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INTRODUCTION: In routine tissue processing, formalin has been proved as efficient as fixative since inception and hazards associated with it are of major safety and health concern. AIM: The aim of this study is to compare the efficacy of natural fixatives such as jaggery and Khandsari over formalin. MATERIALS AND METHODS: Ninety normal tissue specimen collected during minor oral surgical procedures were included in this study. Thirty specimen each were fixed in 30% jaggery (Group-A), 30% Khandsari (Group-B) and 10% formalin (Group-C). The slides obtained after tissue processing were analyzed for the quality of fixation. The tissue sections were assessed for cellular outline, cytoplasmic details, nuclear details, staining quality and overall morphology. Each criterion was rated on a scale of 1-4. (one for poor and four for excellent). The study was double-blinded and subjected to the Kruskal-Wallis ANOVA and Chi-square test. RESULTS: The cellular outline is excellent in 90% (Group-C) followed by 36.67% (Group-B) of specimens. With respect to cytoplasmic staining 83.33% (Group-C) of tissues showed excellent results followed by 60% (Group-B), 33.33% (Group-A). Nuclear details are excellent in 86.67% (Group-C) followed by 83.33% (Group-B), 36.67% (Group-A) of specimens. With respect to staining quality 93.33% (Group-C) followed by 50% (Group-B), 26.67% (Group-A). Overall morphology is excellent in 90% (Group-C) followed by 46.67% (Group-B). CONCLUSION: In the present study, Khandsari was on par with formalin and our effort of using Khandsari and jaggery for tissue fixation in human oral tissues yielded good results.
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Ameloblastomas are benign epithelial odontogenic neoplasms which are locally aggressive with an insidious growth pattern. Based on the clinical, radiographic, histopathology and behavioral and prognostic aspects, four variants of ameloblastoma are distinguished. They include solid/multicystic ameloblastoma, unicystic ameloblastoma (UA), peripheral ameloblastoma (PA) and desmoplastic ameloblastoma. UA and PA are two variants that have no clinical or radiological resemblance to each other. A case of simultaneous occurrence of these two lesions displaying an array of different histopathological entities together, with a note on pathological insight, has been reported in a 59-year-old adult male patient.
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INTRODUCTION: Age estimation is one of the essential factors in establishing the identity of an individual. Among various methods, exfoliative cytology (EC) is a unique, noninvasive technique, involving simple, and pain-free collection of intact cells from the oral cavity for microscopic examination. OBJECTIVE: The study was undertaken with an aim to estimate the age of an individual from the average cell size of their buccal smears calculated using image analysis morphometric software and the pulp-tooth area ratio in mandibular canine of the same individual using radiovisiography (RVG). MATERIALS AND METHODS: Buccal smears were collected from 100 apparently healthy individuals. After fixation in 95% alcohol, the smears were stained using Papanicolaou stain. The average cell size was measured using image analysis software (Image-Pro Insight 8.0). The RVG images of mandibular canines were obtained, pulp and tooth areas were traced using AutoCAD 2010 software, and area ratio was calculated. The estimated age was then calculated using regression analysis. RESULTS: The paired t-test between chronological age and estimated age by cell size and pulp-tooth area ratio was statistically nonsignificant (P > 0.05). CONCLUSION: In the present study, age estimated by pulp-tooth area ratio and EC yielded good results.
