Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 36
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Front Immunol ; 10: 585, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31024522

RESUMO

The brain regulates physiological functions integral to survival. However, the insight into brain neuronal regulation of peripheral immune function and the neuromediator systems and pathways involved remains limited. Here, utilizing selective genetic and pharmacological approaches, we studied the role of forebrain cholinergic signaling in the regulation of peripheral immune function and inflammation. Forebrain-selective genetic ablation of acetylcholine release and vagotomy abolished the suppression of serum TNF by the centrally-acting cholinergic drug galantamine in murine endotoxemia. Selective stimulation of acetylcholine action on the M1 muscarinic acetylcholine receptor (M1 mAChR) by central administration of the positive allosteric modulator benzyl quinolone carboxylic acid (BQCA) suppressed serum TNF (TNFα) levels in murine endotoxemia. This effect was recapitulated by peripheral administration of the compound. BQCA also improved survival in murine endotoxemia and these effects were abolished in M1 mAChR knockout (KO) mice. Selective optogenetic stimulation of basal forebrain cholinergic neurons innervating brain regions with abundant M1 mAChR localization reduced serum TNF in endotoxemic mice. These findings reveal that forebrain cholinergic neurons regulate innate immune responses and inflammation, suggesting the possibility that in diseases associated with cholinergic dysfunction, including Alzheimer's disease this anti-inflammatory regulation can be impaired. These results also suggest novel anti-inflammatory approaches based on targeting forebrain cholinergic signaling in sepsis and other disorders characterized by immune dysregulation.


Assuntos
Prosencéfalo/imunologia , Receptor Muscarínico M1/imunologia , Acetilcolina/farmacologia , Animais , Anti-Inflamatórios/farmacologia , Agonistas Colinérgicos/farmacologia , Inibidores da Colinesterase/farmacologia , Citocinas/sangue , Citocinas/imunologia , Endotoxemia/imunologia , Endotoxemia/metabolismo , Galantamina/farmacologia , Imunidade Inata , Inflamação/imunologia , Inflamação/metabolismo , Masculino , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Prosencéfalo/metabolismo , Quinolinas/farmacologia , Receptor Muscarínico M1/genética , Receptor Muscarínico M1/metabolismo , Proteínas Vesiculares de Transporte de Acetilcolina/genética
2.
Invest Ophthalmol Vis Sci ; 59(7): 2778-2791, 2018 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-29860464

RESUMO

Purpose: Myopia is a refractive disorder that degrades vision. It can be treated with atropine, a muscarinic acetylcholine receptor (mAChR) antagonist, but the mechanism is unknown. Atropine may block α-adrenoceptors at concentrations ≥0.1 mM, and another potent myopia-inhibiting ligand, mamba toxin-3 (MT3), binds equally well to human mAChR M4 and α1A- and α2A-adrenoceptors. We hypothesized that mAChR antagonists could inhibit myopia via α2A-adrenoceptors, rather than mAChR M4. Methods: Human mAChR M4 (M4), chicken mAChR M4 (cM4), or human α2A-adrenergic receptor (hADRA2A) clones were cotransfected with CRE/promoter-luciferase (CRE-Luc; agonist-induced luminescence) and Renilla luciferase (RLuc; normalizing control) into human cells. Inhibition of normalized agonist-induced luminescence by antagonists (ATR: atropine; MT3; HIM: himbacine; PRZ: pirenzepine; TRP: tropicamide; OXY: oxyphenonium; QNB: 3-quinuclidinyl benzilate; DIC: dicyclomine; MEP: mepenzolate) was measured using the Dual-Glo Luciferase Assay System. Results: Relative inhibitory potencies of mAChR antagonists at mAChR M4/cM4, from most to least potent, were QNB > OXY ≥ ATR > MEP > HIM > DIC > PRZ > TRP. MT3 was 56× less potent at cM4 than at M4. Relative potencies of mAChR antagonists at hADRA2A, from most to least potent, were MT3 > HIM > ATR > OXY > PRZ > TRP > QNB > MEP; DIC did not antagonize. Conclusions: Muscarinic antagonists block hADRA2A signaling at concentrations comparable to those used to inhibit chick myopia (≥0.1 mM) in vivo. Relative potencies at hADRA2A, but not M4/cM4, correlate with reported abilities to inhibit chick form-deprivation myopia. mAChR antagonists might inhibit myopia via α2-adrenoceptors, instead of through the mAChR M4/cM4 receptor subtype.


