Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 4 de 4
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Bull Exp Biol Med ; 169(1): 77-80, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32488785

RESUMO

An association was found between reduced expression of miR-34a, miR-146a with both metastasis to regional lymph nodes (relative risk RR=10.50 and RR=5.25, respectively) and the development of distant metastases (RR=9.50 and RR=4, 40, respectively) in gastric cancer. They are excellent classifiers: AUC>0.9 for both miRNAs. The association of miR-335 expression with metastasis to the lymph nodes is much weaker, but it is also a good classifier for identifying a group with distant metastasis (RR=5.90). A correlation was found between the expression of miR-34a and miR-146a during metastasis, which is absent in non-metastatic tumors. Thus, miR-34a, miR-146a, and miR-335 miRNAs can be proposed as candidates for biomarkers of the risk of gastric cancer metastasis.


Assuntos
Adenocarcinoma/diagnóstico , Biomarcadores Tumorais/genética , MicroRNAs/genética , Neoplasias Gástricas/diagnóstico , Adenocarcinoma/genética , Adenocarcinoma/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Metástase Neoplásica , Prognóstico , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia , Transcriptoma
2.
Ann Oncol ; 21(9): 1779-1785, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20150573

RESUMO

BACKGROUND: This retrospective study was carried out to evaluate the prognostic significance of clinical factors in patients treated for metastatic gastric cancer with second-line chemotherapy. PATIENTS AND METHODS: We evaluated the prognostic significance of various clinical factors in 126 patients, who were treated with second-line chemotherapy. RESULTS: Median progression-free and overall survival (OS) for second-line chemotherapy were 3.3 and 5.3 months, respectively, with an overall response rate of 11.1%. Multivariate analysis identified three independent prognostic factors: performance status: Eastern Cooperative Oncology Group zero to one [hazard ratio (HR) 2.3, 95% confidence interval (CI) 1.7-5.4], hemoglobin (Hb) level: >/=10 g/dl (HR 2.2, 95% CI 2.1-2.4) and time-to-progression (TTP) under first-line therapy: >/=5 months (HR 0.5, 95% CI 0.3-0.8). From the obtained data, a prognostic index was constructed, dividing the patients into three risk groups: good (n = 40), intermediate (n = 36) and poor risk group (n = 56). The median survival for good, intermediate and poor risk groups were 13.5, 6.0 and 2.9 months, respectively, whereas the 1-year OS rates were 50.2%, 14.2% and 2.6%, respectively (P = 0.00001). CONCLUSIONS: With inadequate data from randomized controlled trials at the moment, our report indicates that second-line chemotherapy is effective and beneficial in patients with good performance status, higher Hb level along with higher TTP under first-line therapy.


Assuntos
Adenocarcinoma/tratamento farmacológico , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Carcinoma de Células em Anel de Sinete/tratamento farmacológico , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Ovarianas/tratamento farmacológico , Neoplasias Peritoneais/tratamento farmacológico , Neoplasias Gástricas/tratamento farmacológico , Adenocarcinoma/patologia , Adulto , Idoso , Carcinoma de Células em Anel de Sinete/patologia , Cisplatino/administração & dosagem , Docetaxel , Etoposídeo/administração & dosagem , Feminino , Fluoruracila/administração & dosagem , Seguimentos , Humanos , Neoplasias Hepáticas/secundário , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Mitomicina/administração & dosagem , Estadiamento de Neoplasias , Neoplasias Ovarianas/secundário , Neoplasias Peritoneais/secundário , Prognóstico , Estudos Retrospectivos , Neoplasias Gástricas/patologia , Taxa de Sobrevida , Taxoides/administração & dosagem
3.
Clin Chem ; 46(8 Pt 1): 1078-84, 2000 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10926886

RESUMO

BACKGROUND: Cell-free DNA from dying cells recently has been discovered in human blood plasma. In experiments performed on animals and humans, we examined whether this cell-free DNA can cross the kidney barrier and be used as a diagnostic tool. METHODS: Mice received subcutaneous injections of either human Raji cells or purified (32)P-labeled DNA. DNA was isolated from urine and analyzed by measurement of radioactivity, agarose gel electrophoresis, and PCR. In humans, the permeability of the kidney barrier to polymeric DNA was assessed by detection in urine of sequences that were different from an organism bulk nuclear DNA. RESULTS: In the experiments on laboratory animals, we found that approximately 0.06% of injected DNA was excreted into urine within 3 days in a polymeric form and that human-specific ALU: sequences that passed through the kidneys could be amplified by PCR. In humans, male-specific sequences could be detected in the urine of females who had been transfused with male blood as well as in DNA isolated from urine of women pregnant with male fetuses. K-ras mutations were detected in the urine of patients with colon adenocarcinomas and pancreatic carcinomas. CONCLUSIONS: The data suggest that the kidney barrier in rodents and humans is permeable to DNA molecules large enough to be analyzed by standard genetic methodologies.


Assuntos
DNA/urina , Animais , Transfusão de Sangue , Morte Celular , Neoplasias Colorretais/química , Neoplasias Colorretais/patologia , Neoplasias Colorretais/urina , DNA/genética , Eletroforese em Gel de Ágar , Feminino , Feto/química , Genes ras , Genoma , Humanos , Rim/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C3H , Mutação , Neoplasias Pancreáticas/química , Neoplasias Pancreáticas/urina , Reação em Cadeia da Polimerase , Gravidez , Células Tumorais Cultivadas , Cromossomo Y
4.
Ter Arkh ; 61(3): 34-7, 1989.
Artigo em Russo | MEDLINE | ID: mdl-2741114

RESUMO

Examination of 155 patients with atopic, infection-dependent bronchial asthma (BA) and chronic asthmatic bronchitis in the phase of the disease exacerbation and remission has shown that hyperhistaminemia is common not only to atopic but also to infection-dependent BA. It is established that activation of lipid peroxidation of the membranes more pronounced during exacerbation and attacks of asthma is one of the most important alternative mechanisms of mast cell activation, that is supported by the data of experimental morphologic and biochemical studies of the effect exerted by peroxidized fatty acids on histamine liberation and mast cell degranulation.


Assuntos
Asma/metabolismo , Peroxidação de Lipídeos , Mastócitos/fisiologia , Adulto , Animais , Asma/etiologia , Asma/fisiopatologia , Bronquite/sangue , Membrana Celular/metabolismo , Histamina/sangue , Humanos , Infecções/complicações , Pessoa de Meia-Idade , Ratos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...