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1.
Biochim Biophys Acta ; 1830(6): 3656-63, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23416061

RESUMO

BACKGROUND: Diabetes mellitus is characterized by high blood glucose levels. Pancreatic ß cell death contributes to type 1 and type 2 diabetes. Akita mice, which harbor a human permanent neonatal diabetes-linked mutation (Cys96Tyr) in the insulin gene, are well established as an animal model of diabetes caused by pancreatic ß cell exhaustion. Mutant Insulin 2 protein (Ins2(C96Y)) induces endoplasmic reticulum (ER) stress and pancreatic ß cell death in Akita mice, although the molecular mechanism of Ins(C96Y)-induced cell death remains unclear. METHODS: We investigate the mechanisms of Ins2(C96Y)-induced pancreatic ß cell death in vitro and in vivo, using p38 inhibitor (SB203580), MIN6 cell (pancreatic ß cell line), Akita mice and apoptosis signal-regulating kinase 1 (ASK1) knockout mice. RESULTS: The expression of Ins(C96Y) activated the ASK1-p38 pathway. Deletion of ASK1 mitigated Ins(C96Y)-induced pancreatic ß cell death and delayed the onset of diabetes in Akita mice. Moreover, p38 inhibitor suppressed Ins(C96Y)-induced MIN6 cell death. CONCLUSIONS: These findings suggest that ER stress-induced ASK1-p38 activation, which is triggered by the accumulation of Ins(C96Y), plays an important role in the pathogenesis of diabetes. GENERAL SIGNIFICANCE: Pancreatic ß cell death caused by insulin overload appears to be involved in the pathogenesis of type 1 and type 2 diabetes. Inhibition of the ASK1-p38 pathway may be an effective therapy for various types of diabetes.


Assuntos
Diabetes Mellitus Tipo 1/enzimologia , Diabetes Mellitus Tipo 2/enzimologia , Estresse do Retículo Endoplasmático , Células Secretoras de Insulina/enzimologia , MAP Quinase Quinase Quinase 5/metabolismo , Sistema de Sinalização das MAP Quinases , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo , Substituição de Aminoácidos , Animais , Morte Celular/efeitos dos fármacos , Morte Celular/genética , Diabetes Mellitus Tipo 1/genética , Diabetes Mellitus Tipo 1/patologia , Diabetes Mellitus Tipo 2/genética , Diabetes Mellitus Tipo 2/patologia , Ativação Enzimática/efeitos dos fármacos , Ativação Enzimática/genética , Inibidores Enzimáticos/farmacologia , Células HEK293 , Humanos , Imidazóis/farmacologia , Insulina/genética , Insulina/metabolismo , Células Secretoras de Insulina/patologia , MAP Quinase Quinase Quinase 5/genética , Camundongos , Camundongos Knockout , Mutação de Sentido Incorreto , Piridinas/farmacologia , Proteínas Quinases p38 Ativadas por Mitógeno/antagonistas & inibidores , Proteínas Quinases p38 Ativadas por Mitógeno/genética
2.
J Biol Chem ; 282(15): 11180-7, 2007 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-17301052

RESUMO

Parkin-associated endothelin receptor-like receptor (Pael-R) is a substrate of the E3 ubiquitin ligase Parkin, which has been implicated in the pathogenesis of Parkinson disease. Misexpression of human Pael-R in Drosophila has been shown to induce selective loss of dopaminergic neurons, a symptom of Parkinson disease. Using this model, we investigated whether thioredoxin (TRX), an evolutionarily conserved antioxidant and molecular chaperone, could suppress the neurotoxicity induced by Pael-R. The Drosophila genome contains three TRX-encoding genes, namely TrxT, Trx-2, and dhd. When each of the TRX genes was overexpressed together with Pael-R in all neurons, the number of dopaminergic neurons and level of locomotor activity were significantly increased compared with control flies. To assess the role of the antioxidant activity of TRX in this context, we generated redox-defective mutants, TrxT(C35A) and TrxT(D26A/K57I), and coexpressed each of them with Pael-R. The mutants suppressed the Pael-R neurotoxicity similarly to wild-type TrxT, although the extent of the rescue was slightly reduced for the locomotor activity. We confirmed that both mutants remained active as chaperones, suggesting that this activity may be the major cause of the suppression. In the absence of Pael-R, overexpression of TRX in all neurons increased the level of locomotor activity in aged flies and extended the mean longevity by 15%. Furthermore, overexpression of TRX suppressed neurotoxicity in a Drosophila model of Machado-Joseph disease expressing polyglutamine. These results establish that Drosophila TRX can function as an anti-aging agent and as a suppressor of Pael-R- and poly-glutamine-induced neurotoxicity.