Assuntos
Atropina/farmacologia , Antagonistas Muscarínicos/farmacologia , Miopia/prevenção & controle , Receptores Adrenérgicos alfa 2/metabolismo , Receptores Muscarínicos/metabolismo , Agonistas de Receptores Adrenérgicos alfa 2/farmacologia , Animais , Proteína 9 Associada à CRISPR , Carbacol/farmacologia , Galinhas , Agonistas Colinérgicos/farmacologia , Clonidina/farmacologia , Técnicas de Silenciamento de Genes , Células HEK293 , Humanos , Ligantes , Receptor Muscarínico M3/genética , Receptor Muscarínico M4/metabolismo , Transfecção
3.
Pharmacol Res Perspect ; 3(1): e00100, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25692018

RESUMO

One of the major signs of severe organophosphate poisoning is seizures. Previous studies have shown that both muscarinic agonist- and organophosphate-induced seizures require activation of muscarinic acetylcholine receptors in the central nervous system. Seizures induced by the muscarinic agonist pilocarpine require the M1 receptor and are modulated by cannabinoid CB1 receptors. In this study, we determined whether M1 and CB1 receptors also regulated seizures induced by the organophosphate paraoxon. We found no differences in seizures induced by paraoxon in wild-type (WT) and M1 knockout (KO) mice, indicating that in contrast to pilocarpine seizures, M1 receptors are not required for paraoxon seizures. Furthermore, we found that pilocarpine administration resulted in seizure-independent activation of ERK in the hippocampus in a M1 receptor-dependent manner, while paraoxon did not induce seizure-independent activation of ERK in the mouse hippocampus. This shows that pilocarpine and paraoxon activated M1 receptors in the hippocampus to different extents. There were no differences in seizures induced by paraoxon in WT and CB1 KO mice, and neither CB1 agonist nor antagonist administration had significant effects on paraoxon seizures, indicating that, in contrast to pilocarpine seizures, paraoxon seizures are not modulated by CB1 receptors. These results demonstrate that there are fundamental molecular differences in the regulation of seizures induced by pilocarpine and paraoxon.

4.
Ann Clin Transl Neurol ; 1(11): 926-32, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25540807

RESUMO

A woman was isozygous for a novel mutation in the leukemia inhibitory factor receptor gene (LIFR) (c.2170C>G; p.Pro724Ala) which disrupts LIFR downstream signaling and results in Stüve-Wiedemann syndrome (STWS). She inherited two identical chromosomes 5 from her mother, heterozygous for the LIFR mutation. The presentation was typical for STWS, except there was no long bone dysplasia. Prominent cold-induced sweating and heat intolerance lead to an initial diagnosis of cold-induced sweating syndrome, excluded by exome sequencing. Skin biopsies provide the first human evidence of failed postnatal cholinergic differentiation of sympathetic neurons innervating sweat glands in cold-induced sweating, and of a neuropathy.

5.
J Physiol ; 592(16): 3463-94, 2014 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-24879872

RESUMO

Parvalbumin-containing (PV) neurons, a major class of GABAergic interneurons, are essential circuit elements of learning networks. As levels of acetylcholine rise during active learning tasks, PV neurons become increasingly engaged in network dynamics. Conversely, impairment of either cholinergic or PV interneuron function induces learning deficits. Here, we examined PV interneurons in hippocampus (HC) and prefrontal cortex (PFC) and their modulation by muscarinic acetylcholine receptors (mAChRs). HC PV cells, visualized by crossing PV-CRE mice with Rosa26YFP mice, were anatomically identified as basket cells and PV bistratified cells in the stratum pyramidale; in stratum oriens, HC PV cells were electrophysiologically distinct from somatostatin-containing cells. With glutamatergic transmission pharmacologically blocked, mAChR activation enhanced PV cell excitability in both CA1 HC and PFC; however, CA1 HC PV cells exhibited a stronger postsynaptic depolarization than PFC PV cells. To delete M1 mAChRs genetically from PV interneurons, we created PV-M1 knockout mice by crossing PV-CRE and floxed M1 mice. The elimination of M1 mAChRs from PV cells diminished M1 mAChR immunoreactivity and muscarinic excitation of HC PV cells. Selective cholinergic activation of HC PV interneurons using Designer Receptors Exclusively Activated by Designer Drugs technology enhanced the frequency and amplitude of inhibitory synaptic currents in CA1 pyramidal cells. Finally, relative to wild-type controls, PV-M1 knockout mice exhibited impaired novel object recognition and, to a lesser extent, impaired spatial working memory, but reference memory remained intact. Therefore, the direct activation of M1 mAChRs on PV cells contributes to some forms of learning and memory.


