Ultrasound and fine needle aspiration cytology of the axilla in the pre-operative identification of axillary nodal involvement in breast cancer.

AIMS: Pre-operative diagnosis of axillary nodal involvement in breast cancer allows one-stage axillary surgery. We evaluated the efficacy of axillary ultrasound (US) with US guided fine needle aspiration cytology (FNAC) in the diagnosis of axillary nodal involvement. METHODS: Over a 13-month period, we performed US of 369 axillae in patients with screen-detected (n = 278) and symptomatic (n = 91) invasive carcinoma of the breast, at the same time as US of the primary tumour. If abnormal lymph nodes were demonstrated, a single US guided FNAC of the most abnormal node was performed. US and FNAC results were compared with the final histology of the surgically excised lymph nodes. RESULTS: Among the 369 axillae studied, 102 had nodal macrometastases and 38 (37%) were identified by US guided FNAC. The rate was 33% in screen-detected and 44% in symptomatic patients. Sensitivity increased with increasing numbers of positive axillary nodes, and the more abnormal the appearances of the nodes on US. CONCLUSION: US with FNAC of the most abnormal node allows pre-operative detection of a third of node positive axillae in screen-detected and over 40% of those with symptomatic breast cancer, allowing one-stage axillary surgery avoiding the sentinel node biopsy step in these patients.

The use of carbon suspension as an adjunct to wire localisation of impalpable breast lesions.

AIM: To determine the accuracy and therapeutic success of localisation of impalpable breast lesions by hookwire with additional lesion marking with carbon suspension to mark screen detected abnormalities requiring surgical excision. MATERIALS AND METHODS: Retrospective review of all breast localisation procedures performed in our unit on women with a screen detected abnormality requiring excision over a 7 year period. RESULTS: One hundred and thirty eight women underwent breast localisation procedures. All of the mammographic abnormalities were excised at the initial surgical procedure. The benign to malignant ratio was 1:2. Pre-operative cytology was used to guide the extent of surgical excision, with clear margins in 70 of the 92 patients (75 percent) with malignancy. Twenty patients had further surgery: mastectomy in 7 and further local excision in 14. The localisation procedure was a therapeutic success in the local excision of malignancy in 73 of the 92 patients (79 percent) with malignancy. CONCLUSION: This method of localisation biopsy is an accurate technique for surgical excision of mammographically detected impalpable abnormalities. The surgeon is able to choose the site of surgical incision to give the best cosmetic result, the lesion is easier to identify at operation and the confidence that the abnormality has been excised is improved.

On the origin of the kattunayakas of nilgiris.

This paper presents an account of the origin of Kattunayakas , one of the tribes of the Nilgiris, Tamil Nadu.

Discordance between cytologic and histologic reports in cervical intraepithelial neoplasia. Results of a one-year audit.

In order to investigate the factors contributing to cases in which the cytology and histology reports of cervical intraepithelial neoplasia (CIN) differ, we assessed the impact of careful review of the biopsy and its corresponding cervical smear. In a one-year audit of all cervical biopsies we found that 18.8% of biopsy-smear pairs disagreed by at least two grades of CIN. Following review the mismatch rate fell by 47%, mainly due to a drop in the number of cases in which the smear showed less severe CIN than did the biopsy. The proportion of cases in which the cytologic impression of CIN was greater than the histologic was changed little. The fall in the mismatch rate was seen after review of the smears, while a similar review of the histology did not alter the rate of mismatch. Neither the presence of koilocytotic changes on either cytology, histology or both, nor the size of the biopsy (punch vs. cone/hysterectomy) influenced the occurrence of such discrepancies. A similar review of the smears and biopsies of matching cases of CIN revealed no significant changes. This suggests particular difficulties of interpretation in the mismatching cases. In those cases with persistent mismatch an additional element of sampling error must be assumed to be the main cause even though all smears were considered of adequate quality.

