baby schema using an evolutionary approach.

Race categorization in noise.

People are typically faster to categorize the race of a face if it belongs to a race different from their own. This Other Race Categorization Advantage (ORCA) is thought to reflect an enhanced sensitivity to the visual race signals of other race faces, leading to faster response times. The current study investigated this sensitivity in a cross-cultural sample of Swiss and Japanese observers with a race categorization task using faces that had been parametrically degraded of visual structure, with normalized luminance and contrast. While Swiss observers exhibited an increasingly strong ORCA in both reaction time and accuracy as the face images were visually degraded up to 20% structural coherence, the Japanese observers manifested this pattern most distinctly when the faces were fully structurally-intact. Critically, for both observer groups, there was a clear accuracy effect at the 20% structural coherence level, indicating that the enhanced sensitivity to other race visual signals persists in significantly degraded stimuli. These results suggest that different cultural groups may rely on and extract distinct types of visual race signals during categorization, which may depend on the available visual information. Nevertheless, heavily degraded stimuli specifically favor the perception of other race faces, indicating that the visual system is tuned by experience and is sensitive to the detection of unfamiliar signals.

Concentration-Dependent Activity of Pazufloxacin against Pseudomonas aeruginosa: An In Vivo Pharmacokinetic/Pharmacodynamic Study.

The bacterium Pseudomonas aeruginosa is known to be associated with nosocomial infections around the world. Pazufloxacin, a potent DNA gyrase inhibitor, is known to be an effective drug candidate. However, it has not been clarified whether the pharmacokinetic (PK)/pharmacodynamic (PD) of pazufloxacin was effective against P. aeruginosa. Herein, we demonstrated that the PK/PD index of pazufloxacin against P. aeruginosa infection is used to optimize the dosing regiments. We constructed an in vivo infection model by infecting P. aeruginosa into the thigh of a mouse to determine the PD, and we measured the serum concentration of pazufloxacin to construct the PK model using high-performance liquid chromatography. The therapeutic efficacy of pazufloxacin was correlated with the ratio of the area under the free concentration time curve at 24 h to the minimum inhibitory concentration (fAUC24/MIC), and the maximum free concentration to the MIC (fCmax/MIC). Each contribution rate (R2) was 0.72 and 0.65, respectively, whereas the time at which the free drug concentration remained above the MIC (R2 = 0.28). The target value of pazufloxacin fAUC24/MIC for stasis was 46.1, for 1 log10 it was 63.8, and for 2 log10 it was 100.8. Moreover, fCmax/MIC for stasis was 5.5, for 1 log10 it was 7.1, and for 2 log10 it was 10.8. We demonstrated that the in vivo concentration-dependent activity of pazufloxacin was effective against the P. aeruginosa infection, and successfully made the PK/PD model sufficiently bactericidal. The PK/PD model will be beneficial in preventing the spread of nosocomial infections.

The spatio-temporal features of perceived-as-genuine and deliberate expressions.

Reading the genuineness of facial expressions is important for increasing the credibility of information conveyed by faces. However, it remains unclear which spatio-temporal characteristics of facial movements serve as critical cues to the perceived genuineness of facial expressions. This study focused on observable spatio-temporal differences between perceived-as-genuine and deliberate expressions of happiness and anger expressions. In this experiment, 89 Japanese participants were asked to judge the perceived genuineness of faces in videos showing happiness or anger expressions. To identify diagnostic facial cues to the perceived genuineness of the facial expressions, we analyzed a total of 128 face videos using an automated facial action detection system; thereby, moment-to-moment activations in facial action units were annotated, and nonnegative matrix factorization extracted sparse and meaningful components from all action units data. The results showed that genuineness judgments reduced when more spatial patterns were observed in facial expressions. As for the temporal features, the perceived-as-deliberate expressions of happiness generally had faster onsets to the peak than the perceived-as-genuine expressions of happiness. Moreover, opening the mouth negatively contributed to the perceived-as-genuine expressions, irrespective of the type of facial expressions. These findings provide the first evidence for dynamic facial cues to the perceived genuineness of happiness and anger expressions.

Computational Process of Sharing Emotion: An Authentic Information Perspective.

