Manganese Defective Clustering: Influence on the Spectroscopic Features of Ceria-Based Nanomaterials.

The influence of manganese modification on the spectroscopic features of manganese-doped CeO2 systems synthesized by the microwave-assisted hydrothermal route and their correlation with the presence of O defective structures were verified, focusing on their interaction with poisonous atmospheres. Raman and electron paramagnetic resonance studies confirmed the presence of defective clusters formed by dipoles and/or quadrupoles. The number of paramagnetic species was found to be inversely proportional to the doping concentration, resulting in an increase in the Mn2+ signal, likely due to the reduction of Mn3+ species after the interaction with CO. X-ray photoelectron spectroscopy data showed the pure system with 33% of its cerium species in the Ce3+ configuration, with an abrupt decrease to 19%, after the first modification with Mn, suggesting that 14% of the Ce3+ species are donating one electron to the Mn2+ ions, thus becoming nonparamagnetic Ce4+ species. On the contrary, 58% of the manganese species remain in the Mn2+ configuration with five unpaired electrons, corroborating the paramagnetic feature of the samples seen in the electron paramagnetic resonance study.

Salivary biomarkers present in patients with periodontitis without clinical distinction: findings from a meta-analysis.

BACKGROUND: A new classification for periodontitis has been adopted in clinical practice. However, there are still discussions regarding this new classification and difficulties in its adoption, both by professionals and researchers. Thus, this study aimed to evaluate which salivary biomarkers are present in periodontitis, following the new classification of periodontal diseases through meta-analysis. MATERIAL AND METHODS: A literature search was carried out in the scientific databases: PubMed, Scielo and Google scholar to select studies. The selection of studies was followed by two authors upon reading of the title, abstract and full text. The necessary data were collected and statistical analyses were performed using the Review Manager statistical software version 5.4, with calculation of Mean Difference, heterogeneity (I²) and funnel plot with P < 0.05. RESULTS: After following the selection criteria, 9 articles were selected for comparison. The studies address the presence of biomarkers in the saliva of patients with periodontitis and their possible use in the monitoring and diagnosis of the disease. For the meta-analytic comparison, a sample size of 1,983 individuals was used. Statistical analyses showed that nitric oxide, IL-6, IL-1B and osteoprotegerin are substances that are significantly present in patients with periodontitis (P < 0.05). CONCLUSIONS: IL-6, nitric oxide, IL-1B, TNF-α and osteoprotegerin are among the most present biomarkers in patients with periodontitis, and may be used in the future as a monitoring of periodontal disease. The present study also revealed that there was no statistically significant difference in the concentration of these biomarkers for clinical distinction from periodontitis.

Critical study of lactic dehydrogenase isoenzymes by electrophoresis in the diagnosis of cardiac patients.

Com o objetivo de estudar os padroes de perfis das isoenzimas do LD por fracionamento eletroforetico, os autores relacionaram um grupo normal controle e um grupo de pacientes na fase aguda do infarto do miocardio e com sindrome intermediaria.Para cada patologia foram determinados os padroes de liberacao do LD e HBD a fim de correlaciona-los com a curva de liberacao enzimatica do LD1. A inversao do LD (LD1 > LD2) foi estudada no seu "real significado" com dado diagnostico laboratorial, quando correlacionada a interpretacao clinica (foram usados valores referencia, segundo Galen, para este proposito). Seu valor preditivo pode ser de grande valia para o clinico, porem, o uso deste dado isoladamente pode levar a conclusoes erroneas em pacientes infartados agudos e com sindrome intermediaria. A correlacao entre LD 1 e o HBD foi positiva e se discute sua aplicabilidade sao calculados com base em valores referencia tanto para pacientes na fase aguda do infarto do miocardio, quanto da sindrome intermediaria

Evaluation of electrophoretic and immunological inhibition methods for the determination of creatine kinase isoenzyme activity.

Os autores compararam 2 "kits" para a determinacao da atividade do CKMB: A - eletroforese (Helena do Brasil) e B - inibicao imunologica (CKMB - test UV Merck I test) para selecionar um dos dois para uso A e B foram comparados em grupos de normais e pacientes infartados, quanto a: 1.Exatidao - A: controle do CKMB corrido em toda serie de fitas; B: calibracao do metodo com soro controle Precinorm E. 2.Precisao - coeficiente de variacao (C.V.): A: 6,7% e B: manual: 3,1% e automatico: 0,9%.3. Especificidade - A 58%; B: 100%. 4.Sensibilidade - A: 71%; B: 100%. 5. Eficiencia diagnostica - A: 60,7%; B: 100%. 6.Indice de Youden - A: 0,29; B: 1,0. 7. Preditividade - A: 58%; B: 100%. Outras caracteristicas, tais como: 8. Tempo de realizacao - A: 1h 30 min; B: 10 min. 9. Equipamento - B mais simples. 10. Experiencia do tecnico - B menor. 11. Custo - B menor. A partir destes dados e pelo fato de ser a dosagem do CKMB um exame de urgencia os autores recomendam utilizar o metodo da inibicao imune

A importancia da determinacao do valor da hemoglobina no calculo do excesso de base (BE) em um hospital de grande porte. / Importance of the determination of hemoglobin levels for the calculation of base excess parameters in a general hospital

No presente trabalho, os autores chamam a atencao para a importancia do calculo de cada Excesso de Base (BE) baseado no valor da respectiva concentracao de hemoglobina (Hb), ja que encontraram 87% de niveis de hemoglobina abaixo de 15,0 g/dl em sua casuistica. Foi estudada uma centena de casos e foram comapardos os valores em 2 grupos: I) o valor real da hemoglobina do pacientes e II) considerando a hemoglobina como 15,0 g/dl. Foi usado o teste de McNemar para tal comparacao e se observou uma discordancia significativa (X2 = 17,47).Outro nivel de igualdade foi medido por um teste t pareado para a concentracao da Hb em 2 series de amostras sanguineas dos mesmos pacientes, para comparar EDTA e heparina como anticoagulantes. Nao se encontrou nenhuma diferenca significativa. O uso de heparina somente como anticoagulante tornou a colheita mais simples, por ser usada para ambos os testes (Hb e BE)