Formulation of Edible Coatings from Alfalfa Saponins to Enhance the Postharvest Quality of Tomatoes.

Tomatoes are a major crop for global exports and have significant nutritional benefits. However, their lifespan is limited due to various biotic and abiotic factors. This study aimed to formulate an edible coating using crude alfalfa saponins coupled with decaglycerol monolaurate (ML-750) and polyoxyethylene (20) sorbitan monolaurate (Tween 20), to enhance the postharvest quality and shelf life of tomatoes by preventing spoilage. The effectiveness of alfalfa saponins coatings, both alone, and with ML-750 and Tween 20, was evaluated by comparing their impact on color, texture, overall acceptability, and % weight loss at 4°C and 25°C for 7 days. Significant improvements were observed in the quality attributes of tomatoes, including firmness, aroma, color, texture, and overall acceptability. Crude alfalfa saponins in emulsified form with Tween 20 increased the shelf stability of tomatoes more effectively than uncoated and ML-750 combined coatings. The total soluble solids (TSS) and pH also play a crucial role in determining the quality of the fruits. The results indicated no significant changes in the TSS of tomatoes coated with encapsulated saponins. Subsequently, a gradual increase in the pH of the coated tomatoes was observed on days 5 and 7, respectively. The findings of this study revealed that alfalfa saponins coupled with synthetic emulsifiers may be a beneficial strategy for prolonging the shelf life and improving the postharvest quality of tomatoes.

A comprehensive review on chlorpyrifos toxicity with special reference to endocrine disruption: Evidence of mechanisms, exposures and mitigation strategies.

Chlorpyrifos (CPF) is a broad-spectrum chlorinated organophosphate (OP) pesticide used for the control of a variety of insects and pathogens in crops, fruits, vegetables, as well as households, and various other locations. The toxicity of CPF has been associated with neurological dysfunctions, endocrine disruption, and cardiovascular diseases (CVDs). It can also induce developmental and behavioral anomalies, hematological malignancies, genotoxicity, histopathological aberrations, immunotoxicity, and oxidative stress as evidenced by animal modeling. Moreover, eye irritation and dermatological defects are also reported due to CPF toxicity. The mechanism of action of CPF involves blocking the active sites of the enzyme, acetylcholinesterase (AChE), thereby producing adverse nervous system effects. Although CPF has low persistence in the body, its active metabolites, 3,5,6-trichloro-2-pyridinol (TCP), and chlorpyrifos-oxon (CPO) are comparatively more persistent, albeit equally toxic, and thus produce serious health complications. The present review has been compiled taking into account the work related to CPF toxicity and provides a brief compilation of CPF-induced defects in animals and humans, emphasizing the abnormalities leading to endocrine disruption, neurotoxicity, reproductive carcinogenesis, and disruptive mammary gland functionality. Moreover, the clinical signs and symptoms associated with the CPF exposure along with the possible pharmacological treatment are reported in this treatise. Additionally, the effect of food processing methods in reducing CPF residues from different agricultural commodities and dietary interventions to curtail the toxicity of CPF has also been discussed.

Formulation and characterization of oil-in-water nanoemulsions stabilized by crude saponins isolated from onion skin waste.

In the current work, a detailed study of crude saponins isolated from onion skin waste (OSW) was carried out to minimize the interfacial tension (γ) at oil-water interfaces, and to evaluate important factors on the formulation and characterization of nanoemulsions (NEs) stabilized by OSW saponins as a new natural emulsifier. It was found that OSW saponins are moderately strong surface-active agents. The droplet size of OSW saponin-stabilized NEs formulated by a high-pressure homogenizer was significantly dependent on processing parameters, where volume mean droplet diameter (d 4,3) decreased as homogenization pressure and emulsifier concentration increased. The d 4,3 of NEs formulated from soybean oil, sunflower oil, and medium-chain triglyceride (MCT) oil was <140 nm, which was much smaller than those prepared using orange oil (d 4,3 > 800 nm). The OSW saponin-coated droplets in soybean-based NEs showed stability against thermal treatment (30-90 °C, 30 min), pH (7-9), and ionic strength (NaCl < 50 mM). The NEs exhibited instability with droplet coalescence at relatively low pH (3-6), and high ionic strength (NaCl > 50 mM). The NEs stabilized by 0.5-1% (w/w) OSW were highly stable without an increase in d 4,3 during the storage (60 days) at 25 °C. Overall, these results suggest that OSW saponins are an efficient natural emulsifier, with significant potential to replace synthetic emulsifiers in certain practical applications.