Although results of many psychology studies have shown that sharing emotion achieves dyadic interaction, no report has explained a study of the transmission of authentic information from emotional expressions that can strengthen perceivers. For this study, we used computational modeling, which is a multinomial processing tree, for formal quantification of the process of sharing emotion that emphasizes the perception of authentic information for expressers' feeling states from facial expressions. Results indicated that the ability to perceive authentic information of feeling states from a happy expression has a higher probability than the probability of judging authentic information from anger expressions. Next, happy facial expressions can activate both emotional elicitation and sharing emotion in perceivers, where emotional elicitation alone is working rather than sharing emotion for angry facial expressions. Third, parameters to detect anger experiences were found to be correlated positively with those of happiness. No robust correlation was found between the parameters extracted from this experiment task and questionnaire-measured emotional contagion, empathy, and social anxiety. Results of this study revealed the possibility that a new computational approach contributes to description of emotion sharing processes.

Impairment effect of infantile coloration on face discrimination in chimpanzees.

Impaired face recognition for certain face categories, such as faces of other species or other age class faces, is known in both humans and non-human primates. A previous study found that it is more difficult for chimpanzees to differentiate infant faces than adult faces. Infant faces of chimpanzees differ from adult faces in shape and colour, but the latter is especially a salient cue for chimpanzees. Therefore, impaired face differentiation of infant faces may be due to a specific colour. In the present study, we investigated which feature of infant faces has a greater effect on face identification difficulty. Adult chimpanzees were tested using a matching-to-sample task with four types of face stimuli whose shape and colour were manipulated as either infant or adult one independently. Chimpanzees' discrimination performance decreased as they matched faces with infant coloration, regardless of the shape. This study is the first to demonstrate the impairment effect of infantile coloration on face recognition in non-human primates, suggesting that the face recognition strategies of humans and chimpanzees overlap as both species show proficient face recognition for certain face colours.

Pharmacokinetic/pharmacodynamic evaluation of teicoplanin against Staphylococcus aureus in a murine thigh infection model.

OBJECTIVES: Pharmacokinetic/pharmacodynamic (PK/PD) analysis using murine infection models is a well-established methodology for optimising antimicrobial therapy. Therefore, we investigated the PK/PD indices of teicoplanin againstStaphylococcus aureus using a murine thigh infection model. METHODS: Mice were rendered neutropenic by administration of a two-step dosing of cyclophosphamide. Then, isolates of methicillin-susceptibleS. aureus (MSSA) or methicillin-resistant S. aureus (MRSA) were inoculated into the thighs of neutropenic mice. PK/PD analyses were performed by non-linear least-squared regression using the MULTI program. RESULTS: Target values offCmax/MIC (r2 = 0.94) of teicoplanin for static effect and 1 log10 kill against MSSA were 4.44 and 15.44, respectively. Target values of fAUC24/MIC (r2 = 0.92) of teicoplanin for static effect and 1 log10 kill against MSSA were 30.4 and 70.56, respectively. Target values of fCmax/MIC (r2 = 0.91) of teicoplanin for static effect and 1 log10 kill against MRSA were 8.92 and 14.16, respectively. Target values of fAUC24/MIC (r2 = 0.92) of teicoplanin for static effect and 1 log10 kill against MRSA were 54.8 and 76.4, respectively. CONCLUSION: These results suggest thatfCmax/MIC and fAUC24/MIC are useful PK/PD indices of teicoplanin against MSSA and MRSA.

A new data-driven mathematical model dissociates attractiveness from sexual dimorphism of human faces.

Human facial attractiveness is evaluated by using multiple cues. Among others, sexual dimorphism (i.e. masculinity for male faces/femininity for female faces) is an influential factor of perceived attractiveness. Since facial attractiveness is judged by incorporating sexually dimorphic traits as well as other cues, it is theoretically possible to dissociate sexual dimorphism from facial attractiveness. This study tested this by using a data-driven mathematical modelling approach. We first analysed the correlation between perceived masculinity/femininity and attractiveness ratings for 400 computer-generated male and female faces (Experiment 1) and found positive correlations between perceived femininity and attractiveness for both male and female faces. Using these results, we manipulated a set of faces along the attractiveness dimension while controlling for sexual dimorphism by orthogonalisation with data-driven mathematical models (Experiment 2). Our results revealed that perceived attractiveness and sexual dimorphism are dissociable, suggesting that there are as yet unidentified facial cues other than sexual dimorphism that contribute to facial attractiveness. Future studies can investigate the true preference of sexual dimorphism or the genuine effects of attractiveness by using well-controlled facial stimuli like those that this study generated. The findings will be of benefit to the further understanding of what makes a face attractive.

