[Hemoglobin polymers during their circulation in the vascular bed of animals]. / Izuchenie polimerov gemoglobina v protsesse ikh tsirkuliatsii v krovenosnom rusle zhivotnykh.

The effect of molecular mass of 1 g/kg hemoglobin (Hb) modified by glutaraldehyde and pyridoxal-5'-phosphate on the duration of Hb presence in the rabbit circulation was studied. Hb concentration and met-form content were measured in plasma samples taken 5 min, 6, 18 and 24 h after the injection. Gel and ion exchange chromatography was performed. Predominant clearance of low-molecular fractions within the first 6 h of circulation was shown, it was followed by a rise in high-molecular fraction (greater than 300,000) content, that might be caused by the interaction of modified Hb with plasma proteins. This corroborates the decrease in the overall charge of the circulating fractions.

[Biotesting of a burn toxin on mice with reticuloendothelial system block]. / Biotestirovanie ozhogovogo toksina na myshakh s blokadoi retikuloéndotelial'noi sistemy.

Experiments on mice with reticuloendothelial system blockade were made to assay the toxic properties of the preparations obtained from the skin of burn and normal rats. As regards the activity level the preparations can be classified with either highly toxic or little toxic. Analysis of the dose-response dependence indicates that toxicity of the extracts is accounted for by at least two different toxins.

[Therapeutic effectiveness of gamma-globulin on experimental burns]. / Lechebnaia éffektivnost'gamma-globulina na éksperimental'nykh ozhogakh.

Experiments on rabbits were made to examine excretory-absorption liver function and the blood kallikrein-kinin system after thermal burn and treatment with gamma-globulin immune to burn skin toxin. Immunotherapy leads to a more rapid and effective recovery of the functional indicators in the burnt animals than in burns without or with the treatment by nonimmune preparations. It was demonstrated that specific detoxification plays an important role in multiple modality treatment of burn disease.

[Action of a high-molecular toxin from burned skin on the hepatocyte lysosomal apparatus]. / O deistvii vysokomolekuliarnogo toksina obozhzhennoi kozhi na lizosomal'nyi apparat gepatotsitov.

Experiments on rats were made to study the action of toxic glycoprotein isolated from burn skin on the lysosomal apparatus of hepatocytes and morphological structure of the liver. It was shown that 2-2.5 h after intraperitoneal injection to normal rats of toxic preparations of varying purity, the animals developed morphological alterations similar to those seen in the liver of the burn animals. At the same time there was an appreciable increase in the percentage of unsedimentable activity of catepsine D of the total one, thereby indicating the toxin-induced injury to the lysosomal apparatus of hepatocytes.

[Phagocytic activity of the reticuloendothelial system in burn toxicity]. / Fagotsitarnaia aktivnost' retikuloéndotelial'noi sistemy pri ozhogovoi intoksikatsii.

The functional activity of the reticuloendothelial system (RES) was studied in 85 Wistar rats with burn intoxication induced by thermal injury or administration of burn toxoid. It was established that burn injury causes a stable and marked suppression of RES phagocytic activity. This was manifested by a rise in the percentage of Au radioactivity retention in the blood flow and by the decreased phagocytic index. Injections of burnt skin extracts and burn toxoid preparations, i. e. stimulation of burn intoxication depresses the phagocytic activity of hepatic macrophages similarly to the action of natural burn. A decrease in the phagocytic activity of hepatic macrophages under the effect of burn intoxication may serve as a sensitive indicator of the body nonspecific resistance in burn injury, whereas burn toxoid may be regarded as a initiator of changes in the early stage of burn disease.

[Toxicity of burned skin high molecular weight protein according to the results of biological experiments in vivo and in vitro]. / Toksichnost' vysokomolekuliarnogo belka obozhzhennoi kozhi po rezul'tatam bioispytanii in vivo i in vitro.

In vivo and in vitro experiments have demonstrated the toxic effect of high-molecular protein isolated from burnt skin extract (BSE) by immunosorption. The toxin preparations cause the death of mice with RES blockade and inhibit the leukocyte migration capacity in culture. It was shown in control experiments that BSE after passage through the immunosorbent fully loses its toxic properties because of toxin binding to insoluble-base-fixed antibodies.

[Functional state of the liver following massive transfusion of packed red cells]. / Funktsional'noe sostoianie pecheni posle massivnykh transfuzii éritrotsitnoi massy.

Hepatic function was studied in experiments on 16 dogs after massive transfusion of red cell pack to ascertain the blood components influencing this function most. Massive transfusion of red cell pack failed to induce any significant changes in the excretory-absorbing protein forming function, and also in the content of transaminases. Hepatic function was markedly disturbed in a control group given massive transfusions of whole homologous blood. Results of investigations support the suggestion that one of the causes of hepatic function disturbances in "the syndrome of massive blood transfusion" was donor's and recipient's plasma protein incompatibility.