The Saturation- and Dose-Dependent Effects of a Teen Sexual Harassment Prevention Program: Findings from a Randomized Controlled Trial.

Using a randomized controlled trial, we investigated changes in both sexual harassment (SH) perpetration and victimization of 2104 middle school students in New York City who received divergent saturation and dosage levels of Shifting Boundaries, an SH prevention program, which was represented by the length of the program. We assessed the saturation effect of the program by comparing the outcomes across respondents from 26 schools in which there were varying percentages of students enrolled in the program. The data suggested that, overall, the program was effective in reducing sexual harassment victimization but achieved a null effect against respondents' SH perpetration and that neither the length nor the school-saturation level of the program exerted a significant effect on SH perpetration. Although the data indicated a significant difference in SH victimization between the treatment and control group, when comparing subgroups who received treatment with divergent saturation and dosage levels, no statistically significant difference was identified. Our results suggested that the program effect was not contingent on the portion of students in a school who enrolled in the program, nor was it contingent on the dosage.

Fracturas humerales por fragilidad en un hospital de tercel nivel. Características clínicas y epidemiológicas / Humeral fragility fractures in a tertiary referral hospital. Clinical and epidemiological characteristics

Objetive: Humeral fragility fractures represent an important complication of osteoporosis as they rank the third most prevalent in individuals over sixty years old. Our study aims to analyze the prevalence and treatment of the humeral fragility fractures in a tertiary referral hospital. Material and methods: Retrospective study of those patients presenting humeral fragility fractures who attended a tertiary referral hospital during 2013. Clinical and epidemiological variables were collected, and the incidence of new fractures and that of mortality was analyzed over a three-year period. Results: 248 humeral fragility fractures were analyzed. 81% of the patients were women whose average age was 71 years. 28.2% of the patients have suffered a previous fracture and 20.2% of them suffered one at a later time. 12.5% had been previously diagnosed with osteoporosis and only 9.2% got this bone metabolic condition diagnosed after fracturing the humerus. 18% of patients passed away during the follow-up period. Conclusions: In our area of expertise, humeral fragility fractures are followed by a low percentage of underlying osteoporosis cases being diagnosed and treated, what may be the trigger to a rise in the risk of new fractures. (AU)

Objetivo: Las fracturas humerales por fragilidad representan una importante complicación de la osteoporosis, llegando a ser las terceras más prevalentes en los individuos mayores de 60 años. El objetivo de este trabajo ha sido analizar la prevalencia y manejo de las fracturas humerales por fragilidad en un hospital de tercer nivel. Material y métodos: Estudio retrospectivo de los pacientes con fractura humeral por fragilidad atendidos en un hospital terciario universitario durante el año 2013. Se recogieron las variables clínico-epidemiológicas y se analizó la incidencia de nuevas fracturas y la mortalidad en un periodo de seguimiento de 3 años. Resultados: Se analizaron 248 fracturas humerales por fragilidad. El 81% de los pacientes eran mujeres y la media de la edad fue de 71 años. El 28,2% de los pacientes había presentado una fractura previa y el 20,2% sufrió una posterior. El 12,5% había sido diagnosticado previamente de osteoporosis, y solamente el 9,2% recibió el diagnóstico de este trastorno metabólico óseo tras la fractura humeral. Un 18% de los pacientes fallecieron durante el periodo de seguimiento. Conclusiones: La fractura humeral por fragilidad se sigue, en nuestro medio, de un bajo porcentaje de diagnóstico y tratamiento de la osteoporosis subyacente, lo que puede condicionar un aumento del riesgo de nuevas fracturas. (AU)

Quantification of classical HLA class I mRNA by allele-specific, real-time polymerase chain reaction for most Han individuals.

Recent studies have shown that expression levels of different alleles at the same HLA class I locus can vary dramatically, which might have a broad influence on human disease. However, precise quantification of the relative expression level of each HLA allele is challenging, because distinguishing different alleles on the same locus is difficult. Here, we developed a series of allele-specific, real-time polymerase chain reaction assays for quantifying HLA class I allele mRNA in most Han individuals. The alleles of almost all heterozygous genotypes with a frequency higher than 0.5% in our population (78 alleles on HLA-A locus, 124 alleles on HLA-B locus, and 74 alleles on HLA-C locus) were specifically amplified. The specificity of the amplification was strictly validated by setting the corresponding negative control for each allele of each genotype. The amplification efficiency of each reaction was determined, and the slopes of the reactions were compared. This study provides a tool for detecting the comprehensive expression profile of HLA class I alleles and will be useful not only for the investigation of the molecular mechanism underlying HLA allele expression regulation but also for exploration of immunological mechanisms involving HLA expression in the fields of tumour immune evasion, viral infection, auto-immune disorders, and graft vs host disease after haematopoietic stem cell transplantation.

Microarray based analysis of gene expression patterns in pancreatic neuroendocrine tumors.