Assuntos
Potenciais de Ação , Cognição , Potenciais Pós-Sinápticos Inibidores , Interneurônios/metabolismo , Receptor Muscarínico M1/metabolismo , Animais , Região CA1 Hipocampal/citologia , Região CA1 Hipocampal/metabolismo , Região CA1 Hipocampal/fisiologia , Feminino , Interneurônios/fisiologia , Masculino , Camundongos , Parvalbuminas/genética , Parvalbuminas/metabolismo , Córtex Pré-Frontal/citologia , Córtex Pré-Frontal/metabolismo , Córtex Pré-Frontal/fisiologia , Receptor Muscarínico M1/genética
6.
PLoS One ; 9(4): e95922, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24752144

RESUMO

Administration of the muscarinic agonist pilocarpine is commonly used to induce seizures in rodents for the study of epilepsy. Activation of muscarinic receptors has been previously shown to increase the production of endocannabinoids in the brain. Endocannabinoids act at the cannabinoid CB1 receptors to reduce neurotransmitter release and the severity of seizures in several models of epilepsy. In this study, we determined the effect of CB1 receptor activity on the induction in mice of seizures by pilocarpine. We found that decreased activation of the CB1 receptor, either through genetic deletion of the receptor or treatment with a CB1 antagonist, increased pilocarpine seizure severity without modifying seizure-induced cell proliferation and cell death. These results indicate that endocannabinoids act at the CB1 receptor to modulate the severity of pilocarpine-induced seizures. Administration of a CB1 agonist produced characteristic CB1-dependent behavioral responses, but did not affect pilocarpine seizure severity. A possible explanation for the lack of effect of CB1 agonist administration on pilocarpine seizures, despite the effects of CB1 antagonist administration and CB1 gene deletion, is that muscarinic receptor-stimulated endocannabinoid production is acting maximally at CB1 receptors to modulate sensitivity to pilocarpine seizures.


Assuntos
Agonistas Muscarínicos/farmacologia , Pilocarpina/farmacologia , Receptor CB1 de Canabinoide/metabolismo , Convulsões/induzido quimicamente , Convulsões/metabolismo , Animais , Cicloexanóis/farmacologia , Masculino , Camundongos , Camundongos Knockout , Antígeno Nuclear de Célula em Proliferação/genética , Antígeno Nuclear de Célula em Proliferação/metabolismo , Receptor CB1 de Canabinoide/genética
8.
Neurochem Int ; 61(6): 874-8, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22306348

RESUMO

Leukemia inhibitory factor (LIF) and ciliary neurotrophic factor (CNTF) are neurally active cytokines, or neurokines. LIF signals through a receptor consisting of gp130 and the low affinity LIF receptor (LIFR), while the CNTF receptor consists of gp130, LIFR, and the low affinity CNTF receptor (CNTFR). Ser1044 of the LIFR is phosphorylated by Erk1/2 MAP kinase. Stimulation of neural cells with growth factors which strongly activate Erk1/2 decreases LIF-mediated signal transduction due to increased degradation of the LIFR as a consequence of Erk1/2-dependent phosphorylation of the receptor at Ser1044. The gp130 receptor subunit is phosphorylated, at least in part by calmodulin-dependent protein kinase II, at Ser782, which is adjacent to a dileucine internalization motif. Ser782 appears to negatively regulate cytokine receptor expression, as mutagenesis of Ser782 results in increased gp130 expression and cytokine-induced neuropeptide gene transcription. The LIFR and gp130 are transmembrane proteins, while CNTFR is a peripheral membrane protein attached to the cell surface via a glycosylphosphatidylinositol tail. In unstimulated cells, CNTFR but not LIFR and gp130 is localized to detergent-resistant lipid rafts. Stimulation of cells with CNTFR causes translocation of LIFR and gp130 into the lipid rafts, while stimulation with LIF does not induce receptor translocation, raising the possibility that CNTF could induce different patterns of signaling and/or receptor trafficking than caused by LIF. We used a compartmentalized culture system to examine the mechanisms for retrograde signaling by LIF and CNTF from distal neurites to the cell bodies of mouse sympathetic neurons. Stimulation with neurokines of the distal neurites of sympathetic neurons grown in a compartmentalized culture system resulted in the activation and nuclear translocation of the transcription factor Stat3. Retrograde signaling required Jak kinase activity in the cell body but not the distal neurites, and could be blocked by inhibitors of microtubule but not microfilament function. The results are consistent with a signaling endosomes model in which the ctyokine/receptor complex is transported back to the cell body where Stat3 is activated. While both LIF and CNTF mediate retrograde activation of Stat3, the kinetics for retrograde signaling differ for the two neurokines.