Assuntos
Proteínas de Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Longevidade/fisiologia , Neurônios/citologia , Neurônios/metabolismo , Receptores de Endotelina/metabolismo , Tiorredoxinas/metabolismo , Animais , Proliferação de Células/efeitos dos fármacos , Proteínas de Drosophila/genética , Drosophila melanogaster/citologia , Drosophila melanogaster/genética , Peróxido de Hidrogênio/farmacologia , Mutação/genética , Oxirredução , Fenótipo , Receptores de Endotelina/genética , Tiorredoxinas/genética
3.
Nihon Rinsho ; 62 Suppl 4: 24-30, 2004 Apr.
Artigo em Japonês | MEDLINE | ID: mdl-15174642
7.
Neuropathology ; 23(3): 195-8, 2003 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-14570286

RESUMO

Post-mortem examinations of the circle of Willis in two cases of subarachnoid hemorrhage disclosed a wide spectrum of vasculopathy ranging from a minimal tear between the intima and media, and between the media and adventitia, to complete transmural disruption leading to the formation of pseudoaneurysms. The presence of coexistence of the focal lesions with complete replacement of the entire arterial wall with thick fibrous connective tissues and the vasculopathy was suggestive of the spontaneous repair of recurrent non-traumatic dissection of intracranial arteries. The patients were 58-year-old and 43-year-old females. There was no history of injury to the head or neck in either case. They were hypertensive, but the degree of atherosclerotic changes in the circle of Willis was compatible with age. There was no histological evidence of vasculitis. The role of hypertension and medial mucoid degeneration in the genesis of non-traumatic dissection of intracranial arteries was discussed.


Assuntos
Dissecção Aórtica/patologia , Encéfalo/irrigação sanguínea , Encéfalo/patologia , Círculo Arterial do Cérebro/patologia , Adulto , Falso Aneurisma/etiologia , Falso Aneurisma/patologia , Feminino , Humanos , Hipertensão/complicações , Aneurisma Intracraniano/complicações , Aneurisma Intracraniano/patologia , Lúpus Eritematoso Sistêmico/complicações , Pessoa de Meia-Idade , Recidiva , Remissão Espontânea , Hemorragia Subaracnóidea/etiologia
8.
Nutr Rev ; 61(5 Pt 2): S27-33, 2003 May.
Artigo em Inglês | MEDLINE | ID: mdl-12828189

RESUMO

We examine the effects of feeding a high-sucrose diet on body weight gain, plasma triglycerides, and stress tolerance in rats. Feeding a high-sucrose (60%) diet for 2 weeks did not induce a greater body weight gain compared with that of standard diet when caloric intake was similar in ventromedial hypothalamic-lesioned obese and sham-operated lean animals. The high-sucrose diet elevated plasma triglycerides by increasing the triglyceride secretion rate and decreasing the fractional catabolic rate in both groups. In response to stress, feeding a high-sucrose diet for one week induced enhanced gene expressions of heat shock proteins (HSP 70 and 27) and suppressed NOx production in the brain, whereas the standard diet did not. Results suggest that feeding a high-sucrose diet does not induce obesity in lean rats or enhance weight gain in obese rats, if caloric intake is appropriate. The diet does elevate plasma triglyerides in lean and obese rats, but it may have the potential to improve stress tolerance.


Assuntos
Sacarose Alimentar/administração & dosagem , Estresse Fisiológico/fisiopatologia , Triglicerídeos/sangue , Aumento de Peso/efeitos dos fármacos , Animais , Ingestão de Energia , Expressão Gênica , Proteínas de Choque Térmico/genética , Humanos , Hipotálamo Médio/fisiopatologia , Óxido Nítrico Sintase/metabolismo , Obesidade/fisiopatologia
9.
Life Sci ; 72(26): 3035-46, 2003 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-12706490

