Dataset on optimization of lignin peroxidase production by Endomelanconiopsis sp. under submerged fermentation using one factor at a time approach.

One factor at a time approach was employed for the optimization of media components, physical factors and additional additives for lignin peroxidase (LiP) production under submerged fermentation by the endophytic fungus Endomelanconiopsis sp. The optimized conditions derived were: carbon source (dextrose 10 g/L), nitrogen source (ammonium tartarate 0.027 g/L), veratryl alcohol (1 mM), MnSO4 (0.5 g/L), inoculum concentration (3 mycelial plugs), pH (5), temperature (30 °C) and Tween 80 (1.5%). A 40 fold increase of enzyme activity was observed after the single parameter optimization. The lignin peroxidase (LiP) production to the level of 345.26 ± 0.52 IU/mL indicates that the fungus has the commercial potential for LiP.

Data of optimization of laccase production by Marasmiellus palmivorus LA1 under solid state fermentation using one factor at a time method.

This article contains data regarding optimization of laccase production under solid state fermentation by the basidiomycetes fungi Marasmiellus palmivorus LA1.The data was collected by varying one factor at a time and keeping other factors of the system constant. Influence of environmental factors (substrate type, temperature, inoculums size, substrate weight, substrate length, pH), nutritional factors (organic and inorganic nitrogen sources, carbon sources, surfactants) and elicitors added to media was examined through the laccase activity of the extract. Specific activity at every stage was also measured. The data presents an opportunity to compare the relative influential role of each of the contributing factors on laccase production and directly relates to our research article "Optimization of laccase production from M. palmivorus LA1 by Taguchi method of design of experiments" (Chenthamarakshan et al., 2017 [1]).

Optimization of laccase production from Marasmiellus palmivorus LA1 by Taguchi method of Design of experiments.

BACKGROUND: Fungal laccase has profound applications in different fields of biotechnology due to its broad specificity and high redox potential. Any successful application of the enzyme requires large scale production. As laccase production is highly dependent on medium components and cultural conditions, optimization of the same is essential for efficient product production. RESULTS: Production of laccase by fungal strain Marasmiellus palmivorus LA1 under solid state fermentation was optimized by the Taguchi design of experiments (DOE) methodology. An orthogonal array (L8) was designed using Qualitek-4 software to study the interactions and relative influence of the seven selected factors by one factor at a time approach. The optimum condition formulated was temperature (28 °C), pH (5), galactose (0.8%w/v), cupric sulphate (3 mM), inoculum concentration (number of mycelial agar pieces) (6Nos.) and substrate length (0.05 m). Overall yield increase of 17.6 fold was obtained after optimization. Statistical optimization leads to the elimination of an insignificant medium component ammonium dihydrogen phosphate from the process and contributes to a 1.06 fold increase in enzyme production. A final production of 667.4 ± 13 IU/mL laccase activity paves way for the application of this strain for industrial applications. CONCLUSION: Study optimized lignin degrading laccases from Marasmiellus palmivorus LA1. This laccases can thus be used for further applications in different scales of production after analyzing the properties of the enzyme. Study also confirmed the use of taguchi method for optimizations of product production.