Assuntos
Transporte Proteico , Receptor do Fator Neutrófico Ciliar/metabolismo , Receptores de OSM-LIF/metabolismo , Transdução de Sinais , Animais , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Humanos , Fosforilação
9.
Handb Exp Pharmacol ; (208): 61-78, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22222695

RESUMO

Knowledge of the mechanisms responsible for the trafficking of neurotransmitter receptors away from the cell surface is of obvious importance in understanding what regulates their expression and function. This chapter will focus on the mechanisms responsible for the internalization and degradation of muscarinic receptors. There are both receptor subtype-specific and cell-type specific differences in muscarinic receptor trafficking. Studies on muscarinic receptor trafficking both in cells in culture and in vivo will be described, and the potential physiological consequences of this trafficking will be discussed.


Assuntos
Membrana Celular/metabolismo , Endocitose , Receptores Muscarínicos/metabolismo , Acetilcolinesterase/deficiência , Acetilcolinesterase/genética , Animais , Proteínas Ligadas por GPI/deficiência , Proteínas Ligadas por GPI/genética , Humanos , Ligantes , Camundongos , Camundongos Knockout , Proteínas Monoméricas de Ligação ao GTP/metabolismo , Multimerização Proteica , Processamento de Proteína Pós-Traducional , Transporte Proteico
10.
PLoS One ; 5(10): e13517, 2010 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-20976005

RESUMO

Receptor internalization from the cell surface occurs through several mechanisms. Some of these mechanisms, such as clathrin coated pits, are well understood. The M(2) muscarinic acetylcholine receptor undergoes internalization via a poorly-defined clathrin-independent mechanism. We used isotope coded affinity tagging and mass spectrometry to identify the scaffolding protein, receptor for activated C kinase (RACK1) as a protein enriched in M(2)-immunoprecipitates from M(2)-expressing cells over those of non-M(2) expressing cells. Treatment of cells with the agonist carbachol disrupted the interaction of RACK1 with M(2). We further found that RACK1 overexpression inhibits the internalization and subsequent down regulation of the M(2) receptor in a receptor subtype-specific manner. Decreased RACK1 expression increases the rate of agonist internalization of the M(2) receptor, but decreases the extent of subsequent down-regulation. These results suggest that RACK1 may both interfere with agonist-induced sequestration and be required for subsequent targeting of internalized M(2) receptors to the degradative pathway.


Assuntos
Proteínas de Ligação ao GTP/metabolismo , Proteínas de Neoplasias/metabolismo , Receptor Muscarínico M2/metabolismo , Receptores de Superfície Celular/metabolismo , Marcadores de Afinidade , Eletroforese em Gel de Poliacrilamida , Humanos , Espectrometria de Massas , Transporte Proteico , Receptores de Quinase C Ativada
11.
J Neurosci ; 30(19): 6751-62, 2010 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-20463237

RESUMO

Acetylcholine is the major neurotransmitter of the olivocochlear efferent system, which provides feedback to cochlear hair cells and sensory neurons. To study the role of cochlear muscarinic receptors, we studied receptor localization with immunohistochemistry and reverse transcription-PCR and measured olivocochlear function, cochlear responses, and histopathology in mice with targeted deletion of each of the five receptor subtypes. M2, M4, and M5 were detected in microdissected immature (postnatal days 10-13) inner hair cells and spiral ganglion cells but not outer hair cells. In the adult (6 weeks), the same transcripts were found in microdissected organ of Corti and spiral ganglion samples. M2 protein was found, by immunohistochemistry, in olivocochlear fibers in both outer and inner hair cell areas. M3 mRNA was amplified only from whole cochleas, and M1 message was never seen in wild-type ears. Auditory brainstem responses (ABRs) and distortion product otoacoustic emissions (DPOAEs) were unaffected by loss of Gq-coupled receptors (M1, M3, or M5), as were shock-evoked olivocochlear effects and vulnerability to acoustic injury. In contrast, loss of Gi-coupled receptors (M2 and/or M4) decreased neural responses without affecting DPOAEs (at low frequencies). This phenotype and the expression pattern are consistent with excitatory muscarinic signaling in cochlear sensory neurons. At high frequencies, both ABRs and DPOAEs were attenuated by loss of M2 and/or M4, and the vulnerability to acoustic injury was dramatically decreased. This aspect of the phenotype and the expression pattern are consistent with a presynaptic role for muscarinic autoreceptors in decreasing ACh release from olivocochlear terminals during high-level acoustic stimulation and suggest that muscarinic antagonists could enhance the resistance of the inner ear to noise-induced hearing loss.