OBJECTIVE: Pancreatic neuroendocrine tumors (PanNETs) are a small subgroup of tumors with a variety of biological behaviors. MATERIALS AND METHODS: We sought to identify the specially expressed genes and characterize significant pathways in PanNETs compared with non-neoplastic samples. Gene expression profile GSE43795 was obtained from Gene Expression Omnibus database, which included 6 PanNETs and 5 non-neoplastic samples. The differentially expressed genes (DEGs) were identified using Limma package. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were used to enrich the functions and pathways of DEGs. Transcription factors (TFs) and tumor-associated genes (TAGs) were also identified. Finally, a protein-protein interaction (PPI) network was constructed, and hub proteins and functional module were screened out. RESULTS: Total of 821 DEGs (421 down-regulated, 400 up-regulated) were selected. GO and KEGG enrichment analyses showed that up-regulated DEGs were related to several pathways, including type 2 diabetes mellitus, Ca2+ signaling pathway, long-term potentiation, and long-term depression pathways. Down-regulated DEGs were enriched in several pathways, such as pancreatic secretion, protein digestion and absorption, and metabolic pathway. Interferon-stimulated gene protein 15 (ISG15), somatostatin (SST), and synaptosomal-associated protein 25 kDa (SNAP25) were identified as hub proteins. CONCLUSIONS: The genes involved in type 2 diabetes mellitus pathway may play important roles in the development of PanNETs. SNAP25, SST, and ISG15 may be used as potential targets for treatment of PanNETs.

Modeling system for predicting enterococci levels at Holly Beach.

This paper presents a new modeling system for nowcasting and forecasting enterococci levels in coastal recreation waters at any time during the day. The modeling system consists of (1) an artificial neural network (ANN) model for predicting the enterococci level at sunrise time, (2) a clear-sky solar radiation and turbidity correction to the ANN model, (3) remote sensing algorithms for turbidity, and (4) nowcasting/forecasting data. The first three components are also unique features of the new modeling system. While the component (1) is useful to beach monitoring programs requiring enterococci levels in early morning, the component (2) in combination with the component (1) makes it possible to predict the bacterial level in beach waters at any time during the day if the data from the components (3) and (4) are available. Therefore, predictions from the component (2) are of primary interest to beachgoers. The modeling system was developed using three years of swimming season data and validated using additional four years of independent data. Testing results showed that (1) the sunrise-time model correctly reproduced 82.63% of the advisories issued in seven years with a false positive rate of 2.65% and a false negative rate of 14.72%, and (2) the new modeling system was capable of predicting the temporal variability in enterococci levels in beach waters, ranging from hourly changes to daily cycles. The results demonstrate the efficacy of the new modeling system in predicting enterococci levels in coastal beach waters. Applications of the modeling system will improve the management of recreational beaches and protection of public health.

Serum uric acid is associated with quantitative ultrasound parameters in men: data from the Camargo cohort.

UNLABELLED: This study analyzes the association between serum uric acid levels and heel quantitative ultrasound (QUS) parameters in men aged 50 or more from the Camargo cohort. We found that higher serum uric acid levels are positively associated with all QUS measurements, suggesting a better bone quality in men with elevated serum uric acid values. INTRODUCTION: Higher serum uric acid concentrations have been associated with higher bone mineral density and lower prevalence of fractures. However, there are no studies that have assessed the bone quality properties in Caucasians. Therefore, we have analyzed the association between quantitative ultrasound (QUS) and serum uric acid levels in adult men from a population-based cohort. METHODS: A total of 868 men older than 50 were recruited from a larger cohort (Camargo Cohort) after excluding those with any known condition or drug treatment with a possible influence on bone metabolism, or those with a previous diagnosis of gout or taking hipouricemic agents. Bone turnover markers (PINP and CTX), 25OH-vitamin D and PTH levels were measured by electrochemiluminiscence. BMD was determined by DXA, and heel QUS with a gel-coupled device. RESULTS: Lumbar, femoral neck and total hip BMD was significantly higher in men with higher serum uric acid levels. QUS parameters were also significantly higher in men with high uric acid levels than those with lower values, and increased continuously across quartiles after adjustment for confounding variables. In multiple regression analysis, serum uric acid was significantly associated with all QUS parameters. Finally, men with serum acid levels above median showed higher values in all the QUS parameters than men with lower values. CONCLUSIONS: Higher serum uric acid levels in men older than 50 years are positively associated with QUS parameters. These data might suggest a better bone quality in men with elevated serum uric acid values.

Effectiveness of "shifting boundaries" teen dating violence prevention program for subgroups of middle school students.

