RESUMO
Difficulties in repairing the defects of the teeth are related with allergic-inflammatory, traumatic and dystrophic complications arising from the interaction of the foreign body with the mucous tissues of the oral cavity after the patient's prosthesis is established. The aim of our study was to establish the toxic pro-inflammatory activity of materials used for the manufacturing of bases of removable dentures - plastics based on polymethylmethacrylate prosthetic complexes Prothyl Hot, Ftorax and Perflex Flexi Nylon on the model of human leukemia transformed T cells (Jurkat cells) and MDCK cells. For the cells simulation Jurkat and MDCK cells was incubated with the components of prosthetic materials, Prothyl Hot, Ftorax and Perflex Flexi Nylon/ Prosthetic materials were added to the incubation medium at the doses used in practice (calculated at 106 cells); duration of incubation was 24 hours. A comparative assessment of the toxicity of prosthetic materials was determined by the MTT test (activity of mitochondrial dehydrogenases). Statistical analysis was carried out using the package (SPSS version 11.0). The statistical reliability of the difference between the indices was evaluated by the Student t test (the P <0.05 level was considered reliable). The results of the conducted studies testify to the absence of toxicity of the complexes Prothyl Hot, Ftorax and Perflex Flexi Nylon, used as a basis of circuit prostheses, on intact Jurkat and MDCK cells, as evidenced by the stability of their mitochondrial dehydrogenases. Based on the analysis of the conducted studies, it can be concluded, that as Jurkat and MDCK cells are used as models of immune and epithelial cells, the materials used for manufacturing of removable prostheses, the polymethylmethacrylate-based plastics (Prothyl Hot and Ftorax) and elastic thermoplastic polymer material Perflex Flexi Nylon, are not toxic. Studied materials, with the high probability, are not capable to cause massive death of immune cells, development of allergic or inflammatory damages, which in turn can stat cause the development of stomatitis and gingivitis, the destruction of the paradental tissue.
Assuntos
Materiais Dentários/toxicidade , Polimetil Metacrilato/toxicidade , Animais , Dentaduras , Cães , Humanos , Células Jurkat , Células Madin Darby de Rim CaninoRESUMO
The purpose of our study was a comparative analysis of the effect of dentures from various materials on the immunological and redox-dependent homeostasis of the oral cavity. We studied 60 patients with removable dentures made based on plastics Prothyl Hot, Vertex BasiQ 20 (differing by polymerization regime) and elastic thermoplastic polymer Perflex Flexi Nylon. The control group consisted of 15 volunteers with a practically healthy oral cavity, who did not use dentures. Saliva collected on an empty stomach in a glass tube without the use of a stimulator before the establishment of a denture and 3 days and 1 month after. The content of the protein P-53 in saliva determined by immunoenzymatic assay with use of "Cusabio" reagent. The cytokines (IL1ß, IL10) content in saliva was determined immunoenzymatic assay. To determine the redox balance in the saliva of patients, the lipoperoxydradicals content (LOO.) content (by EPR method, using the spin-labeled α-phenyl-tertbutylnitron (PBN) (SIGMA)) and the activity of antioxidant enzymes (catalase and SOD) (by spectrophotometry) studied. Statistical processing of the results was carried out using the software package SPSS (version 10.0). Results of analysis show that defects associated with a lack of teeth do not affect the immune and oxidative balance of the oral cavity, but contribute to the development of destructive changes in the oral cavity's soft tissues, which manifested by an increase in the content of the proapoptotic protein P-53 in the saliva. After establishment of a denture, the intensity of apoptosis in the oral cavity tissues reduced. Establishment of a denture induced development of an inflammatory reaction during the first days, the intensity of which gradually decreased and completely disappeared at the end of the first month of the observation (manifested by the normalization of the parameters of the immune balance and antioxidant system). Minimal traumatic effects observed during establishment of a denture made based on Perflex Flexi Nylon.
Assuntos
Materiais Dentários/uso terapêutico , Prótese Parcial Removível , Regulação da Expressão Gênica/efeitos dos fármacos , Resinas Sintéticas/uso terapêutico , Saliva/efeitos dos fármacos , Estudos de Casos e Controles , Catalase/genética , Catalase/metabolismo , Planejamento de Dentadura , Humanos , Interleucina-10/agonistas , Interleucina-10/genética , Interleucina-10/metabolismo , Interleucina-1beta/agonistas , Interleucina-1beta/genética , Interleucina-1beta/metabolismo , Peróxidos Lipídicos/agonistas , Peróxidos Lipídicos/metabolismo , Boca/metabolismo , Boca/cirurgia , Oxirredução/efeitos dos fármacos , Saliva/química , Saliva/metabolismo , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismo , Proteína Supressora de Tumor p53/agonistas , Proteína Supressora de Tumor p53/genética , Proteína Supressora de Tumor p53/metabolismoRESUMO
The aim of our study was the investigation of toxicity of denture prosthetic appliance Prothyl Hot on the model system of Jurkat cell culture. As a result of our study it was revealed that denture prosthetic appliance Prothyl Hot: - hadn't manifested toxic effects the viability Jurkat cells (that reviled by stability of activity of mitochondrial dehydrogenises and mean of mitochondrial membrane potential; - didn't influence on the balance of pro-and antyinflammatory cytokines, expressed by Jurkat cells; - induces intensification of oxidative metabolism in Jurkat cells, which may be considered as compensatory reaction developing in the cells.