Assuntos
Cóclea/fisiologia , Neurônios Aferentes/fisiologia , Neurônios Eferentes/fisiologia , Terminações Pré-Sinápticas/fisiologia , Receptores Muscarínicos/metabolismo , Animais , Limiar Auditivo/fisiologia , Cóclea/crescimento & desenvolvimento , Potenciais Evocados Auditivos do Tronco Encefálico , Retroalimentação Fisiológica , Feminino , Células Ciliadas Auditivas Internas/fisiologia , Células Ciliadas Auditivas Externas/fisiologia , Perda Auditiva Provocada por Ruído/fisiopatologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Emissões Otoacústicas Espontâneas , RNA Mensageiro/metabolismo , Receptores Muscarínicos/genética , Gânglio Espiral da Cóclea/crescimento & desenvolvimento , Gânglio Espiral da Cóclea/fisiologia , Transmissão Sináptica/fisiologia
12.
Hippocampus ; 20(4): 492-8, 2010 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19557767

RESUMO

Activity-dependent changes in gene-expression are believed to underlie the molecular representation of memory. In this study, we report that in vivo activation of neurons rapidly induces the CREB-regulated microRNA miR-132. To determine if production of miR-132 is regulated by neuronal activity its expression in mouse brain was monitored by quantitative RT-PCR (RT-qPCR). Pilocarpine-induced seizures led to a robust, rapid, and transient increase in the primary transcript of miR-132 (pri-miR-132) followed by a subsequent rise in mature microRNA (miR-132). Activation of neurons in the hippocampus, olfactory bulb, and striatum by contextual fear conditioning, odor-exposure, and cocaine-injection, respectively, also increased pri-miR-132. Induction kinetics of pri-miR-132 were monitored and found to parallel those of immediate early genes, peaking at 45 min and returning to basal levels within 2 h of stimulation. Expression levels of primary and mature-miR-132 increased significantly between postnatal Days 10 and 24. We conclude that miR-132 is an activity-dependent microRNA in vivo, and may contribute to the long-lasting proteomic changes required for experience-dependent neuronal plasticity.


Assuntos
Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/genética , MicroRNAs/genética , Plasticidade Neuronal/genética , Neurônios/fisiologia , Convulsões/genética , Transcrição Gênica/genética , Animais , Aprendizagem por Associação/fisiologia , Comportamento Animal/fisiologia , Expressão Gênica/genética , Hipocampo/fisiologia , Masculino , Camundongos , Pilocarpina , RNA Mensageiro/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Convulsões/induzido quimicamente
13.
J Neurosci ; 29(12): 3843-51, 2009 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-19321781

RESUMO

Cisplatin is a chemotherapy drug that frequently causes auditory impairment due to the death of mechanosensory hair cells. Cisplatin ototoxicity may result from oxidative stress, DNA damage, and inflammatory cytokines. The transcription factor STAT1, an important mediator of cell death, can regulate all of these processes in other cell types. We used cultured utricles from mature Swiss Webster mice to investigate the role of STAT1 in cisplatin-induced hair cell death. We show that STAT1 phosphorylation is an early event in both hair cells and support cells after exposure of utricles to cisplatin. STAT1 phosphorylation peaked after 4 h of cisplatin exposure and returned to control levels by 8 h of exposure. The STAT1 inhibitor epigallocatechin gallate (EGCG) attenuated STAT1 phosphorylation in cisplatin-treated utricles and resulted in concentration-dependent increases in hair cell survival at 24 h postexposure. Furthermore, we show that utricular hair cells from STAT1-deficient mice are resistant to cisplatin toxicity. EGCG failed to provide additional protection from cisplatin in STAT1-deficient mice, further supporting the hypothesis that the protective effects of EGCG are due to its inhibition of STAT1. Treatment with IFN-gamma, which also causes STAT1 activation, also induced hair cell death in wild-type but not STAT1-deficient mice. These results show that STAT1 is required for maximal cisplatin-induced hair cell death in the mouse utricle and suggest that treatment with EGCG may be a useful strategy for prevention of cisplatin ototoxicity.


