Can the Seed Trade Provide a Potential Pathway for the Global Distribution of Foliar Pathogens? An Investigation into the Use of Heat Treatments to Reduce Risk of Dothistroma septosporum Transmission via Seed Stock.

The international plant trade results in the accidental movement of invasive pests and pathogens, and has contributed significantly to recent range expansion of pathogens including Dothistroma septosporum. Seeds are usually thought to present a lower biosecurity risk than plants, but the importation of Pinus contorta seeds from North America to Britain in the mid-1900s, and similarities between British and Canadian D. septosporum populations suggests seeds could be a pathway. Dothistroma septosporum has not been isolated from seeds, but inadequately cleaned seed material could contain infected needle fragments. This case study investigated whether cone kilning, and wet and dry heat treatments could reduce D. septosporum transmission without damaging seed viability. Pinus needles infected with D. septosporum were incubated alongside cones undergoing three commercial seed extraction processes. Additional needles were exposed to temperatures ranging from 10 to 67 °C dry heat for up to 48 h, or incubated in water heated to between 20 and 60 °C for up to one hour. Pinus sylvestris seeds were exposed to 60 and 65 dry heat °C for 48 h, and further seed samples incubated in water heated to between 20 and 60 °C for up to one hour. Dothistroma septosporum survived the three kilning processes and while seeds were not damaged by dry heat exceeding 63.5 °C, at this temperature no D. septosporum survived. Wet heat treatments resulted in less than 10% pathogen survival following incubation at 40 °C, while at this temperature the seeds suffered no significant impacts, even when submerged for one hour. Thus, commercial seed kilning could allow D. septosporum transmission, but elevated wet and dry heat treatments could be applied to seed stock to minimise pathogen risk without significantly damaging seed viability.

Non-Invasive Assessment of Back Surface Topography: Technologies, Techniques and Clinical Utility.

(1) Background: Frequent exposure to ionising radiation is often used to determine the diagnosis of adolescent idiopathic scoliosis (AIS), a lateral curvature of the spine in those aged between 10 and 18 years, and a treatment plan according to Cobb angle. This narrative review outlines the clinical utility of surface topography (ST), a radiation-free imaging modality. (2) Methods: Publicly available databases were searched to yield literature related to ST. Identified articles were classified based on the equipment used and in order of how it was developed, i.e., historical, recent developments, and state-of-the-art developments. (3) Conclusions: ST is a reliable cost-effective non-invasive technique that provides an alternative to radiation-based imaging to aid with the diagnosis and potential screening of AIS. Several scanning methods are available, which allows ST to be used in several clinical environments. Limitations of inter-reliability and differences of apparatus resulting in variations of data have been noted through this narrative review.

The Kinematics of Fixed-Seat Rowing: A Structured Synthesis.

Olympic-style sliding-seat rowing is a sport that has been extensively researched, with studies investigating aspects related to the physiology, biomechanics, kinematics, and the performance of rowers. In contrast, studies on the more classic form of fixed-seat rowing are sparse. The aim of this study is to address this lacuna by analysing for the first time the specific kinematics of fixed-seat rowing as practised by able-bodied athletes, thus (i) documenting how this technique is performed in a manner that is replicable by others and (ii) showing how this technique compares and contrasts with the more standard sliding-seat technique. Fixed-seat rowing was replicated in a biomechanics laboratory where experienced fixed-seat rowers, marked with reflective markers following the modified Helen-Hayes model, were asked to row in a manner that mimics rowing on a fixed-seat boat. The findings from this study, complimented with data gathered through the observation of athletes rowing on water, were compared to sliding-seat ergometer rowing and other control experiments. The results show that, in fixed-seat rowing, there is more forward and backward thoracic movement than in sliding-seat rowing (75-77° vs. 44-52°, p < 0.0005). Tilting of the upper body stems was noted to result from rotations around the pelvis, as in sliding-seat rowing, rather than from spinal movements. The results also confirmed knee flexion in fixed-seat rowing with a range of motion of 30-35°. This is less pronounced than in standard-seat rowing, but not insignificant. These findings provide a biomechanical explanation as to why fixed-seat rowers do not have an increased risk of back injuries when compared with their sliding-seat counterparts. They also provide athletes, coaches, and related personnel with precise and detailed information of how fixed-seat rowing is performed so that they may formulate better and more specific evidence-based training programs to meliorate technique and performance.