Geometric morphometric analysis of Japanese female facial shape in relation to psychological impression space.

Facial appearance has essential consequences in various social interactions. Previous studies have shown that although people can perceive a variety of impressions from a face, these impressions may form from a relatively small number of core dimensions in the psychological impression space (e.g., valence and dominance). However, few studies have thus far examined which facial shape features contribute to perceptions of the core trait impression dimensions for Asian female faces. This study aimed to identify the commonalities between various facial impressions of Japanese female faces and determine the facial shape components associated with such impressions by applying geometric morphometric (GMM) analysis. In Experiment 1 (Modeling study), Japanese female faces were evaluated in terms of 18 trait adjectives that are frequently used to describe facial appearance in daily life. We found that Japanese female facial appearance is indeed evaluated mainly on the valence and dominance dimensions. In Experiment 2 (Validation study), we confirmed that all the trait impressions were quantitatively manipulated by transforming the facial shape features associated with valence and dominance. Our results provide evidence that various facial impressions derived from these two underlying dimensions can be quantitatively manipulated by transforming facial shape using the GMM techniques.

Colour matters more than shape for chimpanzees' recognition of developmental face changes.

Social primates must recognise developmental stages of other conspecifics in order to behave appropriately. Infant faces have peculiar morphological characteristics-relatively large eyes, a small nose, and small mouth-known as baby schema. In addition, the infant faces of many primate species have unique skin coloration. However, it is unclear which features serve as critical cues for chimpanzees to recognise developmental changes in their faces. The present study aimed to investigate the relative contributions of facial shape and colour to age categorisation in chimpanzees. We used a symbolic matching-to-sample task in which chimpanzees were trained to discriminate between adult and infant faces. Then, we tested how their age category judgments transferred to a series of morphed faces which systematically differed in facial shape and colour. Statistical image quantification analysis revealed significant differences both in shape and colour between adult and infant faces. However, we found that facial coloration contributed to age categorisation in chimpanzees more than facial shape. Our results showed that chimpanzees use unique infantile facial coloration as a salient cue when discriminating between adult and infant faces. The display of their developmental stages through facial colour may help chimpanzees to induce appropriate behaviour from other individuals.

Data-driven mathematical model of East-Asian facial attractiveness: the relative contributions of shape and reflectance to attractiveness judgements.

Facial attractiveness is judged through a combination of multiple cues including morphology (facial shape) and skin properties (facial reflectance). While several studies have examined the way in which people in Western cultures judge facial attractiveness, there have been fewer investigations into non-Western attitudes. This is because stimuli that quantitatively vary the attractiveness of non-Western faces are rare. In the present study, we built a model of the attractiveness of East-Asian faces, judged by East-Asian observers. Therefore, 400 computer-generated East-Asian faces were created and attractiveness rating scores were collected from Japanese observers. Data-driven mathematical calculations were used to identify quantitative links between facial attractiveness and shape and reflectance properties, with no prior hypothesis. Results indicate that faces with larger eyes, smaller noses and brighter skin are judged as more attractive, regardless of the sex of the faces, possibly reflecting a general preference for femininity. Shape is shown to be a strong determinant of attractiveness for both male and female faces, while reflectance properties are less important in judging male facial attractiveness. Our model provides a tool to effectively produce East-Asian face stimuli that quantitatively varies attractiveness and can be used to elucidate visual processes related to attractiveness judgements.

Shear-Induced Migration of a Transmembrane Protein within a Vesicle.