Assuntos
Antineoplásicos/toxicidade , Catequina/análogos & derivados , Cisplatino/toxicidade , Células Ciliadas Auditivas/efeitos dos fármacos , Fator de Transcrição STAT1/fisiologia , Animais , Catequina/farmacologia , Morte Celular , Células Ciliadas Auditivas/citologia , Interferon gama/farmacologia , Camundongos , Camundongos Knockout , Fosforilação , Fator de Transcrição STAT1/genética , Fator de Transcrição STAT1/metabolismo , Sáculo e Utrículo/citologia , Sáculo e Utrículo/efeitos dos fármacos , Sáculo e Utrículo/metabolismo , Serina/metabolismo , Técnicas de Cultura de Tecidos
14.
J Neurochem ; 106(4): 1941-51, 2008 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-18624908

RESUMO

The cytokines that signal through the leukemia inhibitory factor (LIF) receptor are members of the neuropoietic cytokine family and have varied and numerous roles in the nervous system. In this report, we have determined the effects of growth factor stimulation on LIF receptor (LIFR) expression and signal transduction in the human neuroblastoma cell line NBFL. We show here that stimulation of NBFL cells with either epidermal growth factor or fibroblast growth factor decreases the level of LIFR in an extracellular signal-regulated kinase (Erk)1/2-dependent manner and that this down-regulation is due to an increase in the apparent rate of lysosomal LIFR degradation. Growth factor-induced decreases in LIFR level inhibit both LIF-stimulated phosphorylation of signal transducers and activators of transcription 3 and LIFR-mediated gene induction. We also show that Ser1044 of LIFR, which we have previously shown to be phosphorylated by Erk1/2, is required for the inhibitory effects of growth factors. Neurons are exposed to varying combinations and concentrations of growth factors and cytokines that influence their growth, development, differentiation, and repair in vivo. These findings demonstrate that LIFR expression and signaling in neuroblastoma cells can be regulated by growth factors that are potent activators of the mitogen-activated protein kinase pathway, and thus illustrate a fundamental mechanism that underlies crosstalk between receptor tyrosine kinase and neuropoietic cytokine signaling pathways.


Assuntos
Regulação Neoplásica da Expressão Gênica/fisiologia , Peptídeos e Proteínas de Sinalização Intercelular/farmacologia , Neuroblastoma/metabolismo , Receptores de OSM-LIF/biossíntese , Receptores de OSM-LIF/genética , Transdução de Sinais/fisiologia , Animais , Linhagem Celular Tumoral , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Camundongos , Ratos , Receptor Cross-Talk/fisiologia , Receptores de OSM-LIF/metabolismo , Receptores de OSM-LIF/fisiologia , Transdução de Sinais/efeitos dos fármacos , Ativação Transcricional
15.
Pharmacol Ther ; 119(1): 33-43, 2008 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-18558434

RESUMO

Muscarinic acetylcholine receptors are members of the G-protein coupled receptor superfamily that are expressed in and regulate the function of neurons, cardiac and smooth muscle, glands, and many other cell types and tissues. The correct trafficking of membrane proteins to the cell surface and their subsequent localization at appropriate sites in polarized cells are required for normal cellular signaling and physiological responses. This review will summarize work on the synthesis and trafficking of muscarinic receptors to the plasma membrane and their localization at the cell surface.


Assuntos
Receptores Muscarínicos/metabolismo , Animais , Encéfalo/metabolismo , Glicosilação , Humanos , Mucosa Intestinal/metabolismo , Rim/metabolismo , Proteínas de Membrana/biossíntese , Oócitos/metabolismo , Transporte Proteico , Receptores Muscarínicos/análise
16.
Life Sci ; 82(13-14): 718-27, 2008 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-18295803

RESUMO

Multiple mechanisms exist for the endocytosis of receptors from the cell surface. While the M1, M3, and M4 subtypes of muscarinic acetylcholine receptor and M4 receptors transduce their signals through the same second messengers but internalize though different pathways, we tested the ability of several small G-proteins to regulate the agonist-induced endocytosis of M2 and M4 in JEG-3 human choriocarcinoma cells. Dominant-negative Rab5 as well as both wild-type and dominant-negative Rab11 inhibited M4 but not M2 endocytosis. In contrast, a dominant-negative Arf6 as well as wild-type Rab22 increased M2 but not M4 endocytosis. We used immunocytochemistry to show that in unstimulated cells, the M2 and M4 receptors co-localize on the cell surface, whereas after stimulation M2 and M4 are in distinct vesicular compartments. In this study, we demonstrate that agonist-induced internalization of the M2 receptor utilizes an Arf6, Rab22 dependent pathway, while the M4 receptor undergoes agonist-induced internalization through a Rab5, Rab11 dependent pathway. Additionally, we show that Rab15 and RhoA are not involved in either pathway in JEG-3 cells.