Eurasian beavers in Central Italy: perceptions in the local community.

According to the IUCN guidelines, wildlife reintroduction should consider any impacts on humans within feasibility assessments. Eurasian beavers Castor fiber are recovering across their native range, due to protection laws and reintroductions. In Central Italy, a self-sustaining, naturalised population of Eurasian beavers has been identified in the last five years. A questionnaire to measure whether and how citizens in the local area perceive the presence of the beaver was administered to 1114 respondents. We observed a comprehensive awareness of the presence of the beaver in Italy and a high ability to distinguish it from non-native coypus Myocastor coypus (92.3%). We also recorded a general high knowledge of issues related to the presence of the beaver (i.e., potential effects on indigenous biodiversity). The majority (65.5%) of the surveyed population was in favour of reintroducing the beaver in Central Italy, and only 1.2% was firmly against it. The majority of interviewed people was against the removal of beavers from Central Italy (65.8%), whereas only 3.7% was in favor, citing fears of perceived impacts on the river, crops, and fish populations.

On the Kinematics of the Forward-Facing Venetian-Style Rowing Technique.

This work presents a qualitative and quantitative pilot study which explores the kinematics of Venetian style forward-facing standing rowing as practised by able-bodied competitive athletes. The technique, made famous by the gondoliers, was replicated in a biomechanics laboratory by a cohort of four experienced rowers who compete in this style at National Level events in Malta. Athletes were marked with reflective markers following the modified Helen Hayes model and asked to row in a manner which mimics their on-water practise and recorded using a Vicon optoelectronic motion capture system. Data collected were compared to its equivalent using a standard sliding-seat ergometer as well as data collated from observations of athletes rowing on water, thus permitting the documentation of the manner of how this technique is performed. It was shown that this rowing style is characterised by rather asymmetric and complex kinematics, particularly upper-body movements which provides the athlete with a total-body workout involving all major muscle groups working either isometrically, to provide stability, or actively.

Exercise-induced bronchoconstriction in university field hockey athletes: Prevalence, sex differences, and associations with dyspnea symptoms.

Introduction: Exercise-induced bronchoconstriction (EIB) is a prevalent condition in athletes. EIB screening studies identify many athletes with undiagnosed EIB. Moreover, there is a poor relationship between EIB and dyspnea symptoms recalled from memory. Purpose: This study investigated: (I) the prevalence of EIB in British university field hockey athletes; (II) the effect of sex and diagnostic criteria on EIB prevalence; and (III) the association between EIB and contemporaneous dyspnea symptoms. Methods: 52 field hockey athletes (age: 20 ± 2 years; height: 173 ± 9 cm; body mass: 72 ± 10 kg; male = 31; female = 22) completed a eucapnic voluntary hyperpnea (EVH) test with multi-dimensional dyspnea scores measured 3-10 mins post-EVH. A test was deemed positive (EIB+) if a fall index (FI) ≥10% in FEV1 occurred at two consecutive time points post-test (FIATS). Two further criteria were used to assess the effect of diagnostic criteria on prevalence: FI≥10%, determined by a pre-to-post-EVH fall in FEV1 of ≥10% at any single time-point; and FI≥10%-NORM calculated as FI≥10% but with the fall in FEV1 normalized to the mean ventilation achieved during EVH. Results: EIB prevalence was 19% and greater in males (30%) than females (5%). In EIB+ athletes, 66% did not have a previous diagnosis of EIB or asthma and were untreated. Prevalence was significantly influenced by diagnostic criteria (P = 0.002) ranging from 19% (FIATS) to 38% (FI≥10%-NORM). Dyspnea symptoms were higher in EIB+ athletes (P ≤ 0.031), produced significant area under the curve for receive operator characteristics (AUC ≥ 0.778, P ≤ 0.011) and had high negative prediction values (≥96%). Conclusion: Overall, 19% of university field hockey athletes had EIB, and most were previously undiagnosed and untreated. EVH test diagnostic criteria significantly influences prevalence rates, thus future studies should adopt the ATS criteria (FIATS). Contemporaneous dyspnea symptoms were associated with bronchoconstriction and had high negative prediction values. Therefore, contemporaneous dyspnea scores may provide a useful tool in excluding a diagnosis of EIB.