PURPOSE: We examine whether the Shifting Boundaries (SB) intervention, a primary intervention to prevent youth dating violence and sexual harassment (DV/H), is differentially effective for girls compared with boys or for youth with a history of DV/H experiences. METHODS: We randomly assigned SB to 30 public middle schools in New York City, enrolling 117 sixth and seventh grade classes to receive a classroom, building, combined, or neither intervention. The SB classroom intervention included six sessions emphasizing the laws/consequences of DV/H, establishing boundaries and safe relationships. The SB schoolwide/building intervention included the use of school-based restraining orders, greater faculty/security presence in unsafe "hot spots" mapped by students, and posters to increase DV/H awareness and reporting. Student surveys were implemented at baseline, immediately after intervention, and 6 months after intervention. RESULTS: At 6 months after intervention, the SB building-level intervention was associated with significant reductions in the frequency of sexual harassment (SH) perpetration and victimization; the prevalence and frequency of sexual dating violence victimization; and the frequency of total dating violence victimization and perpetration. We also had one anomalous finding that the interventions were associated with an increase in the prevalence of SH victimization. These results were consistent for girls and boys, and those with or without a history of DV/H, with the one exception for those exposed to the SB building condition who had earlier reported perpetrating SH had a significantly lower frequency of perpetrating SH at the follow-up than those without such a history. CONCLUSIONS: SB can provide effective universal prevention of middle school DV/H experiences, regardless of students' prior exposure histories, and for boys and girls.

Shifting Boundaries: an experimental evaluation of a dating violence prevention program in middle schools.

We randomly assigned the Shifting Boundaries interventions to 30 public middle schools in New York City, enrolling 117 sixth and seventh grade classes (over 2,500 students) to receive a classroom, a building, a combined, or neither intervention. The classroom intervention included a six-session curriculum emphasizing the laws and consequences for perpetrators of dating violence and sexual harassment (DV/H), the social construction of gender roles, and healthy relationships. The building-based intervention included the use of building-based restraining orders, higher levels of faculty/security presence in safe/unsafe "hot spots" mapped by students, and posters to increase DV/H awareness and reporting. Student surveys were implemented at baseline, immediately after the intervention, and 6-months post-intervention. As hypothesized, behaviors improved as a result of the interventions. The building-only and the combined interventions were effective in reducing sexual violence victimization involving either peers or dating partners at 6-months post-intervention. This was mirrored by reductions in sexual violence perpetration by peers in the building-only intervention. While the preponderance of results indicates that the interventions were effective, an anomalous result (increase in sexual harassment victimization reports that was contradicted by lower frequency estimates) did emerge. However, after analysis these anomalous results were deemed to be most likely spurious. The success of the building-only intervention alone is important because it can be implemented with very few extra costs to schools.

Surgical site infections in cardiac surgery after a hospital catastrophe.

On 2 November 1999, one of the main hospital façades adjoining cardiovascular surgery collapsed in a 900-bed teaching hospital in Santander, Spain. The purpose of this study was to determine whether the accident affected the safety of patients by increasing the risk for nosocomial and surgical site infections (SSI). Measures for the prevention of nosocomial infections were immediately reinforced. A total of 217 consecutive patients were operated on before 2 November 1999, with another 296 after this date. Patients in both study periods showed similar severity of illness, complexity of surgical procedure and length of hospital stay. The overall rate of nosocomial infection before and after the accident was 28.1% and 24.7%, respectively (P=0.381). The rates of respiratory infection, urinary infection and bacteraemia were also similar. A statistically significant reduction in the SSI rate in the second period was observed (14.8% vs 4.4%, P=0.008). The collapse of the façade was not associated with any increase in nosocomial infection rates, but there was a significant reduction of SSI rates in relation to intensive infection control measures implemented after the collapse.

Progressive cardiomyopathy as manifestation of mitochondrial disease.

Cardiomyopathies are a clinically and genetically heterogeneous group of cardiac diseases in which the myocardium is primarily involved. Mitochondrial DNA point mutations have been identified in a broad spectrum of mitochondrial disorders, which are associated with neurological diseases. However, they also have been reported in patients with cardiomyopathy, either alone or as part of a multisystem disorder. A patient who presented with severe heart failure and was diagnosed as having a mitochondrial A3243G mutation is described.

[Combined construction of rainwater catchment techniques with methane pool and greenhouse].

A combined structure of rainwater catchment, methane pool and reformed greenhouse was constructed to improve water use efficiency. The results showed that the combination of rainwater catchment, reserving techniques and water saving measures was efficient to resolve the problem of water deficiency in the semi-arid region with precipitation of 250-550 mm. The combined structure of water catchment engineering, greenhouse and methane pool redistributed light, temperature, water and heat to develop higher benefits economic crops, and improved water use efficiency, which made it possible to develop high yield, and best quality agriculture in this region. Through optimizing the ecological factor in greenhouse, the water use was reduced by 50%-70%, and the soil temperature and night temperature were raised. This combined construction also improved productivity in greenhouse, and efficiently controlled plant diseases and insect pests. Methane pool provided CO2 and part energy source. The shady area back of the greenhouse was exploited by growing shady crops and epiphyte, which contributed to exchange energy and CO2 between light and shady house. This model could be used as water catchment agricultural development model in the region, and some other new approaches to water catchment agriculture in semi-arid region were discussed in this paper.

Insuficiencia renal por sulfadiacina en pacientes con encefalitis por toxoplasma / Acute reanl failure following the use of sulfadiazine in patients with toxoplasma encephalitis

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