An analysis of the variation of human interpretation: Papnet a mini-challenge.

The Papnet was given a mini-challenge of 200 cervical smears loaded to 50% with varying degrees of abnormality as interpreted by the originating laboratory. The range of abnormality extended from 'atypia' to invasive cancer and a few 'glandular' lesions were included as were a few smears which had been reported as 'inadequate'. Three cytologists (two cytopathologists and one cytotechnologist) read and analysed the 128 monitor pictures per slide, selected by the Scanning Algorithm and Neural Network systems. These results were compared with a 'gold standard' report on the glass slide produced by two cytopathologists. The analysis was done for each individual cytologist, for cases in which all three agreed, for a consensus between two of the three and for the 'best of three'. The latter gave an error rate of 4% false negative (Papnet scan negative) and 10% 'false positive' (referred for glass slide examination). Individual cytologists had higher error rates demonstrating that errors could be due to human interaction and not necessarily to the Scanner. This also indicated that wide experience in interpretation of monitor images is needed to achieve high quality results.

What is vestibular papillomatosis? A study of its prevalence, aetiology and natural history.

OBJECTIVES: To clarify the prevalence, aetiology, symptoms and natural history of vestibular papillomatosis. DESIGN: Study in two parts: 1. prevalence assessed by colposcopic examination of the vulva of unselected patients by one doctor (J.M.W.); 2. patients selected by clinical appearance as having vestibular papillomatosis (by J.M.W. and other doctors) assessed in a research clinic. SETTING: Outpatient genitourinary medicine clinic in South London. SUBJECTS: Part 1 study: 295 female clinic attenders; part 2 study: 18 women with clinical vestibular papillomatosis. MAIN OUTCOME MEASURES: Part 1 study: number of unselected patients found to have vestibular papillomatosis. Part 2 study: associated symptoms, histology, DNA hybridisation and polymerase chain reaction on vulval biopsies. Clinical regression of lesions. Cervical cytology and colposcopy. RESULTS: Part 1 study: Vestibular papillomatosis was identified by colposcopic examination of the vulva in 3/295 (1%) of women. Part 2 study: 9/18 (50%) women with vestibular papillomatosis were asymptomatic; the other nine had intermittent mild symptoms. Thirteen (72%) had a history of genital warts. Vulval biopsies had features suggestive of wart virus infection on histology in 17/18 (94%) and HPV16 was found by DNA hybridisation studies or polymerase chain reaction in 7/18 (39%). On follow up (mean duration 9 months) the vulval lesions had regressed in 9/12 patients. Ten patients had cervical wart virus infection or intraepithelial neoplasia (CIN), or both, and five needed laser treatment for this. CONCLUSIONS: In this study vestibular papillomatosis was associated with human papillomavirus (HPV) infection. This study suggests that vestibular papillomatosis need not be treated, but patients with it may be at increased risk for CIN.

Less known edible fruit - yielding plants of nilgiris.

The present paper is concerned with 27 species belonging to 22 generate and 18 families, which yield wild edible fruits. They are arranged in alphabetical order followed by their local names and habit. An attempt has been also made to indicate the nutritive values of edible portions on the basis of documented literature. Brief illustration is furnished wherever necessary.

The association between sexually transmitted diseases and inflammatory cervical cytology.

OBJECTIVES: To assess the significance of inflammatory changes as a marker of sexually transmitted diseases and their use as a diagnostic aid. METHODS: 363 patients attending a department of genito urinary medicine were examined prospectively. All underwent cervical cytology and full STD screening. Cervical cytology was assessed for evidence of the presence or absence of inflammatory changes. RESULTS: There was no significant association between inflammatory cytology and cervical ectropion or dyskaryosis. Infections with Chlamydia trachomatis and Trichomonas vaginalis were significantly associated with inflammatory changes but there was no significant association with chlamydia alone, and 91.1% of T. vaginalis infections were detected on cytology. CONCLUSION: The presence of inflammatory changes on cervical cytology seems a poor indicator of sexually transmitted diseases.