Spine and pelvis coordination variability in rowers with and without chronic low back pain during rowing.

The aim of this study was to compare the spine-pelvis coordination and coordination variability (CV) during rowing in elite rowers with and without chronic low back pain (CLBP). Fourteen professional rowers (6 healthy and 8 with CLBP) participated in this study. 3D kinematic of upper trunk (UT), lower trunk (LT), lower back (LB), and pelvis segments during ergometer rowing at 70% and 100% of peak power were captured. The adjacent segments' coordination and CV were calculated using modified vector coding method. The results showed that segments' range of motion increased in both groups with increasing intensity, especially in CLBP rowers. CLBP rowers showed significantly lower: LT dominancy in LT/LB coordination at both intensities; anti-phase pattern in LB/Pelvis coordination at 100% intensity; UT/LT CV in early recovery, and significantly higher LB/Pelvis CV in final recovery and catch position (p < 0.05). Moreover, both groups showed significantly lower UT dominancy for UT/LT coordination in sagittal plane; higher anti-phase pattern in frontal plane; lower UT/LT CV in sagittal plane, lower LT/LB CV in sagittal and transverse plane, lower LB/Pelvis CV in frontal plane in trunk preparation phase, and a lower UT/LT CV in frontal plane for acceleration phase at 100% versus 70% intensity. In conclusion rowers with CLBP cannot adapt their coordination pattern and its variability with increase in intensity, and the movement in the kinematic chain from pelvis to UT stops in spine-pelvic junction. These findings have practical implications in designing coaching and rehabilitation strategies to facilitate performance and prevent injuries.

Effects of Prior Voluntary Hyperventilation on the 3-min All-Out Cycling Test in Men.

INTRODUCTION: The ergogenic effects of respiratory alkalosis induced by prior voluntary hyperventilation (VH) are controversial. This study examined the effects of prior VH on derived parameters from the 3-min all-out cycling test (3MT). METHODS: Eleven men ( = 46 ± 8 mL·kg-1·min-1) performed a 3MT preceded by 15 min of rest (CONT) or VH ( = 38 ± 5 L·min-1) with PETCO2 reduced to 21 ± 1 mm Hg (HYP). End-test power (EP; synonymous with critical power) was calculated as the mean power output over the last 30 s of the 3MT, and the work done above EP (WEP; synonymous with W') was calculated as the power-time integral above EP. RESULTS: At the start of the 3MT, capillary blood PCO2 and [H+] were lower in HYP (25.2 ± 3.0 mm Hg, 27.1 ± 2.6 nmol·L-1) than CONT (43.2 ± 2.0 mm Hg, 40.0 ± 1.5 nmol·L-1) (P < 0.001). At the end of the 3MT, blood PCO2 was still lower in HYP (35.7 ± 5.4 mm Hg) than CONT (40.6 ± 5.0 mm Hg) (P < 0.001). WEP was 10% higher in HYP (19.4 ± 7.0 kJ) than CONT (17.6 ± 6.4 kJ) (P = 0.006), whereas EP was 5% lower in HYP (246 ± 69 W) than CONT (260 ± 74 W) (P = 0.007). The ΔWEP (J·kg-1) between CONT and HYP correlated positively with the PCO2 immediately before the 3MT in HYP (r = 0.77, P = 0.006). CONCLUSION: These findings suggest that acid-base changes elicited by prior VH increase WEP but decrease EP during the all-out 3MT.

Analysing patterns of coordination and patterns of control using novel data visualisation techniques in vector coding.

