Individual and combinative effect of NaCl and γ-radiation on NADPH-generating enzymes activity in corn (Zea mays L.) sprouts.

Being a universal reducing agent nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) plays an important role in the cellular metabolism and the implementation of anti-stress reactions in plants. There are only a few enzymes that ensure the NADPH pool formation in cells. Among them, the most important are glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49), malate dehydrogenase decarboxylating (DMDH, malic enzyme, EC 1.1.1.40) and NADP-isocitrate dehydrogenase (NADP-IDH, EC 1.1.1.42). The presented investigation is devoted to studying the influence of the individual and combinative effects of NaCl and γ-radiation as abiotic stress factors on biometric indicators and activity of these NADPH-generating enzymes, on organic content, and the formation of paramagnetic centers as defense reaction in corn (Zagatala-68 genotype) sprouts. It was found that 100 mM NaCl had an inhibitory effect on the development of sprouts. Relatively lower doses (50 Gy and 100 Gy) of γ-radiation had a positive, but its higher doses (150 Gy and 200 Gy) had a negative effect on this process. 500 Gy was a lethal dose (LD) for the corn sprouts. Combinative stress in all cases considerably delayed the development of sprouts. G6PDH showed the highest activity in the first, whereas, NADP-IDH showed the same activity in the last days of the experiment. All three enzymes, especially the G6PDH, have been activated in both root and stem tissues under the influence of stress factors (either radiation or salt). Combinative stress (γ-radiation + salt) also led to an induction of these activities which was necessary to neutralize the negative consequences of stress factors. Stress factors in all cases also had a negative effect on the content of organic matter in seedlings. Ionizing gamma radiation, which resulted in the formation of new paramagnetic centers as an anti-stress defense reaction in many cases was observed in wheat seedlings, but not in corn sprouts, which clearly shows that there are some differences in the protective mechanisms of these C3- and C4-types of plants to γ-radiation.

The synergistic effects of betanin and radiotherapy in a prostate cancer cell line: an in vitro study.

BACKGROUND: Prostate cancer is among the most common cancers in men with an increasing incidence rate. Radiation therapy (RT) is a therapeutic strategy for the management of prostate cancer after surgery; nonetheless, it has different side effects on neighboring healthy cells/tissues. Moreover, radioresistance has been an increasing phenomenon in the recent years. Therefore, there is an urgent need for the introduction of a safe and effective radiosensitizing agent. Accordingly, the recent trend in the development of novel drugs is accompanied by a push toward natural compounds. Our study evaluated the effects of betanin combined with RT as a potential radiosensitizing agent in the PC-3 cell line. METHODS AND RESULTS: MTT assay was utilized to determine the growth inhibitory impact of betanin. The possible synergistic effect was evaluated with CompuSyn software upon Trypan blue exclusion test. Apoptosis-related gene expression was evaluated via Real-time PCR and the protein expression of P21 was determined using western blotting. A synergistic anticancer effect with an optimal combination index of 0.61 was achieved by treating PC-3 cells with betanin and RT. The results pointed out that betanin synergistically triggered RT-mediated apoptosis and cell cycle arrest through modulating gene and protein expression in comparison with each of the monotherapies. CONCLUSION: These findings shed light on the synergistic antitumor effect of betanin and RT in prostate cancer, indicating the potential use of betanin as a radiosensitizer agent.

Electron Paramagnetic Resonance Studies of Irradiated Grape Snails (Helix pomatia) and Investigation of Biophysical Parameters.

A study of grape snails (Helix pomatia) using the electron paramagnetic resonance (EPR) spectroscopy method, where shells were exposed to ionizing gamma radiation, indicated that the effect of radiation up to certain doses results in the emergence of magnetic properties in the organism. The identification of the EPR spectra of the body and shell parts of the control and irradiated grape snails separately showed that more iron oxide magnetic nanoparticles are generated in the body part of the grape snail compared to the shells. A linear increase in free radical signals (g = 2.0023) in the body and shell parts of grape snails, and a non-monotonic change in the broad EPR signal (g = 2.32) characterizing iron oxide magnetic nanoparticles was determined depending on the dose of ionizing gamma radiation. Additionally, the obtained results showed that grape snails can be used as bioindicators for examining the ecological state of the environment. At the same time, the radionuclide composition of the body and shell parts of the grape snails and their specific activities were determined by CANBERRA gamma spectroscopy. The FTIR spectra of mucin, a liquid secreted by snails, were recorded.

The effect of hyaluronic acid hydrogels on dental pulp stem cells behavior.

Dental caries and trauma, particularly in childhood, are among the most prevalent teeth problems, which result in the creation of cavities and probably tooth loss. Thus, novel regenerative approaches with high efficiency and less toxicity are required. Stem cell therapy along with the implementation of scaffolds has provided excellent opportunities in the regeneration of teeth structure. Hyaluronic acid (HA) hydrogels have enticed great attention in the field of regenerative medicine. The unique chemical and structural properties of HA and its derivatives have enabled their application in tissue engineering. Several factors such as the location and type of the lesion, teeth age, the type of capping materials determine the success rate of pulp therapy. HA hydrogels have been considered as biocompatible and safe scaffold supports in human dental cell therapies.

An overview application of silver nanoparticles in inhibition of herpes simplex virus.

Nanoscale particles and molecules are a potential different for the treatment of disease because they have distinctive biologic property based on their structure and size, which is different from traditional small-molecule drugs. The antimicrobial mechanisms of silver nanoparticles include the formation of free radicals damaging the bacterial membranes, interactions with DNA, adhesion to cell surface altering the membrane properties, and enzyme damage. In this review, we focus on applications of silver nanoparticles in inhibition of herpes simplex virus.

Synthesis and Evaluation of a Triblock Copolymer/ZnO Nanoparticles from Poly(ε-caprolactone) and Poly(Acrylic Acid) as a Potential Drug Delivery Carrier.

Through the present paper, a triblock copolymer containing pH-responsive (polyacrylic acid-b-polycaprolactone -b-polyacrylic acid) (PAA-b-PCL-b-PAA) was synthesized by using the ring-opening polymerization (ROP) of ε-caprolactone (ε-CL) and the reversible addition fragmentation chain transfer (RAFT) polymerization of the acrylic acid methods, as the drug carrier. Blends of the nanocrystalline zinc oxide nanoparticles (n-ZnO) and triblock copolymer treated from the solution have been used to make the hybrid polymer-metal oxide for the preparation of the drug loaded nanocomposite. The drug-release behavior of the nanocomposite was studied by using the Doxorubicin as a model drug. In addition to the self-assembly and pH-responsive behavior of the triblock copolymers/ZnO was studied in solution by the Fluorescence Spectroscopy, Scanning Electron Microscopy(SEM), Transmission Electron Microscopy (TEM), DLS, HNMR and FT-IR spectroscopy.