Assuntos
Endocitose/fisiologia , Proteínas de Ligação ao GTP/fisiologia , Receptor Muscarínico M2/metabolismo , Receptor Muscarínico M4/metabolismo , Técnicas de Cultura de Células , Linhagem Celular Tumoral , Proteínas de Ligação ao GTP/genética , Humanos , Microscopia Confocal , Ligação Proteica , Subunidades Proteicas , Transporte Proteico , Transdução de Sinais/fisiologia , Transfecção , Vesículas Transportadoras/metabolismo
17.
Nat Neurosci ; 10(11): 1458-66, 2007 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-17906621

RESUMO

Dopamine-depleting lesions of the striatum that mimic Parkinson's disease induce a profound pruning of spines and glutamatergic synapses in striatopallidal medium spiny neurons, leaving striatonigral medium spiny neurons intact. The mechanisms that underlie this cell type-specific loss of connectivity are poorly understood. The Kir2 K(+) channel is an important determinant of dendritic excitability in these cells. Here we show that opening of these channels is potently reduced by signaling through M1 muscarinic receptors in striatopallidal neurons, but not in striatonigral neurons. This asymmetry could be attributed to differences in the subunit composition of Kir2 channels. Dopamine depletion alters the subunit composition further, rendering Kir2 channels in striatopallidal neurons even more susceptible to modulation. Reduced opening of Kir2 channels enhances dendritic excitability and synaptic integration. This cell type-specific enhancement of dendritic excitability is an essential trigger for synaptic pruning after dopamine depletion, as pruning was prevented by genetic deletion of M1 muscarinic receptors.


Assuntos
Corpo Estriado/citologia , Dendritos/fisiologia , Neurônios/ultraestrutura , Canais de Potássio Corretores do Fluxo de Internalização/fisiologia , Receptor Muscarínico M1/fisiologia , Animais , Animais Recém-Nascidos , Inibidores Enzimáticos/farmacologia , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Potenciais Pós-Sinápticos Excitadores/fisiologia , Proteínas de Fluorescência Verde/metabolismo , Técnicas In Vitro , Potenciais da Membrana/efeitos dos fármacos , Potenciais da Membrana/fisiologia , Potenciais da Membrana/efeitos da radiação , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Muscarina/farmacologia , Agonistas Muscarínicos/farmacologia , Neurônios/citologia , Neurônios/efeitos dos fármacos , Neurônios/efeitos da radiação , Oxidopamina/farmacologia , Técnicas de Patch-Clamp/métodos , Receptor Muscarínico M1/deficiência , Receptores de Dopamina D1/metabolismo , Receptores de Dopamina D2/metabolismo , Reserpina/farmacologia , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/fisiologia
18.
J Neurochem ; 103(1): 288-302, 2007 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-17608645

RESUMO

Leukemia inhibitory factor (LIF) is a member of the interleukin-6 family of cytokines and signals through the glycoprotein 130 and LIF receptor beta subunits. Binding of cytokines to these subunits activates multiple signaling cascades, including the Janus kinase (Jak)/signal transducers and activators of transcription (STAT) pathway. We used compartmentalized cultures of sympathetic neurons and immunocytochemical analyses of STAT3 to examine the mechanisms involved in retrograde signaling of LIF from distal neurites (DN) to cell bodies. Addition of LIF to the DN of these neurons triggers the activation and nuclear translocation of STAT3. Inhibition of Jak activity in the cell bodies prevented LIF-induced retrograde activation of STAT3, while block of Jak activity in the DN had no effect on the appearance of activated STAT3 in the nucleus. These results show that the transport of activated Jak is not the main mechanism mediating retrograde signaling. Although there is an increase in phosphorylated STAT3 in the neurites after distal stimulation, the transport of activated STAT3 is not necessary for retrograde signaling. Our results are consistent with a signaling endosome model for retrograde signaling, in which the LIF/glycoprotein 130/LIF receptor/Jak complex is internalized and transported to activate STAT3 in the cell body.


