Assuntos
Doença de Bowen/etiologia , Dermatite Atópica/tratamento farmacológico , Glucocorticoides/uso terapêutico , Papillomavirus Humano 16/isolamento & purificação , Infecções por Papillomavirus/complicações , Neoplasias Cutâneas/etiologia , Administração Cutânea , Doença de Bowen/patologia , Doença de Bowen/cirurgia , Glucocorticoides/administração & dosagem , Humanos , Masculino , Pessoa de Meia-Idade , Pescoço , Pomadas , Infecções por Papillomavirus/virologia , Reação em Cadeia da Polimerase , Neoplasias Cutâneas/patologia , Neoplasias Cutâneas/cirurgiaRESUMO
Skin tumors are supposed to develop through accumulations of genetic and/or epigenetic events in normal cells of the skin. Among them, we focus on common skin tumors, including benign, seborrheic keratosis, and malignant, squamous cell carcinoma and melanoma. Many important molecules have been detected on the molecular tumorigenesis of each of them to date, and some drugs targeted for their molecules have been already developed. We review updates on the molecular tumorigenesis of these tumors with our current works.
Assuntos
Carcinogênese/patologia , Neoplasias Cutâneas/patologia , Pele/patologia , Carcinogênese/genética , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patologia , Epigênese Genética/genética , Humanos , Melanoma/genética , Melanoma/patologia , Neoplasias Cutâneas/genéticaRESUMO
The effects of long-term exposure of primary cultured rat dorsal root ganglion (DRG) cells to bradykinin (BK), compared to short-term exposure, were investigated to establish whether BK could induce prostaglandin E2 (PGE2) release from DRG cells. Short-term exposure (30 min) resulted in a small but significant amount of PGE2 release which was mainly inhibited by a selective COX-1 inhibitor, SC-560 but only partially by a selective COX-2 inhibitor, NS-398, and did not induce COX-2 protein as determined by Western blotting. In contrast, long-term exposure (3 h) induced a large amount of PGE2 release, which was completely abolished by indomethacin or NS-398. The level of COX-2 mRNA began to be detected by ribonuclease protection assay after 30 min of 100 nM BK exposure, maintained maximal expression for 1 h, and subsequently declined to the basal level. The level of COX-2 protein was expressed to follow the time course of COX-2 mRNA induction by BK in a delayed but similar kinetic manner. The expression of COX-2 induced by BK in DRG cells was inhibited by a BK B2 receptor antagonist, HOE140, but not a B1 receptor antagonist, Lys-des-Arg9, (Leu8)-BK. Thus, BK has been shown to induce COX-2 protein by B2 receptor, which may cause prostanoid generation in rat DRG cells, which may play an important role in the pathogenesis of inflammatory pain and hyperalgesia around the primary sensory neurons.
