{5-Chloro-2-[(4-nitro-benzyl-idene)amino]-phen-yl}(phen-yl)methanone.

The mol-ecule of the title Schiff base compound, C(20)H(13)ClN(2)O(3), assumes an E configuration about the C=N bond. The aromatic rings of the nitro-benzene and chloro-benzene groups are twisted by 13.89 (13)° and form dihedral angles of 76.38 (13) and 84.64 (13)°, respectively, with the phenyl ring. In the crystal, mol-ecules are linked into chains parallel to the b axis by C-H⋯π inter-actions.

{5-Chloro-2-[(2-hy-droxy-benzyl-idene)amino]-phen-yl}(phen-yl)methanone.

The title Schiff base compound, C(20)H(14)ClNO(2), adopts an E configuration about the azomethine bond. The phenol and chloro-benzene rings form dihedral angles of 84.71 (9) and 80.70 (8)°, respectively, with the phenyl ring and are twisted by 15.32 (8)° with respect to one another. The mol-ecular conformation is stabilized by an intra-molecular O-H⋯N hydrogen bond, which forms an S(6) ring motif. In the crystal, mol-ecules are linked by C-H⋯O hydrogen bonds, forming columns parallel to the a axis.

Validated HPLC-RI Method for the Determination of Lactulose and its Process Related Impurities in Syrup.

A simple, swift with good sensitivity and reproducibility, HPLC-RI method has been developed for the quantification of lactulose and related compounds (fructose, galactose, epilactose and lactose) in oral suspension formulation. The analysis was carried out by using mobile phase (water and acetonitrile 75:25) at the flow rate of 1.0 ml/min. on isocratic HPLC-RI system. After manipulating mobile phase composition and mobile phase flow rate a good separation of five components was achieved within 15 minutes of run time. This study is beneficial to determine the active ingredient as well as related compounds simultaneously, without using buffer in mobile phase which causes bad resolution and has limitation to analyze on other hyphenated techniques such as LC-MS.