Bacterial cell permeability study by metal oxide and mixed metal oxide nanoparticles: analysis of the factors contributing to the antibacterial activity of nanoparticles.

In this work, we investigate the nanoparticle-cell wall interaction by NiO and mixed metal oxide CuO-NiO nanoparticles. We have synthesized and characterized the nanoparticles using XRD, FESEM, EDS, UV vis. spectroscopy, FTIR, Zeta, and TEM analysis in our previous work. Furthermore, a preliminary antibacterial study showed that both the nanoparticles performed very well as antibacterial agents. In this extended work, we investigate the mechanism of interaction of NiO and CuO-NiO nanoparticles with S. aureus and E. coli cells as there are number of studies for antibacterial mechanism of CuO nanoparticles. The uptake of crystal violet dye in the outer bacterial membrane, the release of ß-galactosidase enzyme, and relative electric conductivity assay were used to investigate changes in the permeability and integrity of the cell membrane. Superoxide ions, which are produced intracellularly as ROS by nanoparticles, severely damage bacterial membranes. Zeta potential measurement, which resulted in surface charge neutralization, proved membrane instability. FTIR analysis was used to identify changes in the proteins, carbohydrates, and fatty acids that make up the chemical composition of cell surfaces. AFM imaging demonstrated extensive alteration of the nanomechanical and surface characteristics. Confocal microscopy examination supported the DNA fragmentation and nanoparticle-cell adhesion. Due to their enhanced antibacterial activity when compared to monometallic oxide nanoparticles, this study demonstrated that mixed metal oxides can be employed in the health and biomedical sectors.

Remediation and immobilization of Cr(VI)-contaminated soil using stabilized nanoscale iron sulfide and ecological impact.

Soil contaminated with hexavalent chromium seriously threatens the environment and human health. The use of FeS, which has a high redox activity and excellent reduction capacity, limits its application in soil remediation due to its premature surface oxidation and massive aggregation. To prevent premature surface oxidation and agglomeration, cetyltrimethylammonium bromide-supported nano-ferrous sulfide (CTAB-nFeS) was chemically synthesized and used for immobilizing Cr(VI) in contaminated soil. In order to evaluate the role of CTAB stabilization of nFeS and interaction mechanisms were investigated by XPS, FTIR, XRD, and FESEM. Batch experiments showed a complete reduction of Cr(VI) within 3 h with only 235% excess of CTAB-nFeS at a soil pH of 8 compared to days as reported in the literature with alternative FeS forms. The reduction kinetic data could be satisfactorily fitted into the second-order rate model. The rate constant linearly depends on the soil-to-water ratio, but its logarithmic form is linear in the given pH range. The oxidation-reduction potential increases with decreasing initial pH, thus positively impacting the reduction process. XPS analysis revealed the reduction process as multi-steps (reduction, adsorption, and co-precipitation). Ecological studies showed improved plant growth and earthworm survival rate in the remediated soil. Medium-term stability experiments suggested a significant decrease in TCLP leachate concentration of Cr after CTAB-nFeS treatment and remained stable for 60 d. Overall results of our study suggested a sustainable, feasible, and effective strategy for in-situ remediation of Cr(VI)-contaminated soil using CTAB-nFeS at natural pH.

Accelerated radical generation from visible light driven peroxymonosulfate activation by Bi2MoO6/doped gCN S-scheme heterojunction towards Amoxicillin mineralization: Elucidation of the degradation mechanism.

A novel S-scheme photocatalyst Bi2MoO6 @doped gCN (BMO@CN) was prepared through a facile microwave (MW) assisted hydrothermal process and further employed to degrade Amoxicillin (AMOX), by peroxymonosulfate (PMS) activation with visible light (Vis) irradiation. The reduction in electronic work functions of the primary components and strong PMS dissociation generate abundant electron/hole (e-/h+) pairs and SO4*-,*OH,O2*-reactive species, inducing remarkable degeneration capacity. Optimized doping of Bi2MoO6 on doped gCN (upto 10 wt%) generates excellent heterojunction interface with facile charge delocalization and e-/h+ separation, as a combined effect of induced polarization, layered hierarchical structure oriented visible light harvesting and formation of S-scheme configuration. The synergistic action of 0.25 g/L BMO(10)@CN and 1.75 g/L PMS dosage can degrade 99.9% of AMOX in less than 30 min of Vis irradiation, with a rate constant (kobs) of 0.176 min-1. The mechanism of charge transfer, heterojunction formation and the AMOX degradation pathway was thoroughly demonstrated. The catalyst/PMS pair showed a remarkable capacity to remediate AMOX-contaminated real-water matrix. The catalyst removed 90.1% of AMOX after five regeneration cycles. Overall, the focus of this study is on the synthesis, illustration and applicability of n-n type S-scheme heterojunction photocatalyst to the photodegradation and mineralization of typical emerging pollutants in the water matrix.

