The Effects of Foods Embedded in Entertainment Media on Children's Food Choices and Food Intake: A Systematic Review and Meta-Analyses.

While watching or playing with media, children are often confronted with food appearances. These food portrayals might be a potential factor that affects a child's dietary behaviors. We aimed to comprehensively expound the effects of these types of food appearances on dietary outcomes of children. Our objectives were to synthetize the evidence of the experiments that study the effects of foods embedded in children's entertainment media throughout a systematic review, to conduct two meta-analyses (food choice and intake) in order to quantify the effects, and to examine to what extent the effects of foods embedded in entertainment media varies across different moderating variables. We conducted a systematic search of five databases for studies published up to July 2018 regarding terms related to children and foods embedded in entertainment media. We identified 26 eligible articles, of which 13 (20 effect sizes) and 7 (13 effect sizes) were considered for a meta-analysis on food choice and intake, respectively. Most of the studies were assessed as having a middle risk of bias. Overall, food being embedded in entertainment media is a strategy that affects the eating behaviors of children. As most of the embedded foods in the included studies had low nutritional values, urgent measures are needed to address the problem of childhood obesity.

Investigating the Effects of Non-Branded Foods Placed in Cartoons on Children's Food Choices through Type of Food, Modality and Age.

Cartoons are among the most consumed media products by children, especially those at a young age. While branded food placements are not allowed in animated series, non-branded food placements are prevalent. However, little is known about the effects that these food placements might have on children's eating preferences. In an experimental study with 124 children (51.6% girls, age range: 7-11, Mage = 9.24, and SD = 1.19), 62 children in the experimental condition were exposed to 16 food placements in cartoons, whereas children in the control condition were exposed to cartoon scenes without foods. The healthiness of the placed foods (low nutritional value foods versus high nutritional value foods) as well as the modality of food placements (unimodal versus bimodal) were manipulated. After watching the cartoon scenes, children completed a choice task where each placed food appeared on a separate choice card. Our results indicate that non-branded low nutritional value foods placed in cartoons are an effective strategy in modifying children's food choices when children are under age 9. We suggest that policy makers, particularly those involved in the content design of cartoons, take these results into account when placing low nutritional value foods in cartoons, especially for an animated series that targets young child audiences.

Non-Branded Food Placements in Children's Entertainment Programs: A Content Analysis.

Food messages transmitted in media are considered to affect children´s eating habits. Depending on the healthiness of the food messages, these food portrayals can be supportive or non-supportive in educational food content, affecting eventually childhood obesity. This study aims to analyze how food references present in children´s animated cartoons are depicted, considering not only their prevalence and prominence but also their educational nature which is measured by the overall message that is being transmitted. A content analysis of non- branded food placements was conducted on the basis of 25 international cartoon series for children (aged 3-12) with 4,790 minutes of viewing. A total of 1,065 food placements occurred with a rate of one placement approximately every five minutes. A balance was found between the frequency of low and highly-recommended consumption foods and the same happened considering the educational nature of the global message. When the target age and the country of origin were considered, the least educational messages predominated in those series aimed at the oldest children group and produced in North America. This study intends to contribute to the problem of childhood obesity by indicating the educational nature of the food messages that children are confronted with. As prevalence and prominence of non-supportive food placements to educational food content is high, policy makers should consider these findings when designing public policies that aim to prevent childhood obesity.

The gene product of a Trypanosoma equiperdum ortholog of the cAMP-dependent protein kinase regulatory subunit is a monomeric protein that is not capable of binding cyclic nucleotides.

The full gene sequence encoding for the Trypanosoma equiperdum ortholog of the cAMP-dependent protein kinase (PKA) regulatory (R) subunits was cloned. A poly-His tagged construct was generated [TeqR-like(His)8], and the protein was expressed in bacteria and purified to homogeneity. The size of the purified TeqR-like(His)8 was determined to be ∼57,000 Da by molecular exclusion chromatography indicating that the parasite protein is a monomer. Limited proteolysis with various proteases showed that the T. equiperdum R-like protein possesses a hinge region very susceptible to proteolysis. The recombinant TeqR-like(His)8 did not bind either [3H] cAMP or [3H] cGMP up to concentrations of 0.40 and 0.65 µM, respectively, and neither the parasite protein nor its proteolytically generated carboxy-terminal large fragments were capable of binding to a cAMP-Sepharose affinity column. Bioinformatics analyses predicted that the carboxy-terminal region of the trypanosomal R-like protein appears to fold similarly to the analogous region of all known PKA R subunits. However, the protein amino-terminal portion seems to be unrelated and shows homology with proteins that contained Leu-rich repeats, a folding motif that is particularly appropriate for protein-protein interactions. In addition, the three-dimensional structure of the T. equiperdum protein was modeled using the crystal structure of the bovine PKA RIα subunit as template. Molecular docking experiments predicted critical changes in the environment of the two putative nucleotide binding clefts of the parasite protein, and the resulting binding energy differences support the lack of cyclic nucleotide binding in the trypanosomal R-like protein.