Limited resection of lung cancer.

AIM: Follow-up results after a limited resection (segmentectomy, wedge resection of lung) are represented in this paper according to the TNM classification and histological type of tumour. METHODS: Since 1980 until 1997, 1173 patients (pts) with lung cancer were treated surgically. 42 (36 males and 6 females) of them underwent a limited resection of lung. Distribution of pts according to the TNM classification was: T1N0 was found in 12 pts and T2N0 in 11 pts, T2N0 in 11pts, T2N1 in 7 pts, other 12 pts had N2. Sqamous cell carcinoma was in 19 pts, Adenocarcinoma in 13 pts, Small cell carcinoma in 9 pts, one pt--Carcinosarcoma. After the operation 9 pts received radiation therapy, 9 pts--chemotherapy. RESULTS: The best follow-up results were in pts with T1-2N0 who received adjuvant therapy: median survival was 45.7 months. The pts, treated only surgically, lived 36.7 months. The pts with N2 lived only 9 months. CONCLUSION: 1. A limited resection of lung with lymph nodes dissection can be performed only in cases T1-2N0. 2. In cases with N0 of undifferentiated carcinoma (anaplastic, small cell) adjuvant therapy ought to be given after operation. 3. In cases of N1, N2 we recommend adjuvant therapy regardless of histological type of tumour.

Differential effects of vasopressin on the water, calcium and lysosomal enzyme contents of mitochondria-rich and lysosome-rich (granular) epithelial cells isolated from bullfrog urinary bladder.

Treatment of bullfrog urinary bladder with arginine vasopressin (AVP) elicited a dose-dependent increase in the basal movement of water and sodium across isolated tissues. Epithelial cells from the mucosal surface and incubated with 10 mU AVP/ml for 30 min retained a greater amount of intracellular water and calcium than cells not treated with hormone. The epithelial cells were further separated into two major fractions by density gradient centrifugation; cells damaged during these manipulations were separated from viable cells and discarded. Morphologcal examination of the two respective fractions indicated that they largely consisted of mitochondria-rich (MR) and granular (G) cell types which line the lumen of bullfrog bladder. The calcium content of MR cells averaged 25% greater than that of G-type cells. G cells had a markedly higher content of the characteristic lysosomal hydrolases, acid phsophatase and cathepsin B1, than that found in MR cells. Incubation of G cells with AVP elicited significant increments in water and calcium contents and extracellular release of lysosomal enzymes as compared to untreated cells. Among MR cells treated with AVP, cell calcium declines slightly but no significant increase in water content or extracellular hydrolase activity was detected in comparison with paired control cells. The physiological significance of acid proteinase release from G cells treated with AVP was evaluated in experiments with intact bladder. Proteinase inhibitors which suppress the activity of cathepsin B1 selectively antagonized the action of hormone on water permeation. The data suggest that alterations in the calcium and lysosomal hydrolase activity associated with G cells exposed to AVP may contribute to the hormone-induced water flow observed across the intact epithelium.

Surface modifications evoked by antidiuretic hormone in isolated epithelial cells: evidence from lectin probes.

Epithelial cells (80-90% "granular" type) were isolated from urinary bladders of Bufo marinus and Rana catesbiana. The inhibitory effect of alpha-methyl-D-mannoside on fluorescein-labeled concanavalin A (Con A) binding to these cells indicates that they possess specific binding sites for Con A. The lectin also mediates adsorption of erythrocytes to these cells. Both Con A binding and Con A-mediated hemadsorption to epithelial cells are depressed at 4 degrees C, as compared with cells maintained at 22 degrees C. Elevation of temperature to 37 degrees C, however, enhances hemadsorption independently of alterations in lectin binding. Treatment of cells with antidiuretic hormone (ADH) at 22 degrees C followed by 15 min of incubation at 22 degrees or 37 degrees C before exposure of cells to Con A promotes increments in Con A-mediated hemadsorption, but not in lectin binding, at 22 degrees or 37 degrees C. These hormonal effects are not significant when hemadsorption is assayed at 4 degrees C. Treatment of cells with another octapeptide, angiotensin, elicits a small, but significant, increment in hemadsorption to epithelial cells which is likewise uninfluenced by quantitative changes in lectin binding. Collectively, these data and other independent observations suggest that treatment with octapeptide hormones acts to enhance the redistribution and aggregation of lectin-binding proteins in the membranes of granular epithelial cells from amphibian urinary bladder. Such changes, in turn, may contribute to the alterations in membrane transport properties which characterize the hormonal response.