RESUMO

Uncoupling proteins (UCPs) are supposed to be involved in diet-induced thermogenesis. Their activities are usually elevated by feeding and reduced by fasting in normal animals. To investigate whether fasting affects the expression of UCPs mRNA in brown adipose tissue (BAT) of bilateral ventromedial hypothalamus (VMH)-lesioned rats, we determined the gene expression of UCP1, UCP2 or UCP3 in BAT of VMH-lesioned rats and examined oxygen consumption in these rats under fed or 48-h fasted conditions. Northern blotting revealed no difference in the expression of UCPs mRNA in BAT between VMH-lesioned and sham-operated rats under the fed condition, however, expressions were increased markedly in BAT of VMH-lesioned rats under the fasted condition. Under the fed condition, no difference in oxygen consumption was observed between VMH-lesioned and sham-operated rats. Under the fasted condition, oxygen consumption decreased in both rats, however, it decreased in VMH-lesioned less than in sham operated rats. To explore the mechanism that fasting elevated BAT UCPs mRNA in VMH-lesioned rats, we measured peroxisome proliferator-activated receptor (PPAR)-gamma mRNA and protein in BAT, because PPAR-gamma agonist can elevate UCPs mRNA levels in BAT. Under the fed condition, no differences in the expression of PPAR-gamma mRNA and protein content were observed between in BAT of VMH-lesioned and sham-operated rats. Under the fasted condition, however, both increased in BAT of VMH-lesioned rats. These results suggest that VMH-lesions enhance the gene expression of UCPs in BAT under long-term fasting as a defensive reaction to inhibit the reduction of body temperature through an increase in PPAR-gamma activity.


Assuntos
Tecido Adiposo Marrom/fisiologia , Proteínas de Transporte/metabolismo , Jejum , Proteínas de Membrana Transportadoras , Proteínas Mitocondriais , Receptores Citoplasmáticos e Nucleares/metabolismo , Fatores de Transcrição/metabolismo , Desacopladores/metabolismo , Núcleo Hipotalâmico Ventromedial/metabolismo , Animais , Peso Corporal , Proteínas de Transporte/genética , Feminino , Regulação da Expressão Gênica , Canais Iônicos , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Oxigênio/metabolismo , Proteínas/genética , Proteínas/metabolismo , Ratos , Ratos Sprague-Dawley , Receptores Citoplasmáticos e Nucleares/genética , Fatores de Transcrição/genética , Proteína Desacopladora 1 , Proteína Desacopladora 2 , Proteína Desacopladora 3 , Núcleo Hipotalâmico Ventromedial/anatomia & histologia , Núcleo Hipotalâmico Ventromedial/patologia
11.
Metabolism ; 51(6): 716-23, 2002 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12037724

RESUMO

It remains unclear whether adiposity plays an important role in glucose intolerance independently of insulin resistance. We investigated whether adiposity and insulin resistance had distinct roles in glucose intolerance in rats. We examined glucose tolerance and insulin resistance using ventromedial hypothalamic (VMH)-lesioned rats in the dynamic and the static phases of obesity (2 and 14 weeks after lesioning, respectively). Rats were fed either normal chow or a fructose-enriched diet (60% of total calories). The intravenous glucose tolerance test (IVGTT) was performed by bolus injection of glucose solution (1 g/kg) and blood sampling after 0, 5 10, 30, and 60 minutes. Insulin resistance was evaluated from the steady-state plasma glucose (SSPG) value during continuous infusion of glucose, insulin, and somatostatin. SSPG was not increased in VMH-lesioned rats in the dynamic phase of obesity, but increased markedly in the static phase. The area under the glucose curve (glucose AUC) during IVGTT was increased in VMH-lesioned rats in the static phase, but not in the dynamic phase, when compared with their sham-operated counterparts. A fructose-enriched diet for 2 or 14 weeks increased SSPG values to a similar extent in both sham-operated and VMH-lesioned rats without inducing excess adiposity, but glucose intolerance was only developed in the obese rats. The plasma leptin level, an excellent indicator of adiposity, was significantly related to the glucose AUC independently of the insulin level. Insulin resistance or increased adiposity alone is not sufficient to impair glucose tolerance, but increased adiposity plays an important role in the development of glucose intolerance in an insulin-resistant state.


Assuntos
Intolerância à Glucose/fisiopatologia , Resistência à Insulina , Obesidade/fisiopatologia , Núcleo Hipotalâmico Ventromedial/fisiopatologia , Animais , Área Sob a Curva , Glicemia/efeitos dos fármacos , Peso Corporal/efeitos dos fármacos , Suplementos Nutricionais , Modelos Animais de Doenças , Ingestão de Alimentos/efeitos dos fármacos , Ácidos Graxos não Esterificados/sangue , Feminino , Frutose/administração & dosagem , Teste de Tolerância a Glucose , Insulina/sangue , Resistência à Insulina/fisiologia , Leptina/sangue , Ratos , Triglicerídeos/sangue
13.
s.l; s.n; 1992. 1 p. graf.
Não convencional em Inglês | Sec. Est. Saúde SP, HANSEN, Hanseníase, SESSP-ILSLACERVO, Sec. Est. Saúde SP | ID: biblio-1236475
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