Recycling anaerobic digestate enhances the co-digestion potential of agro-industrial residues: influence of different digestates as sources of microbial inoculum.

Anaerobic codigestion (AcD) of agroindustrial residues was investigated. Granular sludge from bench-scale bioreactors digesting different manure were acclimated and recycled as microbial seed sludge to demonstrate inoculum-type influence on digestion performance. The biomethane potential (BMP) assay was operated for 30 days at 40 ± 2 °C in batch-type laboratory-scale reactors (100 mL). In inoculum amended reactors, codigestion showed significant, yet distinctive, biomethanation than monodigestion with a 5-fold increase (p < 0.05) in average biogas (248.3 ± 5.30 mL gVS-1) and CH4 yield (207.5 ± 4.15 mL gVS-1). The pH, soluble chemical oxygen demand (sCOD) and volatile fatty acids (VFAs) concentrations were within limits for stable AcD process with elevated total solids (TS) and volatile solids (VS) removal efficiencies. This study reinforces advancements in the recycling of digestate in biodigesters and suggests the appropriate selection of inoculum, preferably cow manure, to essentially boost methane production from these wastes.

Anaerobic co-digestion of spent coconut copra with cow urine for enhanced biogas production.

Laboratory-scale bioreactors were used to co-digest spent coconut copra (SCC) and cow urine (CU) as a co-substrate (SCC + CU) in a batch mode under thermophilic condition (45 ± 2°C) in order to enhance biogas production. The effect of CU pretreatment on the performance indicators (biogas and biomethane yields, total solids (TS), and volatile solids (VS) reduction, pH and volatile fatty acids (VFAs) concentrations) were also examined. This was compared with mono-digestion of SCC. The experiment was performed with different mixing ratios in reactors labelled as follows: A = 75 g SCC + 5 ml CU; B = 70 g SCC + 10 ml CU; C = 65 g SCC + 15 ml CU; and D (control) = 80 g SCC at a hydraulic retention time of 42 days. Co-digestion (SCC + CU) significantly improved anaerobic digestion (AD) performance resulting in a threefold and fivefold increase in biogas and biomethane production, respectively, with concomitant TS (44.9-57.7%) and VS (55.4-60.3%) removal efficiencies. But for mono-digestion (control experiment), all CU treated and co-digestion assays showed pH stability ranging between 6.6 and 7.4 and VFAs' concentrations ranging from 15-330 mgL-1. By acting as a buffer, CU effectively enhanced the AD performance of SCC as demonstrated in this study.