Enhanced photodegradation of reactive dyes in textile effluent with CoFe2O4/g-CN heterostructure-mediated peroxymonosulphate activation.

Graphitic carbon nitride (g-C3N4) was employed as a sacrificial substructure and two-dimensional support to develop magnetic cobalt ferrite-carbon nitride (CoFe2O4/g-CN) composite via a one-step solid combustion method. The catalyst activated peroxymonosulphate (PMS), through the interconversion of Co2 + /3+|surf. and Fe2 + /3+|surf. on its surface for degradation of reactive dyes (RDs). Excellent ferromagnetic nature (44.15 emu g-1) of the catalyst led to its efficient magnetic separation. With an optimum catalyst and PMS dose of 0.4 g L-1 and 1.5 g L-1, 99% RD removal was achieved for textile effluent (pH 9.5-10), under UV irradiation (48 W). In-depth radical scavenging experiments and EPR analysis confirmed the dominance of radical-based degradation process. Plausible degradation and mineralization pathways of RDs were proposed through identification of intermediates by LCMS/MS analysis. In brief, this study elucidates an exclusive strategy towards the use of g-C3N4 as fuel for facile synthesis of magnetic CoFe2O4/g-CN as a remarkable photocatalyst for activation of PMS towards mineralization of various industrially relevant RDs.

An insight into the mechanism of antibacterial activity by magnesium oxide nanoparticles.

The exact mechanism behind the antibacterial efficacy of nanoparticles has remained unexplored to date. This study aims to shed light the mechanism adopted using magnesium oxide nanoparticles prepared in ethyl alcohol against gram-negative and gram-positive bacterial cells, and the generation of reactive oxygen species (ROS) is proposed to be the dominant mechanism. This paradigm is supported by the quantification of the hydroxyl radical and superoxide anions produced in the nanoparticle treated and untreated bacterial solutions, and by the reduction of the antibacterial efficiency after the addition of a radical scavenger. The production of free Mg2+ ions from the nanoparticle is supposed to be the causative agent behind this uncontrolled ROS generation, resulting in excessive oxidative stress, which the antioxidants of the bacterial cells are unable to nullify, leading to cell damage. The amount of proteins, carbohydrates and lipids leaked due to the distortion of the cellular membrane is also quantified, and it is observed that their leakage trend varies on the structure of the bacterial cell. FESEM images taken at certain time intervals show the gradual internalization of the nanoparticles, and increasing rupture of bacterial cell membranes, leading to cell necrosis.

Multicomponent transport model-based scaling up of long-term fixed bed adsorption of reactive dyes from textile effluent using aminated PAN beads.

Novel functionalized polymeric beads have been prepared by a simple phase inversion technique and its potential as an effective sorbent for reactive dyes is studied. Polyacrylonitrile was used as the base polymer for the beads that were further functionalized using diethylenetriamine. Scanning electron microscopy, FTIR spectroscopy, BET technique, TGA analysis, and zeta potential measurement were used for characterization of the functionalized beads. The adsorption characteristics of the beads were analyzed through adsorption isotherms. A first-principle-based pore diffusion-adsorption model was employed to study adsorption process of the functionalized beads and to determine various mass transfer parameters, i.e., mass transfer coefficient and effective pore diffusivity, in both single and multicomponent cases. For different reactive dyes, the beads have adsorption capacities in the range of 170-230 mg/g. Effects of different operating parameters, i.e., inlet concentration of solute, influent rate, and bed depth were studied to determine the breakthrough performance of the columns prepared with the beads. Industrial dye effluent, containing four reactive dyes at different initial concentrations, was used to study multicomponent adsorption in the columns. The regeneration efficiency of the beads was determined using aqueous cationic surfactant solution. Finally, scaling up of the fixed bed columns was carried out using a first principle-based transport model based on pore diffusion-adsorption processes.

Adsorptive removal of heavy metals from battery industry effluent using MOF incorporated polymeric beads: A combined experimental and modeling approach.