OBJECTIVE: Vector coding is a non-linear data analysis technique that quantifies inter-segmental coordination and coordination variability. The traditional approach of reporting time-series data from vector coding can be problematic when overlaying multiple trials on the same illustration. The objective of this study was to describe and present novel data visualisations for displaying the coordination pattern, segmental dominancy, range of motion on an angle-angle diagram, and coordination variability. This allows for a comparison of data across multiple participants with a focus on single subject analysis. METHODS: Novel data visualisation techniques that involve the use of colour and data bars to map and profile coordination pattern and coordination variability data. The introduction and profiling of inter-data point range of motion quantifies range of motion of the dominant segment on an angle-angle plot and illustrates patterns of movement control. As an example, the dataset used the Istituto Ortopedico Rizzoli foot model to describe rearfoot-forefoot and shank-foot coordination during stance. RESULTS: The use of colour mapping provides the option to inspect an entire dataset and to compare data across multiple participants, groups, and segment couplings. Combining coupling angle mapping with segmental dominancy profiling offers an intuitive and instant summary on coupling angle distribution. The novel inclusion of inter-data point range of motion profiling provides meaning to the interpretation of segmental dominancy data and demonstrates distinct patterns of movement control. CONCLUSIONS: The use of colour mapping and profiling techniques highlighted differences in coordination pattern and coordination variability data across several participants that questions the interpretation and relevance of reporting group data. Colour mapping and profiling techniques are ideal reporting methods to compliment prospective multiple single-subject design studies and to classify commonalities and differences in patterns of coordination and patterns of control between individuals or trials. The data visualisation approaches in the current study may provide further insight on overuse injuries, exercise prescription and rehabilitation interventions.

Thigh-Derived Inertial Sensor Metrics to Assess the Sit-to-Stand and Stand-to-Sit Transitions in the Timed Up and Go (TUG) Task for Quantifying Mobility Impairment in Multiple Sclerosis.

Introduction: Inertial sensors generate objective and sensitive metrics of movement disability that may indicate fall risk in many clinical conditions including multiple sclerosis (MS). The Timed-Up-And-Go (TUG) task is used to assess patient mobility because it incorporates clinically-relevant submovements during standing. Most sensor-based TUG research has focused on the placement of sensors at the spine, hip or ankles; an examination of thigh activity in TUG in multiple sclerosis is wanting. Methods: We used validated sensors (x-IMU by x-io) to derive transparent metrics for the sit-to-stand (SI-ST) transition and the stand-to-sit (ST-SI) transition of TUG, and compared effect sizes for metrics from inertial sensors on the thighs to effect sizes for metrics from a sensor placed at the L3 level of the lumbar spine. Twenty-three healthy volunteers were compared to 17 ambulatory persons with MS (PwMS, HAI ≤ 2). Results: During the SI-ST transition, the metric with the largest effect size comparing healthy volunteers to PwMS was the Area Under the Curve of the thigh angular velocity in the pitch direction-representing both thigh and knee extension; the peak of the spine pitch angular velocity during SI-ST also had a large effect size, as did some temporal measures of duration of SI-ST, although less so. During the ST-SI transition the metric with the largest effect size in PwMS was the peak of the spine angular velocity curve in the roll direction. A regression was performed. Discussion: We propose for PwMS that the diminished peak angular velocity during SI-ST directly represents extensor weakness, while the increased roll during ST-SI represents diminished postural control. Conclusions: During the SI-ST transition of TUG, angular velocities can discriminate between healthy volunteers and ambulatory PwMS better than temporal features. Sensor placement on the thighs provides additional discrimination compared to sensor placement at the lumbar spine.

Association between Match Activity Variables, Measures of Fatigue and Neuromuscular Performance Capacity Following Elite Competitive Soccer Matches.

