RESUMO
PURPOSE: This study explored the relationships of maturity fears and appearance interest(interest in appearance, interest in weight) with self-concept among girls with precocious puberty. METHODS: The participants for this study were 120 elementary school students (8 to 10 years old) and their parents from two hospitals located in Chungcheong Province. Data were collected from January to February 2019 using self-reported questionnaires. The collected data were analyzed using descriptive statistics, the independent t-test, the Mann-Whitney U test, one-way analysis of variance, Pearson correlation coefficients, and multiple regression analysis in SPSS for Windows version 23.0. RESULTS: Statistically significant negative correlations were found between self-concept and maturity fears (r=-.34, p<.001) and interest in weight (r=-.29, p=.001). Maturity fears were identified as a factor affecting the self-concept of girls with precocious puberty (ß=-.34, p<.001). This variable explained 11.5% of the variance in the self-concept of girls who experienced precocious puberty. CONCLUSION: This study confirmed that maturity fears had a major influence on self-concept in girls with precocious puberty. The results of this study can be used as basic data for the development of a psychosocial nursing intervention program for girls who experience precocious puberty.
RESUMO
Acquired resistance to tamoxifen (TAM) is a serious therapeutic problem in breast cancer patients. We have shown that Pin1, a peptidyl prolyl isomerase, is consistently overexpressed in TAM-resistant MCF-7 cells (TAMR-MCF-7 cells) and plays a key role in the enhanced angiogenic potential of TAMR-MCF-7 cells. In the present study, we focused on signaling pathways for Pin1 up-regulation in TAMR-MCF-7 cells. Relative to MCF-7 cells, Pin1 gene transcription and E2 transcription factor1 (E2F1) expression were enhanced in TAMR-MCF-7 cells. E2F1 siRNA significantly reduced both the protein expression and the promoter transcriptional activity of Pin1. Activities of phosphatidylinositol 3-kinase (PI3K), extracellular signal-regulated kinase (ERK) and p38 kinase were all higher in TAMR-MCF-7 cells than in control MCF-7 cells and the enhanced Pin1 and E2F1 expression in TAMR-MCF-7 cells was reversed by inhibition of PI3K or p38 kinase. Moreover, the higher production of vascular endothelial growth factor (VEGF) in TAMR-MCF-7 cells was significantly diminished by suppression of PI3K or p38 kinase. These results suggest that Pin1 overexpression and subsequent VEGF production in TAMR-MCF-7 cells are mediated through PI3-kinase or p38 kinase-dependent E2F1 activation.
Assuntos
Neoplasias da Mama , Resistencia a Medicamentos Antineoplásicos , Fator de Transcrição E2F1/metabolismo , Regulação Neoplásica da Expressão Gênica , Peptidilprolil Isomerase/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Tamoxifeno/farmacologia , Fator A de Crescimento do Endotélio Vascular/metabolismo , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo , Antineoplásicos Hormonais/farmacologia , Antineoplásicos Hormonais/uso terapêutico , Western Blotting , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/genética , Neoplasias da Mama/metabolismo , Linhagem Celular Tumoral , Fator de Transcrição E2F1/genética , Ensaio de Imunoadsorção Enzimática , Feminino , Inativação Gênica/efeitos dos fármacos , Genes Reporter , Humanos , Luciferases/análise , Peptidilprolil Isomerase de Interação com NIMA , Peptidilprolil Isomerase/genética , Fosfatidilinositol 3-Quinases/genética , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/farmacologia , Transdução de Sinais , Tamoxifeno/uso terapêutico , Regulação para Cima , Fator A de Crescimento do Endotélio Vascular/genética , Proteínas Quinases p38 Ativadas por Mitógeno/genéticaRESUMO
An optical CO gas sensor was investigated using cobalt oxide thin films prepared by pulsed laser deposition. The cobalt oxide films were deposited on quartz glass and silicon wafer substrates in Ar at 0.07-133 Pa. The morphology and crystal phase of the films were changed by Ar pressure. Sensitivity was estimated as the transmittance change of the film in dry air and at 200 ppm of CO gas ambient at 350 degrees C. The morphology of the films greatly affected the sensing properties. The optimum Ar pressure for cobalt oxide film preparation for CO gas sensing was suggested to be 13.3 Pa, based on the relationship between the morphology and the optical sensor properties of the films.
