RESUMO
In this study we have investigated various morphofunctional features of gallbladder mucosa in patients with uncomplicated cholelithiasis. The histological changes, endocrine cell types and their distribution, and mucin-producing cells were characterized by immunocytochemistry and mucin histochemistry; moreover, we attempted to correlate these findings to the number and size of gallbladder stones and the type of bacteria present in the bile. Our results indicate that, despite similar clinical parameters, a wide range of histological changes can occur in the gallbladder mucosa of these patients. Moreover, the presence of certain endocrine and mucin-producing cell types in so-called "pyloric metaplasia" led us to hypothesize that this finding may be a trivial event in the gallbladder epithelium.
Assuntos
Colelitíase/patologia , Vesícula Biliar/patologia , Mucinas/biossíntese , Epitélio/patologia , Feminino , Vesícula Biliar/metabolismo , Humanos , Técnicas Imunoenzimáticas , Pessoa de Meia-Idade , Mucosa/patologia , Muco/metabolismoRESUMO
In this study the authors have investigated the different morphofunctional features of the gallbladder mucosa in patients with uncomplicated cholelithiasis. Histological changes, type and distribution of endocrine and mucin-producing cells were characterized by immunocytochemistry and mucin histochemistry. The authors attempted to correlate these findings to the number and size of gallbladder stones as well as type of bacteria present in the bile. The results indicate that, despite similar clinical parameters, a wide range of histological changes may occur in the gallbladder mucosa of these patients. Moreover, the presence of some endocrine and mucin-producing cell types in the so called "pyloric metaplasia" led the Authors to hypothesize that the latter may be a trivial event.
Assuntos
Colelitíase/patologia , Vesícula Biliar/citologia , Células Epiteliais , Epitélio/metabolismo , Epitélio/patologia , Feminino , Vesícula Biliar/metabolismo , Vesícula Biliar/patologia , Histocitoquímica , Humanos , Imuno-Histoquímica , Pessoa de Meia-Idade , Mucinas/análise , Mucosa/citologia , Mucosa/metabolismo , Mucosa/patologia , Sistemas Neurossecretores/citologiaRESUMO
A new, simplified procedure for identification of bacteria belonging to the genus Salmonella is reported. By this procedure which is a new combination of well know tests, Salmonella spp. are easily and reproducibly differentiated from non Salmonella spp. after 6 h of incubation at 37 degrees C.