The aim of the study was to assess the relationships between match activity variables, subsequent fatigue and neuromuscular performance capacity in elite soccer players. Subjects (n = 10) were professional soccer players participating in the English Championships. Match activity variables and markers of fatigue status were measured before and following two matches. Creatine kinase (CK) and muscle soreness were measured at baseline, immediately following, as well as 40 and 64 h post-match. Countermovement jump performance and perceived ratings of wellness were measured at baseline, then 40 and 64 h post-match. Relationships were shown between CK and the total number of accelerations and decelerations immediately (r = 0.63; large), 40 h (r = 0.45; moderate) and 64 h post-match (r = 0.35; moderate) (p < 0.05). Relationships between CK and total sprint distance (r = 0.39; moderate) and the number of sprints (r = 0.35; moderate) 40 h post-match (p < 0.05) were observed. Furthermore, relationships were shown between the perceived rating of wellness and number of accelerations 40 (r = 0.52; large) and 64 h (r = 0.40; moderate) post-match, sprint distance 40 h post-match (r = 0.40; moderate) and the total number of sprints 40 h post-match (r = 0.51; large) (p < 0.05). The quantification of match activity variables, particularly the total number of accelerations and decelerations and the number of sprints, provides insights into the fatigue status in elite soccer players 40 and 64 h post-match.

Three-dimensional kinematics of the lumbar spine during gait using marker-based systems: a systematic review.

To review the current scientific literature on the assessment of three-dimensional movement of the lumbar spine with a focus on the utilisation of a 3D cluster. Electronic databases PubMed, OVID, CINAHL, The Cochrance Library, ScienceDirect, ProQuest and Web of Knowledge were searched between 1966 and March 2015. The reference lists of the articles that met the inclusion criteria were also searched. From the 1530 articles identified through an initial search, 16 articles met the inclusion criteria. All information relating to methodology and kinematic modelling of the lumbar segment along with the outcome measures were extracted from the studies identified for synthesis. Guidelines detailing 3D cluster construction were limited in the identified articles and the lack of information presented makes it difficult to assess the external validity of this technique. Scarce information was presented detailing time-series angle data of the lumbar spine during gait. Further developments of the 3D cluster technique are required and it is essential that the authors provide clear instruction, definitions and standards in their manuscript to improve clarity and reproducibility.

Multi-segment kinematic model to assess three-dimensional movement of the spine and back during gait.

BACKGROUND: Relatively little is known about spine during gait compared to movement analysis of the lower extremities. The trunk is often regarded and analysed as a single rigid segment and there is a paucity of information on inter-segmental movement within the spine and its relationship to pelvis and lower limbs. OBJECTIVES: To develop and validate a new multi-segment kinematic model to assess regional three-dimensional movement of the lumbar, lower thoracic and upper thoracic spine during gait. STUDY DESIGN: Observational study. METHODS: The study was conducted in two parts: (1) to provide validation measures on the kinematic model built in commercially available software and (2) to apply the marker configuration to the spine at T3, T8 and L3 during gait analysis on 10 healthy male volunteers. RESULTS: Proposed model revealed excellent concurrent validation measures between an applied input angle to the recorded output angle from the kinematic model. A high reliability was observed during gait analysis, both during a single session and between sessions for all participants. CONCLUSION: The thoracic region of the spine should not be modelled as a single rigid segment and the proposed three-dimensional cluster is reliable and repeatable to assess the inter-segmental movement of the spine. CLINICAL RELEVANCE: Reliable kinematic data can be collected using the three-dimensional cluster technique, thus, allowing researchers to accurately distinguish between movement patterns of healthy individuals to those with a clinical condition, and provide confidence in data acquisition during the monitoring process of an implemented rehabilitation intervention programme.

A new coordination pattern classification to assess gait kinematics when utilising a modified vector coding technique.

A modified vector coding (VC) technique was used to quantify lumbar-pelvic coordination during gait. The outcome measure from the modified VC technique is known as the coupling angle (CA) which can be classified into one of four coordination patterns. This study introduces a new classification for this coordination pattern that expands on a current data analysis technique by introducing the terms in-phase with proximal dominancy, in-phase with distal dominancy, anti-phase with proximal dominancy and anti-phase with distal dominancy. This proposed coordination pattern classification can offer an interpretation of the CA that provides either in-phase or anti-phase coordination information, along with an understanding of the direction of segmental rotations and the segment that is the dominant mover at each point in time. Classifying the CA against the new defined coordination patterns and presenting this information in a traditional time-series format in this study has offered an insight into segmental range of motion. A new illustration is also presented which details the distribution of the CA within each of the coordination patterns and allows for the quantification of segmental dominancy. The proposed illustration technique can have important implications in demonstrating gait coordination data in an easily comprehensible fashion by clinicians and scientists alike.

