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1.
J Med Eng Technol ; 35(2): 103-8, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-21204610

RESUMO

Breast cancer is one of the most important diseases in females worldwide. According to the Malaysian Oncological Society, about 4% of women who are 40 years old and above are involved have breast cancer. Masses and microcalcifications are two important signs of breast cancer diagnosis on mammography. Enhancement techniques, i.e. histogram equalization, histogram stretching and median filters, were used to provide better visualization for radiologists in order to help early detection of breast abnormalities. In this research 60 digital mammogram images which includes 20 normal and 40 confirmed diagnosed cancerous cases were selected and manipulated using the mentioned techniques. The original and manipulated images were scored by three expert radiologists. Results showed that the selected methods have a positive significant effect on image quality.


Assuntos
Mamografia/métodos , Feminino , Humanos , Intensificação de Imagem Radiográfica
2.
J Biochem Mol Biol Biophys ; 6(5): 301-8, 2002 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-12385964

RESUMO

Four ADP-glucose pyrophosphorylase cDNA clones were isolated from mature leaves and pith of sago palm by the polymerase chain reaction (PCR) technique. Three of them (agpp10, agpp12 and agpl19) encoded the AGP large subunit, while the fourth clone (agpl1) encoded the small subunit. agpp10 and agpp12 were isolated from pith, agpl19 was isolated from mature leaves, while agpl1 from both tissues. In addition, a full-length cDNA of agpl1 was successfully isolated from a cDNA library of mature leaves by a PCR-based screening technique. Semi-quantitative analysis suggests that agpp10 and agpp12 were detectable only in pith, agpl19 only in leaves, while agpl1 was expressed in both leaves and pith tissues.


Assuntos
Arecaceae/genética , DNA Complementar/genética , Nucleotidiltransferases/genética , Sequência de Aminoácidos , Arecaceae/enzimologia , Sequência de Bases , Clonagem Molecular , Sequência Consenso , Primers do DNA , DNA Complementar/isolamento & purificação , Glucose-1-Fosfato Adenililtransferase , Dados de Sequência Molecular , Nucleotidiltransferases/química , Plantas/enzimologia , Plantas/genética , Reação em Cadeia da Polimerase/métodos , Subunidades Proteicas , Proteínas Recombinantes/química , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos
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