In this study, the metal organic framework (MOF) ZIF-8 was investigated as potential adsorbent for heavy metal ions. The MOF powder was used further to prepare mixed matrix beads (MMBs) using polysulfone as the base material. Both the MOF powder and the MMBs were characterized using Field emission scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, X-ray photoelectron spectroscopy, Brunauer-Emmett-Teller (BET) analyzer and zetasizer. Adsorption capacity of the MMBs were 164-220 mg/g for Pb and 92-161 mg/g for Cd. A fundamental pore diffusion-adsorption model was used to predict the batch kinetics for both single and multicomponent cases and effective pore diffusivities and mass transfer coefficients were determined. Mutual interactions among heavy metals were quantified using interaction parameters. ZIF-8, incorporated in the PSF matrix, plays the predominant role in capturing the metal ions through surface complexation with the NH and metal-OH groups. A first principle-based model involving convection, diffusion and adsorption was used to quantify the breakthrough behavior for the continuous fixed bed column using the MMBs. The column performance was tested with battery industry effluent. The saturated beads were suitably regenerated using 0.1(M) HCl solution. Finally, the model parameters were used for scaling up of the columns.

Activated biochar derived from Opuntia ficus-indica for the efficient adsorption of malachite green dye, Cu+2 and Ni+2 from water.

Applicability of biochar in water treatment is gaining interest due to its sustainability and low production cost. Herein, the biochar (BC) and activated biochar (ABC) synthesized from the cladodes of Opuntia ficus-indica (OFI) cactus were evaluated as a renewable adsorbent for adsorption of organic as well as inorganic pollutants including malachite green (MG) dye, Cu+2 and Ni+2 heavy metals. The modification of biochar with NaOH resulted higher surface basicity regarding more oxygen containing functional groups on the surface. The maximum uptake of 1341 mg g-1, 49 mg g-1 and 44 mg g-1 onto activated biochar for malachite green dye, Cu+2 and Ni+2 was acquired through the best fitted Langmuir isotherm model. Pseudo-second-order and Elovich models were found to provide a suitable fit indicating towards the chemisorption of all three components. Film diffusion and chemisorption are the main steps in adsorption of MG dye and heavy metals on activated biochar. The adsorption mechanisms were also hypothesized for adsorption of MG dye, Cu+2 and Ni+2. The remarkable adsorption capacities with higher reusability characteristics for adsorption of organic pollutants as well as inorganic heavy metals entrusts this activated biochar as a potential cost-effective adsorbent to mitigate water pollution issue.

Ultrasound assisted synthesis of Mg-Mn-Zr impregnated activated carbon for effective fluoride adsorption from water.

High fluoride content in the natural water sources is a serious matter of concern and adsorption is recommended as one of the most convenient, affordable and widely applied defluorination technologies. In this study, a novel composite was synthesized by impregnating magnesium (Mg), manganese (Mn) and zirconium (Zr) on powdered activated carbon (AC) for effective fluoride adsorption and the synthesis was made using sonochemical method. The characterization of the prepared adsorbent AC-Mg-Mn-Zr along with individual metal composites AC-Zr, AC-Mg and AC-Mn were done by SEM, EDX, FTIR, XRD and BET analysis to understand the major functional bonds, and changes in surface chemistry after adsorption. The mechanism of the process was discussed through major reactions involved for individual metals. Due to high point of zero charge (pHPZC = 11.9), the adsorbent was able to remove more than 96% of fluoride consistently with only 1 g/L of optimum adsorbent dosage for a wide pH range (2 to 10). The maximum adsorption capacity obtained was 26.27 mg/g within an equilibrium time of 3 h. More than 96% energy saving was achieved in the sonochemical synthesis route compared to conventional precipitation method of synthesis.

Acoustic cavitation induced synthesis of zirconium impregnated activated carbon for effective fluoride scavenging from water by adsorption.

