Primary Metabolic Response of Aristolochia contorta to Simulated Specialist Herbivory under Elevated CO2 Conditions.

This study explores how elevated carbon dioxide (CO2) levels affects the growth and defense mechanisms of plants. We focused on Aristolochia contorta Bunge (Aristolochiaceae), a wild plant that exhibits growth reduction under elevated CO2 in the previous study. The plant has Sericinus montela Gray (Papilionidae) as a specialist herbivore. By analyzing primary metabolites, understanding both the growth and defense response of plants to herbivory under elevated CO2 conditions is possible. The experiment was conducted across four groups, combining two CO2 concentration conditions (ambient CO2 and elevated CO2) with two herbivory conditions (herbivory treated and untreated). Although many plants exhibit increased growth under elevated CO2 levels, A. contorta exhibited reduced growth with lower height, dry weight, and total leaf area. Under herbivory, A. contorta triggered both localized and systemic responses. More primary metabolites exhibited significant differences due to herbivory treatment in systemic tissue than local leaves that herbivory was directly treated. Herbivory under elevated CO2 level triggered more significant responses in primary metabolites (17 metabolites) than herbivory under ambient CO2 conditions (five metabolites). Several defense-related metabolites exhibited higher concentrations in the roots and lower concentrations in the leaves in response to the herbivory treatment in the elevated CO2 group. This suggests a potential intensification of defensive responses in the underground parts of the plant under elevated CO2 levels. Our findings underscore the importance of considering both abiotic and biotic factors in understanding plant responses to environmental changes. The adaptive strategies of A. contorta suggest a complex response mechanism to elevated CO2 and herbivory pressures.

Environmentally adaptive reshaping of plant photomorphogenesis by karrikin and strigolactone signaling.

Coordinated morphogenic adaptation of growing plants is critical for their survival and propagation under fluctuating environments. Plant morphogenic responses to light and warm temperatures, termed photomorphogenesis and thermomorphogenesis, respectively, have been extensively studied in recent decades. During photomorphogenesis, plants actively reshape their growth and developmental patterns to cope with changes in light regimes. Accordingly, photomorphogenesis is closely associated with diverse growth hormonal cues. Notably, accumulating evidence indicates that light-directed morphogenesis is profoundly affected by two recently identified phytochemicals, karrikins (KARs) and strigolactones (SLs). KARs and SLs are structurally related butenolides acting as signaling molecules during a variety of developmental steps, including seed germination. Their receptors and signaling mediators have been identified, and associated working mechanisms have been explored using gene-deficient mutants in various plant species. Of particular interest is that the KAR and SL signaling pathways play important roles in environmental responses, among which their linkages with photomorphogenesis are most comprehensively studied during seedling establishment. In this review, we focus on how the phytochemical and light signals converge on the optimization of morphogenic fitness. We also discuss molecular mechanisms underlying the signaling crosstalks with an aim of developing potential ways to improve crop productivity under climate changes.

Age-dependent resistance of a perennial herb, Aristolochia contorta against specialist and generalist leaf-chewing herbivores.

Plants need to balance investments in growth and defense throughout their life to increase their fitness. To optimize fitness, levels of defense against herbivores in perennial plants may vary according to plant age and season. However, secondary plant metabolites often have a detrimental effect on generalist herbivores, while many specialists have developed resistance to them. Therefore, varying levels of defensive secondary metabolites depending on plant age and season may have different effects on the performance of specialist and generalist herbivores colonizing the same host plants. In this study, we analyzed concentrations of defensive secondary metabolites (aristolochic acids) and the nutritional value (C/N ratios) of 1st-, 2nd- and 3rd-year Aristolochia contorta in July (the middle of growing season) and September (the end of growing season). We further assessed their effects on the performances of the specialist herbivore Sericinus montela (Lepidoptera: Papilionidae) and the generalist herbivore Spodoptera exigua (Lepidoptera: Noctuidae). Leaves of 1st-year A. contorta contained significantly higher concentrations of aristolochic acids than those of older plants, with concentrations tending to decrease over the first-year season. Therefore, when first year leaves were fed in July, all larvae of S. exigua died and S. montela showed the lowest growth rate compared to older leaves fed in July. However, the nutritional quality of A. contorta leaves was lower in September than July irrespective of plant age, which was reflected in lower larval performance of both herbivores in September. These results suggest that A. contorta invests in the chemical defenses of leaves especially at a young age, while the low nutritional value of leaves seems to limit the performance of leaf-chewing herbivores at the end of the season, regardless of plant age.

Ontogeny-dependent effects of elevated CO2 and watering frequency on interaction between Aristolochia contorta and its herbivores.