Biomembranes feature phospholipid bilayers and serve as the interface between cells or organelles and the extracellular and/or cellular environment. Lipids can move freely throughout the membrane; the lipid bilayer behaves like a fluid. Such fluidity is important in terms of the actions of membrane transport proteins, which often mediate biological functions; membrane protein motion has attracted a great deal of attention. Because the proteins are small, diffusion phenomena are often in play, but flow-induced transport has rarely been addressed. Here, we used a dissipative particle dynamics approach to investigate flow-induced membrane protein transport. We analyzed the drift of a membrane protein located within a vesicle. Under the influence of shear flow, the protein gradually migrated toward the vorticity axis via a random walk, and the probability of retention around the axis was high. To understand the mechanism of protein migration, we varied both shear strength and protein size. Protein migration was induced by the balance between the drag and thermodynamic diffusion forces and could be represented by the Péclet number. These results improve our understanding of flow-induced membrane protein transport.

Preferential access to awareness of attractive faces in a breaking continuous flash suppression paradigm.

Facial attractiveness is a core facial attribute in social interactions. This study used a breaking continuous flash suppression (b-CFS) paradigm to investigate whether facial attractiveness can be processed unconsciously. In the b-CFS paradigm, a monocularly viewed visual stimulus is erased from visual awareness by rapidly flashing, high-contrast masks presented to the other eye. Faces with different levels of attractiveness but an emotionally neutral expression were presented under CFS, and the time taken to break CFS was measured. Our results demonstrated that attractive faces were detected more quickly than unattractive ones, indicating privileged processing of attractive faces (Experiment 1). This effect dissipated when facial images were scrambled to disrupt the face-like configuration (Experiment 2) but was still observed in cases of inverted faces (Experiment 3). These results indicate that preconscious processing of facial attractiveness requires a face-like configuration but does not necessarily require information about precise metrical relationships between facial features.

Prioritized Identification of Attractive and Romantic Partner Faces in Rapid Serial Visual Presentation.

People are sensitive to facial attractiveness because it is an important biological and social signal. As such, our perceptual and attentional system seems biased toward attractive faces. We tested whether attractive faces capture attention and enhance memory access in an involuntary manner using a dual-task rapid serial visual presentation (dtRSVP) paradigm, wherein multiple faces were successively presented for 120 ms. In Experiment 1, participants (N = 26) were required to identify two female faces embedded in a stream of animal faces as distractors. The results revealed that identification of the second female target (T2) was better when it was attractive compared to neutral or unattractive. In Experiment 2, we investigated whether perceived attractiveness affects T2 identification (N = 27). To this end, we performed another dtRSVP task involving participants in a romantic partnership with the opposite sex, wherein T2 was their romantic partner's face. The results demonstrated that a romantic partner's face was correctly identified more often than was the face of a friend or unknown person. Furthermore, the greater the intensity of passionate love participants felt for their partner (as measured by the Passionate Love Scale), the more often they correctly identified their partner's face. Our experiments indicate that attractive and romantic partners' faces facilitate the identification of the faces in an involuntary manner.

Transcranial Direct Current Stimulation over the Medial Prefrontal Cortex and Left Primary Motor Cortex (mPFC-lPMC) Affects Subjective Beauty but Not Ugliness.

Neuroaesthetics has been searching for the neural bases of the subjective experience of beauty. It has been demonstrated that neural activities in the medial prefrontal cortex (mPFC) and the left primary motor cortex (lPMC) correlate with the subjective experience of beauty. Although beauty and ugliness seem to be semantically and conceptually opposite, it is still unknown whether these two evaluations represent extreme opposites in unitary or bivariate dimensions. In this study, we applied transcranial direct current stimulation (tDCS) to examine whether non-invasive brain stimulation modulates two types of esthetic evaluation; evaluating beauty and ugliness. Participants rated the subjective beauty and ugliness of abstract paintings before and after the application of tDCS. Application of cathodal tDCS over the mPFC with anode electrode over the lPMC, which induced temporal inhibition of neural excitability of the mPFC, led to a decrease in beauty ratings but not ugliness ratings. There were no changes in ratings of both beauty and ugliness when applying anodal tDCS or sham stimulation over the mPFC. Results from our experiment indicate that the mPFC and the lPMC have a causal role in generating the subjective experience of beauty, with beauty and ugliness evaluations constituting two distinct dimensions.