RESUMO
The chemical composition and structural characterization of exopolysaccharides from the fungus Lasiodiplodia theobromae MMBJ are described, and the immunomodulatory activity of a purified ß-glucan was evaluated. L. theobromae MMBJ produced three different ß-glucans. One, fraction PEPS, was a branched (1â3)(1â6)-ß-glucan and was insoluble in cold water. The other two, fractions SEPS-005R and SEPS-10E, were characterized as linear (1â6)-ß-glucans with molar mass of 1.8×10(6)Da and 7.0×10(3)Da, respectively. From a total of 2.2g/L of EPS produced by L. theobromae through submerged fermentation, 1.5g/L (67%) was of the branched (1â3)(1â6)-ß-glucan, while 25% (w/w) were linear (1â6)-ß-glucans. Tests conducted with macrophages showed that the high molar mass (1â6)-ß-glucan fraction (SEPS-005R) induced a pro-inflammatory response pattern.