[Effect of External Irradiation and Immobilization Stress on the Reproductive System of Male Rats].

We studied the state of the reproductive system of male rats after irradiation at a dose of 2.0 Gy, immobilization stress (6 hours/day for 7 days) and their combined effects. On the 30th day after the combined treatment (37 days after irradiation) a decrease in the testicular weight by almost 50% compared with the control and lesions connected with the process of spermatogenesis are observed. In the remote period--on the 60th day (67th after irradiation) the effect of irradiation and irradiation in combination with immobilization stress leads to a sharp drop in the number of epididymal sperm (up to 18% of the control), and a reduction of their viability. The reaction ofthe reproductive system to the immobilization stress is expressed in a certain increase in the mass of the testes and epididymis, moderate imbalances in the composition of spermatogenic cells in the testis tissue, and in the long term--in the increased number of epididymal sperm and the decrease in their viability. Changes of testosterone in the blood serum, especially significant for the combined effect, reflect impairments of the regulation of the reproductive system of males under these conditions. With regard to individual indicators of the reproductive system of male rats in some cases, the- combined effects of radiation and stress had a synergistic, or, on the contrary, antagonistic character.

[State of the reproductive systemin in male rats of 1st generation obtained from irradiated parents and exposed to electromagnetic radiation (897 MHz) during embryogenesis and postnatal development].

The consequences of prolonged exposure to electromagnetic radiation from cellular phone (897 MHz, daily 8 h/day) in male rats of the 1st generation obtained from irradiated parents and subjected to prolonged exposure to electromagnetic radiation of the range of mobile communications during ontogeny and postnatal development were studied. It has been found that irradiation causes a decrease in the number of births of animals, changing the sex ratio towards the increase in the number of males. It had a significant impact on the reproductive system of males, accelerating their sexual development, revealed at the age of two months. Radiation from cell phones led to significant disproportions in the cell number at different stages of spermatogenesis. It increased the number of mature spermatozoa which decreased viability.

[Effect of radio-ecological conditions in the ChNPP exclusion zone on morphofunctional state and responsiveness of organs and tissues of laboratory animals].

The state of hematopoietic, reproductive and endocrine systems of the organisms of male rats and their offspring in generations (F0-F1-F2) was studied, and the sensitivity of an organism to the action of carcinogen (Af mice) after a stay in the ChNPP exclusion zone was analyzed. It was ascertained that the most significant changes of the morphofunctional state of the animals were observed in the II generation (F2), which remained for a long period under the conditions of radioactive contamination. We have revealed an increased number of leukocytes, neutrophils, lymphocytes, and, especially, monocytes as against while the decrease in the number of erythrocytes and haemoglobin content; the decrease of thyroid function and cortical layer of the adrenals as opposed to while the increase in the relative weight of testes and their epididymides and the decrease in the number of spermatocytes and spermatozoa in the testis tissue. The exposure of Af mice in the exclusion zone increases the processes of mutagenesis and carcinogenesis, and changes the organism response to standardized action of chemical carcinogen. The increase in the exposure time of animals intensifies metabolic processes in a cell and increases their sensitivity to the action of xenobiotics.

[Cytosol reception of estradiol and nuclear acception of estrogen-receptor complexes in target organs of female rats after gamma-irradiation]. / Tsitozol'naia retseptsiia éstradiola i iadernaia aktseptsiia éstrogenretseptornykh kompleksov vorganakh-misheniakh gamma-obluchennykh samok krys.

Condition of cytosol reception of estradiol and nuclear acception of estrogen-receptor complexes in target organs of female rats subjected to gamma-radiation was studied. It was established that the action of ionizing radiation of 0.5 and 1.0 Gy dose (dose rate 6.2 sGy per minute) was combined with a decrease (liver, uterus) of cytosol estrogen-receptor complexes and their modification and that those effects were realized through the lessening of the nuclear acception capability in liver cells of radiation-exposed animals.

Acception of estrogen and progestin receptor complexes by hepatocyte nuclei in irradiated female rats.

Ionizing radiation in doses of 0.5 and 1.0 Gy modifies cytosol estrogen- and progestin-receptor complexes decreasing their acception by hepatocyte nuclei in the liver of gamma-irradiated female rats.

Comparative morphofunctional investigation of vascular pool of the muscles of the elongated extremity using different distractional regimens.

Morphofunctional changes in intra-organ vessels of dogs' muscles were evaluated by rheovasography and stereometry methods of muscular preparations to reveal ATP-ase, biochemical methods were used to determine tissue metabolism indices during elongation of tibia using different distractional regimens 1 mm 4 times per day (manual adjustment) and 1 mm 60 times per day (automatic distraction). The data obtained demonstrate different mechanisms for adequate intra-organ blood circulation in muscles using different distractional regimens.

Steroid-receptor complexes and their interaction with the rat liver nuclei during ontogenesis.

Steroid-receptor complexes (SRCs) (glucocorticoids, estrogens and progestins) are isolated from rat liver and purified 1500-2000-fold. Both the initial cytosol complexes and those purified 2000-fold were characterized by gel filtration on Sephadex G-100 and DEAE-cellulose chromatography. The purified rat liver SRCs were used for binding to the isolated liver nuclei from rats of different ages (1.5, 6, 12, 24 months). Binding of progestin and estrogen-receptor complexes from rat liver to homological nuclei of 1.5-month-old rats is maximum, no distinct differences being found in the case of glucocorticoid-receptor complexes. SRC binding to the nuclei progressively declines with age, reaching its minimum by 24 months. The revealed differences in SRC binding by the nuclei of animals of different ages seem to be the basis of the hormone-induced changes in the organism functioning at various stages of ontogenesis.

[Estrogen- and progestin-receptor complexes and their interactions with rat liver cell nuclei in ontogenesis]. / Estrogen- i progestin-retseptornye kompleksy i ikh vzaimodeistvie s iadrami kletok pecheni krys v ontogeneze.

Steroid-receptor complexes (SRC) of estrogen and progestin were isolated from rat liver and purified 1500-2000-fold. The SRC within the composition of cytosol and purified 2000-fold were characterized by gel filtration of Sephadex G-100 and by DEAE-cellulose chromatography. The purified SRC from rat liver were bound to isolated liver cell nuclei of rats of various age (1.5, 6, 12 and 24 month-old). The maximal binding of progestin and estrogen SRC from rat liver was observed in homologous nuclei of 1.5-month-old animals. The binding of SRC by the nuclei decreased progressively with age, reaching its minimum in 24-month-old rats. The observed differences in the SRC binding by cell nuclei of experimental animals may be the cause of functional changes at various stages of ontogenesis.