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1.
Animal ; 17(5): 100804, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-37141635

RESUMO

Over the years, there has been considerable variation in the bull conception rate (BCR) of Japanese Black cattle; moreover, several Japanese Black bulls with a low BCR of ≤10% have been identified. However, the alleles responsible for the low BCR are not determined yet. Therefore, in this study, we aimed to identify single-nucleotide polymorphisms (SNPs) for predicting low BCR. To this end, the genome of Japanese Black bulls was comprehensively examined by a genome-wide association study with whole-exome sequencing (WES), and the effect of the identified marker regions on BCR was determined. The WES analysis of six sub-fertile bulls with a BCR of ≤10% and 73 normal bulls with a BCR of ≥40% identified a homozygous genotype for low BCR in Bos taurus autosome 5 in the region between 116.2 and 117.9 Mb. The g.116408653G > A SNP in this region had the most significant effect on the BCR (P-value = 1.0 × 10-23), and the GG (55.4 ± 11.2%) and AG (54.4 ± 9.4%) genotypes in the SNP had a higher phenotype than the AA (9.5 ± 6.1%) genotype for the BCR. The mixed model analysis revealed that g.116408653G > A was related to approximately 43% of the total genetic variance. In conclusion, the AA genotype of g.116408653G > A is a useful index for identifying sub-fertile Japanese Black bulls. Some positive and negative effects of SNP on the BCR were presumed to identify the causative mutations, which can help evaluate bull fertility.


Assuntos
Fertilização , Estudo de Associação Genômica Ampla , Bovinos/genética , Animais , Masculino , Estudo de Associação Genômica Ampla/veterinária , Alelos , Fertilização/genética , Genótipo , Fertilidade/genética , Polimorfismo de Nucleotídeo Único
2.
J Neuroendocrinol ; 27(1): 57-65, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25367275

RESUMO

A luteinising hormone (LH) surge is fundamental to the induction of ovulation in mammalian females. The administration of a preovulatory level of oestrogen evokes an LH surge in ovariectomised females, whereas the response to oestrogen in castrated males differs among species; namely, the LH surge-generating system is sexually differentiated in some species (e.g. rodents and sheep) but not in others (e.g. primates). In the present study, we aimed to determine whether there is a functional LH surge-generating system in male goats, and whether hypothalamic kisspeptin neurones in male goats are involved in the regulation of surge-like LH secretion. By i.v. infusion of oestradiol (E2; 6 µg/h) for 16 h, a surge-like LH increase occurred in both castrated male and ovariectomised female goats, although the mean peak LH concentration was lower and the mean peak of the LH surge was later in males compared to females. Dual staining with KISS1 in situ hybridisation and c-Fos immunohistochemistry revealed that E2 treatment significantly increased c-Fos expression in the medial preoptic area (mPOA) KISS1 cells in castrated males, as well as ovariectomised females. By contrast, dual-labelled cells were scarcely detected in the arcuate nucleus (ARC) after E2 treatment in both sexes. These data suggest that kisspeptin neurones in the mPOA, but not those in the ARC, are involved in the induction of surge-like LH secretion in both male and female goats. In summary, our data show that the mechanism that initiates the LH surge in response to oestrogen, the mPOA kisspeptin neurones, is functional in male goats. Thus, sexual differentiation of the LH surge-generating system would not be applicable to goats.


Assuntos
Kisspeptinas/metabolismo , Hormônio Luteinizante/biossíntese , Neurônios/metabolismo , Área Pré-Óptica/metabolismo , Animais , Feminino , Cabras , Hibridização In Situ , Kisspeptinas/genética , Hormônio Luteinizante/sangue , Masculino , Área Pré-Óptica/citologia
3.
J Neuroendocrinol ; 24(9): 1234-42, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22536815

RESUMO

Follicular development and ovulation are strongly suppressed during lactation in mammals via a profound suppression of gonadotrophin secretion. The present study aimed to examine the role of oestrogen feedback action in suppressing luteinising hormone (LH) secretion and hypothalamic kisspeptin expression during the latter half of lactation. Plasma LH concentrations kept at low levels throughout the lactating period in intact and oestrogen-replaced ovariectomised (OVX) lactating rats, whereas plasma LH concentrations gradually elevated from day 10 postpartum in lactating OVX rats. OVX lactating rats showed frequent LH pulses at late lactation, although the LH pulses were significantly inhibited by an oestrogen replacement, which is much less effective on LH release in nonlactating rats. Oestrogen replacement in lactating OVX rats significantly reduced the number of Kiss1 mRNA-expressing cells in the arcuate nucleus (ARC) at late lactation, although the same oestrogen treatment did not affect the number of Kiss1-expressing cells in nonlactating controls. Exogenous kisspeptin challenge (0.2 nmol) into the third cerebroventricle significantly increased LH secretion in lactating OVX, lactating OVX + subcutaneous 17ß-oestradiol and intact lactating rats at day 16 postpartum. These results suggest that LH pulse suppression during late lactation could be a result of the enhanced oestrogen-dependent suppression of ARC kisspeptin expression.


Assuntos
Núcleo Arqueado do Hipotálamo/metabolismo , Estradiol/fisiologia , Kisspeptinas/biossíntese , Lactação/metabolismo , Lactação/fisiologia , Hormônio Luteinizante/metabolismo , Animais , Núcleo Arqueado do Hipotálamo/efeitos dos fármacos , Estradiol/sangue , Estradiol/farmacologia , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica/fisiologia , Injeções Intraventriculares , Kisspeptinas/administração & dosagem , Kisspeptinas/farmacologia , Hormônio Luteinizante/sangue , Núcleos da Linha Média do Tálamo/efeitos dos fármacos , Núcleos da Linha Média do Tálamo/metabolismo , Ovariectomia/métodos , Ratos , Ratos Wistar , Fatores de Tempo
4.
Horumon To Rinsho ; 18(4): 339-44, 1970 Apr.
Artigo em Japonês | MEDLINE | ID: mdl-5464727
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