The effect of an intervention program on functional movement screen test scores in mixed martial arts athletes.

This study assessed the basic fundamental movements of mixed martial arts (MMA) athletes using the functional movement screen (FMS) assessment and determined if an intervention program was successful at improving results. Participants were placed into 1 of the 2 groups: intervention and control groups. The intervention group was required to complete a corrective exercise program 4 times per week, and all participants were asked to continue their usual MMA training routine. A mid-intervention FMS test was included to examine if successful results were noticed sooner than the 8-week period. Results highlighted differences in FMS test scores between the control group and intervention group (p = 0.006). Post hoc testing revealed a significant increase in the FMS score of the intervention group between weeks 0 and 8 (p = 0.00) and weeks 0 and 4 (p = 0.00) and no significant increase between weeks 4 and 8 (p = 1.00). A χ analysis revealed that the intervention group participants were more likely to have an FMS score >14 than participants in the control group at week 4 (χ = 7.29, p < 0.01) and week 8 (χ = 5.2, p ≤ 0.05). Finally, a greater number of participants in the intervention group were free from asymmetry at week 4 and week 8 compared with the initial test period. The results of the study suggested that a 4-week intervention program was sufficient at improving FMS scores. Most if not all, the movements covered on the FMS relate to many aspects of MMA training. The knowledge that the FMS can identify movement dysfunctions and, furthermore, the fact that the issues can be improved through a standardized intervention program could be advantageous to MMA coaches, thus, providing the opportunity to adapt and implement new additions to training programs.

Quantifying lumbar-pelvis coordination during gait using a modified vector coding technique.

The complexity of human gait patterns has become a topic of major interest in motor control and biomechanics. Range of motion is still the preferred method to quantify movement impairment, however, within these traditional linear measures, the inter-segmental coordination and movement variability is normally ignored. A dynamical systems approach using vector coding and circular statistics provides non-linear techniques to quantify coordination and variability. This study provides comprehensive vector coding and circular statistics calculations. Additionally, pelvis-lumbar coordination and coordination variability data obtained from ten healthy young male participants during five walking trials using an optoelectronic system is provided. This novel data can form the baseline information for future studies in this area of research. Finally, a new illustration to present coordination and coordination variability information of gait kinematics, combining the output from the modified vector coding technique with traditional time-series segmental angle data is presented. This technique, when applied to single patients can be beneficial to assess the effect of an intervention on the patient-specific inter-segmental coordination pattern with implications to the clinical setting.

The acute effect of different warm-up protocols on anaerobic performance in elite youth soccer players.

The purpose of the study was to investigate the acute effect of different warm-up protocols on anaerobic performance in elite youth soccer players. Twenty elite youth soccer players (mean age 17.2 +/- 1.2 years) performed 3 different warm-up protocols in a random order on nonconsecutive days. Each warm-up protocol consisted of a 5-minute low-intensity jog followed by 10 minutes of static stretching (SS), dynamic stretching (DS), or dynamic stretching followed by 8 front squats + 20% body mass (DSR). Subjects performed a countermovement jump followed by a 10- and 20-m sprint test immediately and at 3 and 6 minutes after each warm-up protocol. Vertical jump performance following DSR was better at 3 and 6 minutes than after DS, which in turn was better than after SS at 0, 3, and 6 minutes (p < 0.05). Jump performance was better at 3 minutes than immediately after, and this improvement was maintained at 6 minutes after DSR (p < 0.05). A better sprint performance was observed after DSR and DS compared with SS immediately and at 3 and 6 minutes following each warm-up protocol (p < 0.05). The results of the study suggest that a dynamic warm-up with the inclusion of resistance enhances jumping ability more than dynamic exercise alone. In addition, a dynamic warm-up produces a superior sprint and jump performance compared to a warm-up consisting of static stretching.