Effects of environmental change on plants can differ due to sequential changes in their life-history strategies (i.e., ontogenetic variations). The fitness of herbivorous insects by physiological changes of the host plant could be affected depending on their diet breadth. However, little is known regarding the combinational effects of plant ontogeny and climate change on plant-herbivore interactions. This study examined the plant ontogeny-dependent effects of climate change on the interaction between a host plant (Aristolochia contorta), its specialist herbivore (Sericinus montela), and a generalist herbivore (Spodoptera exigua). Plants were grown under a factorial design of two distinct CO2 concentrations (ambient, 400 ppm; elevated, 560 ppm) and two watering frequencies (control, once a week; increased, twice a week). Plant ontogeny ameliorated the effects of climate change by altering its defensive traits, where nutrient-related factors were cumulatively affected by climate change. Herbivore performance was assessed at three different plant ontogenetic stages (1st-year juvenile, 1st-year senescence, and 2nd-year juvenile). Elevated CO2 levels reduced the growth and survival of the specialist herbivore, whereas increased watering frequency partially alleviated this reduced performance. Generalist herbivore performance slightly increased under elevated CO2 levels with progressing ontogenetic stages. The effects of climate change, both elevated CO2 and increased watering frequency were weaker in 2nd-year juveniles than in 1st-year juveniles. Elevated CO2 levels detrimentally affected the nutritional quality of A. contorta leaves. The effects of climate change on both specialist and generalist herbivore performance differed as plant ontogenetic stage proceeded. Increased growth rates and survival of the generalist herbivore at the latter ontogenetic stage might negatively affect the population dynamics of a specialist herbivore. This study suggests that biases are possible when the plant-herbivore interaction under a changing environment is predicted from a singular plant ontogenetic stage.

Reduced host plant growth and increased tyrosine-derived secondary metabolites under climate change and negative consequences on its specialist herbivore.

Compositive changes in climatic factors, e.g., carbon dioxide (CO2) and precipitation frequency and intensity, affect the strength of species interactions via responses in plants. Therefore, understanding the effects of climate change on plant-herbivore interactions is important to maintain biodiversity as about 70% of insects are herbivorous. However, the interactive effects of CO2 and precipitation on plants and consequences for herbivores are poorly understood. Here, we examined how elevated CO2 and increased watering frequency affect the growth and resistance responses of Aristolochia contorta and the growth performance of its specialist herbivore, Sericinus montela. Elevated CO2 suppressed growth with decreased photosynthesis ability, and increased resistance in plants. In contrast, increased watering frequency partly ameliorated the negative effects of high CO2. Growth performance of specialist herbivores decreased under elevated CO2 condition as a consequence of increased resistance in plants. Due to the significant effects of CO2, we suggest that both the quantity and the quality of host plants as a food would decline, and the growth performance of its specialist herbivore might be threatened as climate change progresses.

Flowering season of vernal herbs is shortened at elevated temperatures with reduced precipitation in early spring.

Vernal herbs are exposed to the risk of climate change under spring frost and canopy closure. Although vernal herbs contribute to the biodiversity of the understorey layer in temperate forests, few studies assessed the effect of climate change on the phenology of the herbs. To examine phenological shifts in flowering seasons of vernal herb species caused by climate change, a greenhouse experiment was conducted using four species (Adonis amurensis, Hepatica nobilis var. japonica, Viola phalacrocarpa, and Pulsatilla cernua) under two temperature conditions (ambient or elevated temperature) and two precipitation conditions (convective or reduced precipitation). Experimental warming advanced overall aspects of the flowering timing including the first and last day of flowering. The growth of flowering stalk was also promoted by elevated temperature. Effects of decreased precipitation varied among species, which advanced the last day of the flowering of the later flowering species. Consequently, a decrease in overall flowering period length was observed. These results indicate that overall, climate change results in a shortening of the flowering season of vernal herb species, specifically at a higher temperature and under conditions of less precipitation.

Auxin mediates the touch-induced mechanical stimulation of adventitious root formation under windy conditions in Brachypodium distachyon.

BACKGROUND: It is widely perceived that mechanical or thigmomorphogenic stimuli, such as rubbing and bending by passing animals, wind, raindrop, and flooding, broadly influence plant growth and developmental patterning. In particular, wind-driven mechanical stimulation is known to induce the incidence of radial expansion and shorter and stockier statue. Wind stimulation also affects the adaptive propagation of the root system in various plant species. However, it is unknown how plants sense and transmit the wind-derived mechanical signals to launch appropriate responses, leading to the wind-adaptive root growth. RESULTS: Here, we found that Brachypodium distachyon, a model grass widely used for studies on bioenergy crops and cereals, efficiently adapts to wind-mediated lodging stress by forming adventitious roots (ARs) from nonroot tissues. Experimental dissection of wind stimuli revealed that not bending of the mesocotyls but physical contact of the leaf nodes with soil particles triggers the transcriptional induction of a group of potential auxin-responsive genes encoding WUSCHEL RELATED HOMEOBOX and LATERAL ORGAN BOUNDARIES DOMAIN transcription factors, which are likely to be involved in the induction of AR formation. CONCLUSIONS: Our findings would contribute to further understanding molecular mechanisms governing the initiation and development of ARs, which will be applicable to crop agriculture in extreme wind climates.