Histological differences between colposcopic-directed biopsy and loop excision of the transformation zone (LETZ): a cause for concern.

Colposcopic examination and biopsy were performed on 100 patients with abnormal cervical cytology. All were suitable for ablative therapy but were treated by loop excision of the transformation zone (LETZ) as outpatients. Histological comparison between the colposcopic biopsies and LETZ samples showed poor agreement with only 43% of cases illustrating identical degrees of dysplasia. The dysplasia was underestimated by colposcopic biopsy in 16% of cases and overestimated in 41% of cases, when compared to the LETZ specimen. Microinvasive carcinoma was diagnosed in 3 cases on the LETZ histology, having been missed by the preceding colposcopy and colposcopic biopsy. The disparity between histological findings is of great concern and would not have been detected if the patients were treated by ablative therapy. It is the authors' view that LETZ should replace ablative therapy in the treatment of localized cervical dysplasia and that it may avoid the need for pretreatment colposcopic biopsy. LETZ limits the possibility of inadequate treatment and also provides a method of audit for the colposcopist.

Non-invasive management of fever and breathlessness in HIV positive patients.

In a prospective study of 72 human immunodeficiency virus (HIV) positive patients presenting with fever and breathlessness, a non-invasive management protocol, incorporating a scanning technique using radioactively labelled diethylenetriamine penta acetate (DTPA) and sputum induction, was found to be highly sensitive and specific in the early detection of Pneumocystis carinii pneumonia (PCP). At presentation, the DTPA scan was abnormal in 34 of 36 cases of PCP, irrespective of smoking history, whilst the chest radiograph was diffusely abnormal in 21 cases. Sputum induction identified 7 of 14 patients with PCP in the first six months of its use and 7 of 10 patients over the last six months. The DTPA lung scan and induced sputum examination are non-invasive techniques which can be used to investigate out-patients. In combination they detected all cases of PCP at presentation, reduced the need for bronchoscopy, resulted in a low case fatality (5.4%) and reduced the need for admission.

Examination of induced sputum in the diagnosis of Pneumocystis carinii pneumonia.

The results of the examination of sputum induced by the inhalation of nebulized hypertonic saline in the diagnosis of Pneumocystis carinii pneumonia (PCP) are presented. In suspected cases of PCP in patients who were either HIV antibody positive or were receiving immunosuppressive therapy, 46 induced sputum specimens were stained using both Grocott's modified Gomori methenamine silver nitrate (GMS) and immunofluorescence staining. In 12 specimens P. carinii cysts were detected by both methods, in four specimens by GMS staining only and in five specimens by immunofluorescence only. The sensitivity of induced sputum examination in the detection of P. carinii cysts was increased by using both of these staining methods on each sputum specimen and the need for more invasive methods of diagnosis was reduced.

A comparison of four cytological sampling techniques in a genitourinary medicine clinic.

Four cytological sampling techniques (the Ayre's spatula alone, the Ayelesbury spatula, the Ayre's spatula in conjunction with a Cytobrush (Medscand), and the Cervex (Steriseal] were compared when used in a genitourinary medicine clinic. Over a period of two years 6991 smears were taken. No difference was found between the methods with regard to detection of dyskaryosis, although there were significant differences in the percentage of smears containing endocervical cells.

Fine needle aspiration cytology of salivary gland lesions: advantages and pitfalls.

Fine needle aspiration cytology (FNAC) was performed on 52 patients with salivary gland lesions. A definitive cytodiagnosis was possible in 45 cases: a sensitivity of 89% and a specificity of 94% was achieved. The pitfalls of FNAC of salivary gland lesions are reflected by the false positive and false negative rates which were 4%. Errors of cytodiagnosis are due to the morphological variability of these tumours which make sampling and interpretation difficult.