RESUMO
OBJECTIVE: To investigate the possible effects of nuclear factor-KappaB (NF-KappaB) activation on the expression of intercellular adhesion molecular-1 (ICAM-1) and P-selectin in murine acute lung injury (ALI) and assess the potential beneficial effects and mechanism of low molecular weight heparin (LMWH) and aspirin (ASA). METHODS: Rat ALI model was reproduced by injection of lipopolysaccharide into tail vein. Sixty rats were divided randomly into four groups (n=15): normal control group, ALI group, LMWH group and ASA group. The change in NF-KappaB activity in the lung to show its relation with ICAM-1 and P-selectin was observed by immunohistochemistry. RESULTS: Compared with normal control group, the activity of NF-KappaB and the expression of ICAM-1 and P-selectin increased significantly in the lung tissue of ALI group (all P<0.05). Compared with ALI group, the level of NF-KappaB activity and the expression of ICAM-1 and P-selectin were obviously down regulated, and also the pathological lesion and inflammatory response of lung were improved in LMWH and ASA groups. However, the therapeutic effects of ASA were stronger than those of LMWH (all P<0.05). CONCLUSION: NF-KappaB activation plays an important role in ALI. NF-KappaB takes part in the activation of many kinds of inflammatory cells such as neutrophil, endothelial cells and so on, and it adjusts the genetic expression of ICAM-1 and P-selectin. LMWH and ASA show their beneficial effects on lung injury in both functional and morphological aspects. But the mechanism is different. LMWH can indirectly inhibit the activation of NF-KappaB, improve lung microcirculation and decrease the adhesion of neutrophil and platelet. ASA plays the role as an inhibitor of NF-KappaB activation.