Assuntos
Fator Inibidor de Leucemia/fisiologia , Neurônios/metabolismo , Fator de Transcrição STAT3/metabolismo , Transdução de Sinais/fisiologia , Transporte Ativo do Núcleo Celular/efeitos dos fármacos , Animais , Núcleo Celular/metabolismo , Células Cultivadas , Fator Inibidor de Leucemia/farmacologia , Camundongos , Camundongos Endogâmicos C57BL , Neuritos/efeitos dos fármacos , Neuritos/metabolismo , Neurônios/efeitos dos fármacos , Fator de Transcrição STAT3/efeitos dos fármacos , Transdução de Sinais/efeitos dos fármacos , Gânglio Cervical Superior/citologia
19.
J Neurochem ; 101(3): 782-93, 2007 May.
Artigo em Inglês | MEDLINE | ID: mdl-17448148

RESUMO

Leukemia inhibitory factor (LIF) and ciliary neurotrophic factor (CNTF) are cytokines which signal through receptor complexes that include the receptor subunits glycoprotein 130 (gp130) and the LIF receptor (LIFR), but CNTF also requires the non-signal transducing CNTF receptor (CNTFR) for binding. We show here that in IMR-32 neuronal cells endogenously expressing the receptor subunits for LIF and CNTF, CNTFR, but not gp130 or LIFR, is found in detergent-resistant lipid rafts. In addition, stimulation of these cells with CNTF resulted in a rapid translocation of a portion of gp130 and LIFR into detergent-resistant lipid rafts while an equivalent stimulation with LIF did not. Disruption of lipid rafts by cholesterol depletion of cell membranes blocked the CNTF-induced translocation of LIFR and gp130. Interestingly, while cholesterol-depletion did not inhibit signal transducer and activator of transcription 3 phosphorylation by either CNTF or LIF stimulation, it strongly inhibited both CNTF- and LIF-mediated phosphorylation of extracellular signal-regulated kinases 1 and 2 and Akt. LIF and CNTF generally appear to have redundant effects in cells responsive to both cytokines. Intriguingly, the data presented here suggest a possible mechanism whereby CNTF or other cytokines that signal through CNTFR could generate signals distinct from those elicited by cytokines such as LIF which utilize a LIFR/gp130 heterodimer, via association with or exclusion from lipid rafts.


Assuntos
Interleucina-6/metabolismo , Microdomínios da Membrana/metabolismo , Moléculas de Adesão de Célula Nervosa/metabolismo , Receptores de OSM-LIF/metabolismo , Transdução de Sinais/fisiologia , Linhagem Celular Tumoral , Fator Neurotrófico Ciliar/farmacologia , Contactinas , Interações Medicamentosas , Inibidores Enzimáticos/farmacologia , Humanos , Imunoprecipitação , Fator Inibidor de Leucemia/farmacologia , Microdomínios da Membrana/efeitos dos fármacos , Neuroblastoma/patologia , Transdução de Sinais/efeitos dos fármacos , Fatores de Tempo , beta-Ciclodextrinas/farmacologia
20.
J Biol Chem ; 281(46): 35381-96, 2006 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-16968700

RESUMO

Previous studies have shown that the M2 receptor is localized at steady state to the apical domain in Madin-Darby canine kidney (MDCK) epithelial cells. In this study, we identify the molecular determinants governing the localization and the route of apical delivery of the M2 receptor. First, by confocal analysis of a transiently transfected glycosylation mutant in which the three putative glycosylation sites were mutated, we determined that N-glycans are not necessary for the apical targeting of the M2 receptor. Next, using a chimeric receptor strategy, we found that two independent sequences within the M2 third intracellular loop can confer apical targeting to the basolaterally targeted M4 receptor, Val270-Lys280 and Lys280-Ser350. Experiments using Triton X-100 extraction followed by OptiPrep density gradient centrifugation and cholera toxin beta-subunit-induced patching demonstrate that apical targeting is not because of association with lipid rafts. 35S-Metabolic labeling experiments with domain-specific surface biotinylation as well as immunocytochemical analysis of the time course of surface appearance of newly transfected confluent MDCK cells expressing FLAG-M2-GFP demonstrate that the M2 receptor achieves its apical localization after first appearing on the basolateral domain. Domain-specific application of tannic acid of newly transfected cells indicates that initial basolateral plasma membrane expression is required for subsequent apical localization. This is the first demonstration that a G-protein-coupled receptor achieves its apical localization in MDCK cells via transcytosis.


Assuntos
Receptor Muscarínico M2/química , Receptor Muscarínico M2/metabolismo , Motivos de Aminoácidos , Animais , Linhagem Celular , Cães , Regulação da Expressão Gênica , Microdomínios da Membrana/metabolismo , Camundongos , Mutação , Transporte Proteico , Receptor Muscarínico M2/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...