[The development of a C1q-solid-phase immunoenzyme method for determining circulating immune complexes]. / Razrabotka C1q-tverdofaznogo immunofermentnogo metoda opredeleniia tsirkuliruiushchikh immunnykh kompleksov.

The method of quantitative enzyme immunoassay (EIA) for the determination of circulating immune complexes (CIC) was developed on the basis of solid-phase human C1q. The calibration curve was plotted with the use of aggregated human gamma-globulin (AHGG), the optimum range of concentration being 15-500 microg/ml. In the process of approbation on clinical material the method revealed an elevated level of CIC in the sera of patients in comparison with their level in the sera of healthy donors. Out of 40 studied serum samples from patients with Yersinia infection, in 3 serum samples the levels of CIC was 26, 65 and 94 microg of AHGG equivalents per ml. In 4 out of 46 studied serum samples obtained from patients with diagnosed Yersinia arthritis the level of CIC was 12, 27, 46 and 186 microg of AHGG per ml, and in serum samples from healthy donors this level was 8.6 microg/ml [corrected].

[Effect of gamma radiation on the immunobiological and immunochemical properties of cholera exotoxin. IV. The biological and immunochemical properties of purified irradiated choleragen]. / Vliianie gamma-izlucheniia na immunobiologicheskie i immunokhimi-cheskie svoistva kholernogo ékzotoksina. Soobshchenie IV. Biologicheskie i immunokhimicheskie svoistva ochishchennogo obluchennogo kholerogena.

The results of investigations carried out to study the effect of gamma radiation on the properties of the purified preparations of cholera exotoxin are presented. Irradiation has been shown to decrease the anterotoxicity of purified choleragen and the activity of its permeability factor, depending on the radiation dose. The investigations have revealed that in purified toxin enterotoxicity is completely inactivated with a lover radiation dose than in crude toxin filtrate (25 kGy). In immunochemical reactions the increase of the electrophoretic mobility of the choleragen components, correlated with the increase of the radiation dose, and the reduced number of protein zones have been observed. The irradiated preparations of purified choleragen have been found to retain their immunogenic properties and serological activity.

[Effect of gamma radiation on the immunobiological and immunochemical properties of cholera exotoxin. III. The serological activity and immunochemical properties of irradiated unpurified toxin]. / Vliianie gamma-izlucheniia na immunobiologicheskie i immunokhimicheskie svoistva kholernogo ékzotoksina. Soobshchenie III. Serologicheskaia aktivnost' i immunokhimicheskie svoistva obluchennogo neochischennogo toksina.

The immunochemical properties and serological activity of irradiated preparations of crude cholera exotoxin have been studied. This study has revealed that with the increase of the dose of ionizing radiation changes occur in the physico-chemical properties of the preparations of the toxin, which leads to an increase in the electrophoretic motility of the protein components of the toxin, to the aggregation and polymerization of individual fragments. The preparations of antigen exotoxins have been shown to retain their serological activity within the range of radiation doses under study (10-350 kGy).

[Effect of gamma radiation on the immunobiological and immunochemical properties of cholera exotoxin. I. Change in the biological activity of nonpurified cholera exotoxin as affected by ionizing radiation]. / Vliianie gamma-izlucheniia na immunobiologicheskie i immunokhimicheskie svoistva kholernogo ékzotoksina. Soobshchenie I. Izmenenie biologicheskoi aktivnosti neochishchennogo kholernogo ékzotoksina pod vozdeistviem ioniziruiushchei radiatsii.

Crude cholera exotoxin (filtrate toxin) was irradiated with increasing doses of gamma radiation. A significant drop in enterotoxicity, in the activity of the permeation factor and a decrease in toxicity were shown to occur as radiation doses increased. Radiation doses of 50-70 kGy were found to completely inactivate enterotoxicity in liquid toxic preparations. A higher radioresistance of dried preparations in comparison with liquid ones was registered: inactivation occurred at 150-200 kGy. Different batches of the initial filtrate toxin had varying radiosensitivity. The sterilizing effect of gamma radiation was achieved at doses of 20 kGy for liquid preparations and 30 kGy for dried preparations. During the prolonged storage of the irradiated preparations of crude toxin (the term of observation being 1.5 years) at different temperatures no reversion of toxicity was found to occur, while their immunogenic properties remained unchanged.