Environmental concern associated with the side effects of high fluoride content in ground water and surface water has prompted the researchers to look for an efficient, convenient and easy method. Considering the potential of a good adsorbent, present study reports the synthesis of a composite by impregnating zirconium on powdered activated carbon (AC) using ultrasound as the tool for synthesis and applying it for fluoride adsorption from water. The nature of the composite was determined through characterization by scanning electron microscopy (SEM), energy dispersive Xray (EDX), Xray diffraction (XRD), N2 adsorption analysis (BET) and Fourier Transform Infrared Spectroscopy (FTIR) analysis. The pHpzc (point of zero charge) of the adsorbent was found to be 5.03; with the optimum pH obtained at 4 for adsorption of strong electronegative fluoride ions. The initial fluoride concentration was varied from 2.5 up to 20 mg.L-1 and the maximum adsorption capacity of 5 mg.g-1 was obtained. A maximum fluoride removal of 94.4% was obtained for an initial concentration of 2.5 mg.L-1 within an equilibrium time of 180 min. The adsorption isotherm followed the Langmuir isotherm model indicating a monolayer adsorption process and the adsorption kinetics followed pseudo second order model. The effects of various coexisting ions (HCO3-, NO3-, SO42-, Cl-) commonly present in the water were found to have negligible impact on the process performance. Conducting the adsorption-desorption studies for five consecutive cycles for an initial fluoride concentration of 10 mg.L-1, the removal efficiency reduced from 86.2 to 32.6%. The ultrasonic method provided an easy route to synthesize the composite in less time and significantly reduced energy consumption by more than 96% compared to the conventional method.

Simultaneous determination of ascorbic acid, dopamine and uric acid by a novel electrochemical sensor based on N2/Ar RF plasma assisted graphene nanosheets/graphene nanoribbons.

A novel nitrogen/argon (N2/Ar) radio frequency (RF) plasma functionalized graphene nanosheet/graphene nanoribbon (GS/GNR) hybrid material (N2/Ar/GS/GNR) was developed for simultaneous determination of ascorbic acid (AA), dopamine (DA) and uric acid (UA). Various nitrogen mites introduced into GS/GNR hybrid structure was evidenced by a detailed microscopic, spectroscopic and surface area analysis. Owing to the unique structure and properties originating from the enhanced surface area, nitrogen functional groups and defects introduced on both the basal and edges, N2/Ar/GS/GNR/GCE showed high electrocatalytic activity for the electrochemical oxidations of AA, DA, and UA with the respective lowest detection limits of 5.3, 2.5 and 5.7 nM and peak-to-peak separation potential (ΔEP) (vs Ag/AgCl) in DPV of 220, 152 and 372 mV for AA/DA, DA/UA and AA/UA respectively. Moreover, the selectivity, stability, repeatability and excellent performance in real time application of the fabricated N2/Ar/GS/GNR/GCE electrode suggests that it can be considered as a potential electrode material for simultaneous detection of AA, DA, and UA.

Effect of temperature pre-exposure on the locomotion and chemotaxis of C. elegans.

The effect of temperature pre-exposure on locomotion and chemotaxis of the soil-dwelling nematode Caenorhabditis elegans has been extensively studied. The behavior of C. elegans was quantified using a simple harmonic curvature-based model. Animals showed increased levels of activity, compared to control worms, immediately after pre-exposure to 30 °C. This high level of activity in C. elegans translated into frequent turns by making 'complex' shapes, higher velocity of locomotion, and higher chemotaxis index (CI) in presence of a gradient of chemoattractant. The effect of pre-exposure was observed to be persistent for about 20 minutes after which the behavior (including velocity and CI) appeared to be comparable to that of control animals (maintained at 20 °C). Surprisingly, after 30 minutes of recovery, the behavior of C. elegans continued to deteriorate further below that of control worms with a drastic reduction in the curvature of the worms' body. A majority of these worms also showed negative chemotaxis index indicating a loss in their chemotaxis ability.

Effective bacterial inactivation using low temperature radio frequency plasma.

Staphylococcus aureus is one of the most common pathogens responsible for hospital-acquired infections. In this study, S. aureus was exposed to 13.56MHz radiofrequency (RF) plasma generated by two different gases namely nitrogen and nitrogen-oxygen mixture and their sterilization efficacies were compared. Nitrogen plasma had a significant effect on sterilization due to generation of ultraviolet (UV) radiation. However, the addition of 2% oxygen showed enhanced effect on the sterilization of bacteria through nitric oxide (NO) emission and various reactive species. The presence of these reactive species was confirmed by optical emission spectroscopy (OES). Scanning electron microscopy (SEM) analysis was carried out to study the morphological changes of bacteria after plasma treatment. From the SEM results, it was observed that the bacterial cells treated by N(2)-O(2) mixture plasma were severely damaged. As a result, a log(10) reduction factor of 6 was achieved using N(2)-O(2) plasma after 5min treatment with 100W RF power.